Figure 11

Effect of temperature on expression of 19 genes of mevalonate (MVA), 2-C-methyl-D-erythritol 4-phosphate (MEP) and phenylpropanoid (PP) pathways (pathways associated with picrosides biosynthesis) as analysed by reverse transcriptase-polymerase chain reaction (RT-PCR). Total RNAs were extracted from the leaf tissue of plants exposed to 15°C and 25°C and changes in the abundance of transcripts were analyzed by RT-PCR using gene specific primers. Data shows fold increase in the transcript levels of putative genes at 15°C as quantified by measuring integrated density value (IDV) of amplicon. Data represents average of three separate biological replicates with error bars as standard deviation. Expression of 26 s rRNA was used as control [92]. Additional file 16 details on primers and the PCR conditions used in RT-PCR.