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Table 2 SSR motifs and their frequency in 3′ UTR, 5′ UTR and coding regions

From: A second generation framework for the analysis of microsatellites in expressed sequence tags and the development of EST-SSR markers for a conifer, Cryptomeria japonica

  

Estimated location

 

SSR

motif

3' UTR

5' UTR

coding

undetermined

Total

di

AT

154

70

73

6

303

 

AG

42

53

155

6

256

 

AC

27

18

46

3

94

 

CG

0

0

2

0

2

 

sub-total

223

141

276

15

655

tri

AAG

19

36

285

2

342

 

ATG

19

32

178

1

230

 

AGG

7

49

170

0

226

 

AGC

18

34

129

2

183

 

AAT

55

22

49

2

128

 

ACC

6

7

64

0

77

 

GGC

9

15

38

0

62

 

AAC

11

9

32

0

52

 

ACG

0

1

11

0

12

 

AGT

2

1

4

0

7

 

sub-total

146

206

960

7

1319

tetra

 

61

65

60

8

194

penta

 

180

193

349

19

741

hexa

 

154

225

703

12

1094

compound

 

15

5

35

1

56

  Total

 

779

835

2383

62

4059

  1. SSR location was estimated by inferring coding regions using the prot4EST pipeline [44] and the fasty35 module of the FASTA package [49]. Some of the locations were un-determined because the corresponding SSRs extended over both coding and non-coding regions.