Reconstruction of triacylglycerol biosynthesis in B. braunii. Deduced enzymes are shown in white boxes, enzymes not represented in the transcriptome of the Showa strain of B. braunii are in grey boxes. AccABC, acetyl-CoA carboxylase, subunits A, B, and C; AccD, acetyl-CoA carboxylase, subunit D; ACAC, multifunctional protein acetyl-CoA carboxylase; FabD, ACP:S-malonyltransferase; ACP, acyl carrier protein; FabH, β-ketoacyl:ACP synthase III; FabB, β-ketoacyl:ACP synthase I; FabF, β-ketoacyl:ACP synthase II; FabG, β-ketoacyl:ACP reductase; FabZ, β-hydroxyacyl:ACP dehydratase; FabI, enoyl-ACP reductase; DesA, stearoyl-ACP δ-9 desaturase; FatA, oleoyl-ACP hydrolase; FadD, long chain fatty acid:CoA ligase; GlpK, glycerol kinase; GPAT, glycerol-3-phosphate O-acyltransferase; LPAAT, lysophosphatidic acid acyltransferase; PAP, phosphatidic acid phosphatase; DGAT, diacylglycerol acyltransferase; PDAT, phospholipid:diacylglycerol acyltransferase; FAD6, Δ-12 fatty acid desaturase; FAD8, Δ-15 fatty acid desaturase; FADS2, Δ-6 fatty acid desaturase; ELOVL5, very long chain fatty acid elongase; KAR, β-ketoacyl-CoA reductase; PHS1, 3-hydroxyacyl-CoA dehydratase; TER, enoyl-CoA reductase, VLC-TE, very long chain fatty acyl-CoA hydrolase. CoA, coenzyme A; LCSFA, long chain saturated fatty acid (C16-18:0); LCUFA, long chain unsaturated fatty acid (C16-18:1 n-9); LC-PUFA, long chain polyunsaturated fatty acid (C16-18:2–4); VLC-PUFA, very long chain polyunsaturated fatty acid (C20-24:2–4), TAG, triacylglycerol.