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Table 4 Stability of reference genes in qPCR of tested samples

From: MicroRNAs and cardiac sarcoplasmic reticulum calcium ATPase-2 in human myocardial infarction: expression and bioinformatic analysis

Technology

TaqMan

Sybr Green

RG tested

miR-26b

RNU6B

RNU48

miR-26b

RNU6B

Measurements

Cq

SD

Cq

SD

Cq

SD

Cq

SD

Cq

SD

FFPE

26.67

2.95

32.56

2.41

29.36

2.11

28.56

2.99

19.83

1.58

RNAlater

23.63

0.91

30.24

1.27

25.71

0.96

24.46

1.21

14.66

1.22

  1. Legend: RG, reference gene; Cq, tershold/quantification cycle; SD, standard deviation; FFPE, formalin fixed paraffin-embeded tissue; RNAlater, RNAlater stored samples.
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BMC Genomics

ISSN: 1471-2164

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