De novo sequencing and characterization of Picrorhiza kurrooa transcriptome at two temperatures showed major transcriptome adjustments

Table 2 Summary of transcriptome data generated on Illumina genome analyzer IIx for leaf tissue of P. kurrooa.

	15°C	25°C	Total/Pooled
Total number of paired-end reads	27,562,496	49,274,224	76,836,720
Number of reads obtained after quality filtering	20,593,412	44,229,272	64,822,684
Number of assembled transcripts	31,338	63,718	74,336
Average length of transcripts (in bp)	403.87	434.39	439.5
Average coverage (%)	64.68	71.26	76.6

The table shows assembly quality when assembled for reads generated at 15°C and 25°C, separately and by pooling the reads obtained at the two temperatures together. The best contigs were found for the pooled data having higher coverage and higher average transcript length

ISSN: 1471-2164