Assembly* | Reads Used | Total Length | Scaffold N50# |
|---|
male reads | 24.9 Gb (124.5-fold) | 234 Mb | 9.9 kb |
female reads | 5.7 Gb (28.5-fold) | 170 Mb | 12.6 kb |
reference | 14.0 Gb (98.9-fold) | 183 Mb | 31.9 kb → 49.0 kb |
- * There are three versions of initial assemblies: one produced using only male reads, one using only female reads, and both of these assemblies were used to extract candidate B- or neo-Y linked sequences. The reference assembly was produced using all the female reads and part of the male reads, using parameters to maximize the N50 statistics. The reference assembly was subjected to further optimization (designated by '→' in Table 1) by comparative analysis with other Drosophila genomes and gene annotations. # N50: the length L where 50% of all nucleotides in the assembly are contained in contigs/scaffolds of size ≥ L.
