Whole genome analysis of linezolid resistance in Streptococcus pneumoniae reveals resistance and compensatory mutations

Table 2 Common mutations found between the S. pneumoniae 1974M1 mutant and the transformants resistant to linezolid.

Name/function of genes	Locus Name^a	1974M1^b,c	T-1974T3^b,c,d	T-R6T2^b,e
23S rDNA	spr_rrnaD23S	G2576T	G2576T	G2576T
23S rDNA	spr_rrnaC23S	G2576T	G2576T	G2576T
23S rDNA	spr_rrnaB23S	G2576T	G2576T	G2576T
23S rDNA	spr_rrnaA23S	G2576T	G2576T	G2576T
50S ribosomal protein L3	spr0188	T409C Y137H	T409C Y137H	T409C Y137H
50S ribosomal protein L16	spr0196	A235G I79L	A235G I79L	A235G I79L
ABC transporter ATP-binding subunit	spr1021	G-29T	G-29T
Conserved hypothetical/rRNA methyltransferase	spr0333	G503T S168I
Sodium/hydrogen exchanger family protein	spr0573	G141A T47I
Conserved hypothetical protein	spr0855	G25A A9T
Fibronectin-binding protein-like protein A	spr0868	C617A S206I
Conserved hypothetical protein	spr1115	C29T T10I
Transcription antitermination factor	spr1820	G38T W13L
ABC transporter ATP-binding/membrane-spanning protein	spr1885	T490G S164R
ABC transporter ATP-binding/membrane-spanning protein	spr1887	G-32T

^a The S. pneumoniae loci number (spr#) are according to the annotation of S. pneumoniae R6.
^b When the mutations are within coding regions, the change in amino acids is also indicated in italics.
^c In noncoding sequences, the number preceded by '-' indicates the position upstream of the ATG.
^d DNA of mutant 1974M1 transformed into 1974 (T3 represents third level transformation).
^e DNA of mutant 1974M1 transformed into R6 (T2 represents second level transformation).

ISSN: 1471-2164