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Table 1 Sequences of primers and probes

From: Segmental duplication as one of the driving forces underlying the diversity of the human immunoglobulin heavy chain variable gene region

Tag

Primer (5' to 3')

 
 

Location

 

Probe (5' to 3')

Name

from

to

Forward

Reverse

Nested

 

VHS429

428971

429130

ATATATACAAGATTAGTCCCACAATA

ATACCAGCTCTACTATATATGAAAGTC

TATGCAAGCATTAGATACCCATA

AAACCATGCTGTCGCAGGGTC

VHS437

437246

437398

CACAAAATGCAGATTCACACTC

AGCCTGTTCTAGTCATGGAATCTC

GAAAAGTCCTCAACTGTGGTCA

CCAGGAACGCCTGAGATTCCT

VHS444

444731

444867

CACCCCTTCACAACTGGGGAT

TGGTAGCGCTGTGAGGTGATA

TGGGGCAGGACACAAATCCAA

TACCTGACCACCATGCGTATT

VHS449

449260

449427

GAACAGACAACCTGTGAATGGT

ATTTCTGTTGAACACTTGTATGTCT

AAATGTGCAGTCTATTCATCAAAT

TATCTCGTTTTTGCTCACTCT

VHS454

454027

454155

TATTACCAGGAGACCAAAATCTC

AAAGGAAGGTTGCTGGTAAGG

ATACAGACAAACAGGCGAAGG

GATTGCCTTTCGCCACATAGA

VHS458

458073

458214

CGAAATATAATTCTTGCTCTTCTG

CAGGAGAATTATGAACATTGAGAG

AGCACATCCTAGCATCCCTGT

CATCGGCGTGTTTACATCTAC

VHS462

462778

462952

CGTTAGCTCTGTGAAAGCAGC

TGATTTGTTCCCTTAGTTTCTGG

CAGGTTTACTGTTAAGGAGGTCA

TGGCTACTCAGCGTGCAAATG

VHS468

468656

468843

TTAGCATCTCCTATTTGAGTAGATTT

TCTAGATATTCAGGAGGCTAATACA

TGTTCCACAGCTTAAAGTACAGTT

TGAGTCCGGAGCTCAAAATTA

VHS475

475730

475892

TGTAGTTTCCTTTATTGTGGTGC

AATTCTTCCCAACAGAAAAGTG

TTACTGGTAATACTATCATGGTAGTC

AGGTAGTCTGCCATACACCTT

VHS479

479662

479810

GAAGCAAATTGATTAGTGTGCAG

CAGCTGAATCGCTTTTGGTC

TCTGCTTTGCCTGCTATTCAT

TGAGAAACACACGGGTCTTAT

VHS485

485677

485819

GCTGTCTCCTAAGTAAGTCACAGG

AGGGATTCAGTGCAAATTGAG

ACACACCAACATCACCAACAT

TCTATAGTTCGTTCGAGGAAGT

VHS493

493051

493239

GAGTGAGCCTTAAGTAATCAACAG

CCCATCCCCTCCTTCTTTCTC

TGACAACTTATTCTAGGTAGCAAGA

CTGGGTCTTGTCCATCGATAC

VHS499

499270

499449

CTGAAGCTGCTTAATCACCGT

TGTGTATTGATTTGACAAAACACTA

TCTGCTCTGATGGAATCAGGG

ATCGGTTGACTCTATGTTAGG

VHS504

504031

504171

TGTGGGTTGTTCTCACCATAATA

ACAACTGGATGCACCTCCATA

CCACCAGACACCCTCCAATAC

CACTCCGACACATTATAAGAGA

VHS510

509963

510127

GTCGTGTATCTCACTTTCCACTA

TTTAGTGGGATTTTAGAGAGTACAA

GATGGAGTCCTGATCCCTGCC

TTTCTGTGACCGTGTGTCACT

VHS514

514084

514235

GAGTGTGGACTGATCCATTGC

ATACCATGTGCTGCTGACACC

ATAGAATCCTTGCTTGGGGTCT

CTAAACTGGCCGTAGGAAAGC

VHS518

518664

518809

CAAACCTGTCAGGGCACTTAG

TGACAGTAAACCAGCCTCTCAT

TCCAGGAAGACTCAAGACCAC

CCCCAAGTGGTCGTGAGTCCC

  1. *Oligos are named using the corresponding tag names followed by a letter of F (forward primer), R (reverse primer), N (nested primer), or B (probe).
  2. **From the 5' end of the IGHV region.
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BMC Genomics

ISSN: 1471-2164

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