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Table 4 Stability of reference genes in qPCR of tested samples

From: MicroRNAs and cardiac sarcoplasmic reticulum calcium ATPase-2 in human myocardial infarction: expression and bioinformatic analysis

Technology TaqMan Sybr Green
RG tested miR-26b RNU6B RNU48 miR-26b RNU6B
Measurements Cq SD Cq SD Cq SD Cq SD Cq SD
FFPE 26.67 2.95 32.56 2.41 29.36 2.11 28.56 2.99 19.83 1.58
RNAlater 23.63 0.91 30.24 1.27 25.71 0.96 24.46 1.21 14.66 1.22
  1. Legend: RG, reference gene; Cq, tershold/quantification cycle; SD, standard deviation; FFPE, formalin fixed paraffin-embeded tissue; RNAlater, RNAlater stored samples.