Growth (A), acetate production (B) and glucose consumption (C) profiles during batch fermentation (2x SD, 10% glucose). The strains were grown in the presence of 100 (square), 200 (triangle) and 300 (diamond) mM acetoin (NADPH-Bdhp), 200 mM acetoin (NADH-Bdhp, open triangle) and without acetoin (59A, circle). Growth is expressed in function of the amount of CO2 released determined as described in material and methods. For transcriptome and metabolome analyses, cells were sampled when CO2 reached 6 g/L (indicated by a dotted line). Complete sugar exhaustion was achieved in 36 h for 59A, in 36, 46 and 54 h for NADH-Bdhp in the presence of 100, 200 and 300 mM acetoin, and in 50 h for NADH-Bdhp in the presence of 200 mM acetoin.