Table 2 miRNAs with predicted influence on SERCA2a expression
MicroRNA | ΔG binding (Kcal mol-1) | ΔG 70 nt flanking 3’ of predicted binding site (Kcal mol-1) | ΔG 70 nt flanking 5’ of predicted binding site (Kcal mol-1) | Predicted binding position in 3’-UTR SERCA2a | Perfect SERCA2a complimentarity to “seed” region |
|---|---|---|---|---|---|
miR-122 | −22.0 | −14.9 | −11.2 | 671-692 | partially (1–6) |
miR-199a-3p | −32.1 | −9.1 | −14.1 | 76-97 | no |
−30.7 | −11.1 | −6.9 | 535-556 | no | |
−24.3 | −9.2 | −6.9 | 705-726 | no | |
−22.0 | −7.6 | −11.3 | 259-280 | no | |
−20.5 | −10.1 | −6.6 | 26-47 | no | |
miR-320a | −24.6 | −10.9 | −7.1 | 117-138 | partially (1–5, 7–12) |
−21.7 | −6.9 | −11.1 | 536-557 | partially (1–2, 4–8) | |
−24.8 | −12.3 | −11.4 | 505-526 | partially (1–4, 7–8) | |
−25.5 | −6.9 | −12.9 | 229-250 | no | |
−20.5 | −8.5 | −5.3 | 711-732 | no | |
miR-320b | −24.6 | −10.9 | −7.1 | 117-138 | partially (1–5, 7–12) |
−21.7 | −6.9 | −11.1 | 536-557 | partially (1–2, 4–8) | |
−25.8 | −12.3 | −11.4 | 505-526 | partially (1–4, 7–8) | |
−25.5 | −6.9 | −12.9 | 229-250 | no | |
miR-320c | −23.2 | −6.9 | −12.9 | 230-249 | no |
−23.3 | −12.3 | −11.4 | 507-526 | partially (1–4, 7–8) | |
−19.5 | −7.7 | −5.3 | 713-732 | no | |
miR-320d | −24.4 | −10.1 | −7.1 | 120-138 | partially (1–5, 7–13) |
−19.4 | −6.9 | −11.1 | 539-557 | partially (1–2, 4–8) | |
−22.8 | −6.9 | −12.9 | 231-249 | no | |
−23.1 | −12.3 | −11.4 | 508-526 | partially (1–4, 7–8) | |
−19.2 | −6.4 | −5.3 | 714-732 | no | |
miR-483-5p | −24.7 | −9.8 | −6.8 | 122-143 | no |
−24.1 | −7.6 | −6.3 | 708-729 | no | |
miR-574-3p | −27.8 | −8.6 | −5.3 | 725-746 | yes |
−32.5 | −7.3 | −9.7 | 37-58 | partially (2–5, 7–13) | |
miR-574-5p | −22.7 | −7.3 | −11.0 | 465-487 | yes |
−25.6 | −14.5 | −14.1 | 664-686 | no | |
−30.2 | −12.5 | −0.3 | 740-762 | no | |
−22.9 | −8.0 | −12.3 | 413-435 | no | |
−21.6 | −7.8 | −11.1 | 364-386 | no | |
miR-140-3p | −19.1 | −11.3 | 0.3 | 746-766 | partially (1–4) |
−20.2 | −7.3 | −9.7 | 38-58 | yes | |
−24.2 | −7.7 | −5.3 | 713-733 | yes |