- Research article
- Open Access
New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis
BMC Genomics volume 14, Article number: 175 (2013)
Mycoplasma hyopneumoniae, Mycoplasma flocculare and Mycoplasma hyorhinis live in swine respiratory tracts. M. flocculare, a commensal bacterium, is genetically closely related to M. hyopneumoniae, the causative agent of enzootic porcine pneumonia. M. hyorhinis is also pathogenic, causing polyserositis and arthritis. In this work, we present the genome sequences of M. flocculare and M. hyopneumoniae strain 7422, and we compare these genomes with the genomes of other M. hyoponeumoniae strain and to the a M. hyorhinis genome. These analyses were performed to identify possible characteristics that may help to explain the different behaviors of these species in swine respiratory tracts.
The overall genome organization of three species was analyzed, revealing that the ORF clusters (OCs) differ considerably and that inversions and rearrangements are common. Although M. flocculare and M. hyopneumoniae display a high degree of similarity with respect to the gene content, only some genomic regions display considerable synteny. Genes encoding proteins that may be involved in host-cell adhesion in M. hyopneumoniae and M. flocculare display differences in genomic structure and organization. Some genes encoding adhesins of the P97 family are absent in M. flocculare and some contain sequence differences or lack of domains that are considered to be important for adhesion to host cells. The phylogenetic relationship of the three species was confirmed by a phylogenomic approach. The set of genes involved in metabolism, especially in the uptake of precursors for nucleic acids synthesis and nucleotide metabolism, display some differences in copy number and the presence/absence in the three species.
The comparative analyses of three mycoplasma species that inhabit the swine respiratory tract facilitated the identification of some characteristics that may be related to their different behaviors. M. hyopneumoniae and M. flocculare display many differences that may help to explain why one species is pathogenic and the other is considered to be commensal. However, it was not possible to identify specific virulence determinant factors that could explain the differences in the pathogenicity of the analyzed species. The M. hyorhinis genome contains differences in some components involved in metabolism and evasion of the host’s immune system that may contribute to its growth aggressiveness. Several horizontal gene transfer events were identified. The phylogenomic analysis places M. hyopneumoniae, M. flocculare and M. hyorhinis in the hyopneumoniae clade.
Mycoplasmas belong to the class Mollicutes, which is a taxon of bacteria that is characterized by the absence of a cell wall, a relatively small genome size and a strong dependence on nutrients supplied by the host environment . More than 120 mycoplasma species have been described, and although they display diverse life styles, most of the species are parasitic, implying the occurrence of different mechanisms by which they interact with host cells. Several mycoplasmas associate with their host cells through adhesins, while others may also invade cells [2–8]. Among mycoplasmas, several species are responsible for human, animal and plant diseases, but some species are considered commensal organisms .
Mycoplasma hyopneumoniae, Mycoplasma flocculare and Mycoplasma hyorhinis are the most important species that have been identified in porcine respiratory systems [9–11]. Based on a 16S rRNA sequence comparison, M. hyopneumoniae and M. flocculare are known to be closely related . M. hyopneumoniae is the etiological agent of porcine mycoplasmal pneumonia, while M. hyorhinis, which causes polyserositis and arthritis, is also frequently found in swine respiratory tracts . M. flocculare is also widespread in swine herds, but no disease has been associated with this species . M. hyopneumoniae can adhere to the cilia of tracheal epithelial cells and causes damage. Although M. flocculare can also adhere to cilia, no resulting damage has been observed, suggesting that M. hyopneumoniae and M. flocculare may possess different adhesins, facilitating the recognition of different receptor sites on the cilia . Additionally, while M. flocculare is restricted to the swine respiratory tract, M. hyopneumoniae and M. hyorhinis can also colonize other sites, such as cardiac or joint tissues [14, 16]. These bacteria can even colonize different hosts; M. hyorhinis has been detected in human carcinoma tissues [17, 18]. The genetic maps of M. flocculare ATCC 27716 and M. hyopneumoniae strain J have been compared, revealing that at least three chromosomal inversions have occurred since the divergence of both species .
In recent years, the genomes of several mycoplasma species have been sequenced. The absence of several metabolic pathways, which was suggested by genetic and biochemical studies , has been confirmed at the genome sequence level. Among the swine-infecting mycoplasmas, the genomes of M. hyopneumoniae (four strains), M. hyorhinis (four strains) and Mycoplasma suis (two strains) have been sequenced, facilitating the comparison of metabolic pathways and evidencing specific mechanisms that can be utilized to survive in different host environments [20–28].
Because the genome sequences of M. flocculare and M. hyopneumoniae strain 7422 have now been completed, the current study presents a comprehensive comparison of the M. flocculare, M. hyopneumoniae and M. hyorhinis genomes. These three mycoplasma species can inhabit swine respiratory tracts. We have assessed the overall genome organizations including analyses of the open reading frame (ORF) clusters (OCs), inversions and rearrangements, and coding capacities, including analyses of encoded metabolic pathways and surface protein repertoires. Potential mechanisms of interaction with host cells are evidenced, and their implications on pathogenicity are discussed. Additionally, a phylogenomic approach using 32 mycoplasma genomes (including two that are reported here for the first time) was implemented to reconstruct the evolutionary history of the swine mycoplasma genomes, individual genes and/or portions of their genomes, including horizontal gene transfer analysis.
Results and discussion
General genome features of M. flocculare and M. hyopneumoniae7422
The M. flocculare and M. hyopneumoniae 7422 genomes are composed of single, circular chromosomes of 763,948 and 899,887 bp with 28.9% and 28.4% GC contents, respectively. The M. flocculare genome, which by assembly remained with 13 gaps, contains 585 coding sequences (CDSs), of which 356 have known functions and 229 are annotated as hypothetical. The M. hyopneumoniae 7422 genome, completely closed in one contig by assembly, comprises 692 CDSs, of which 414 correspond to proteins with known functions and 278 are annotated as hypothetical. The protein-coding regions occupy approximately 87% of each M. flocculare and M. hyopneumoniae 7422 chromosome, and the average ORF length is 1,145 bp. Each genome contains one gene encoding the ribosomal RNAs (rRNAs) 16S and 23S, one gene encoding rRNA 5S and 30 genes encoding the transfer RNAs (tRNAs) representing all 20 amino acids. The general genome features of the five strains, M. hyopneumoniae (7422, 7448, J, 232 and 168), M. flocculare, and M. hyorhinis HUB-1, were compared in this study and are listed in Table 1.
Among the M. flocculare and M. hyopneumoniae 7422 CDSs that encode proteins with known functions, 380 and 403 CDSs, respectively, were classified into COG families comprising 18 functional categories (Table 2). A functional classification based on the KEGG  analysis assigned 351 and 371 CDSs from M. flocculare and M. hyopneumoniae 7422, respectively, into 15 different categories (Table 2). The performance differences produced by COG with respect to KEGG may be attributable to the presence of paralogs. As expected, the general genomic features and similarities in all of the COG and KEGG categories were strikingly similar between M. flocculare, M. hyopneumoniae, and M. hyorhinis, which commonly exhibited small genome sizes, high AT contents, and no two signal transduction proteins (Table 2).
To identify the genes that constitute the core and pan-genome of M. flocculare, M. hyopneumoniae, and M. hyorhinis, we took advantage of the bidirectional best hit (BBH) approach and plotted the data in a Venn diagram (Figure 1). We identified a considerable number of unique (i.e., organism-specific) genes in M. hyorhinis that may underline the phenotypic differences between this species and M. flocculare and M. hyopneumoniae. Including the repertoire of surface proteins (discussed later) and the inositol metabolism pathway, we identified 76 genes that are unique to M. flocculare, 69 to M. hyopneumoniae and 234 to M. hyorhinis.
When compared to other sequenced strains of M. hyopneumoniae, the genome of strain 7422 displays a highly similar gene composition and organization, with the exception of the localization of the integrative conjugative element (ICEH), which is positioned from 139,715 to 162,049 bp in the 7422 genome and from 518,376 to 540,705 bp in the 7448 genome. The similarity between M. hyopneumoniae gene repertories was 88% approximately. The small, but significative difference in the M. hyopneumoniae 7422 genome is the presence of an exclusive region of genes encoding transposases, hypothetical proteins and an ortholog of subtilisin-like serine protease (positioned from 497,277 to 510,210 bp). In comparison to 7422 genome, just one exclusive region was found in the 7448 genome, which is composed of genes encoding hypothetical proteins (positioned from 746,315 to 757,309 bp).
Comparison of OC organization in M. flocculare, M. hyopneumoniae and M. hyorhinisgenomes
The gene-by-gene genome organization of M. flocculare, M. hyopneumoniae 7448 and M. hyorhinis HUB-1 was analyzed, and the gene localization patterns were compared to detect ORFs with order conservation. The ORF cluster composition, organization and localization in the genomes were analyzed to determine the conservation level among the OC organization. Two groups of ORF clusters were created for each species, the OC group (Additional files 1 and 2) and the monocistronic gene (mC) group (Additional file 3). The general features of the OCs organization in the M. flocculare, M. hyopneumoniae and M. hyorhinis genomes are shown in Table 3 and Additional file 4.
A comparison of the OCs arrangements revealed a similar number of OCs among the M. flocculare, M. hyopneumoniae and M. hyorhinis genomes (Table 3). This result suggests that gene organization in M. flocculare and M. hyorhinis also occur preferably in clusters as found in M. hyopneumoniae. Moreover, as previously described for M. hyopneumoniae, the overall ORF distribution within the OCs in M. flocculare and M. hyorhinis is highly variable with respect to the number of ORFs and the functional categories of the encoded products (Additional files 1 and 2).
An analysis of the mC group revealed a different ORF number in the M. flocculare genome when compared to the organization in the M. hyopneumoniae and M. hyorhinis genomes. There were 51, 34, and 30 mCs in the M. flocculare, M. hyopneumoniae and M. hyorhinis genomes, respectively (Additional file 3). Among all of the mCs, seventeen mCs were shared only by the M. flocculare and M. hyopneumoniae genomes. However, only the CDSs encoding an O-sialoglycoprotein endopeptidase (gcp) and an excinuclease ABC subunit C (uvrC) were found to display monocistronic organization in the three genomes (Additional file 5).
A detailed analysis of the organization of each OC demonstrated a high level of conservation between the M. flocculare and M. hyopneumoniae genomes (Additional file 4). Approximately 78% and 46% of the OCs from M. flocculare display total or partial conserved gene distribution when compared to the OCs of M. hyopneumoniae and M. hyorhinis, respectively (Table 4; Additional file 4). Moreover, a comparative analysis of OC cluster organization among the three mycoplasma species revealed the presence of 12 OCs with complete similarity with respect to the ORF repertoires (synteny was not always detected). The majority of these OCs (seven OCs) were composed of two ORFs, with increasing numbers of up to five ORFs. These data are consistent with previous results that suggested that the majority of gene clusters in diverse organisms are formed by a string of two to four genes [30, 31].
Another group of noteworthy OCs was the group classified as partially conserved among the three mycoplasma species. The number of partially conserved gene-order clusters in different genome pairs is shown in Table 4. The M. flocculare and M. hyopneumoniae genomes shared 44 OCs in which the gene string was partially conserved, and 33 OCs of the 44 OCs were classified as partially conserved in all three analyzed species. It is well known that only a few operons are conserved in most bacterial genomes; the classical example of conserved organization involves the ribosomal protein operons . However, a detailed analysis of the 33 partially conserved OCs revealed gene context conservation in the ribosomal operons (see OC744828 in Additional file 4) and in other clusters, such as clusters containing the chromosomal replication initiation protein (DnaA) (see OC744801 in Additional file 4) and the OC containing the cell division protein MraZ (see OC744867 in Additional file 4). In general, the similarity of the gene order (total or partial) among the prokaryotic genomes is maintained via the horizontal transfer of a chromosomal region. Our results suggest that individual genome pairs, such as M. flocculare and M. hyopneumoniae or M. flocculare and M. hyorhinis, share several OCs, which can partially be attributed to horizontal gene transfer.
A detailed, genomic-scale analysis of the OC organization in M. flocculare and M. hyopneumoniae demonstrated that species-specific differences are not present in genes with known function and/or related with pathogenicity (Additional file 6A-D). Apparently the 24 OCs exclusive to M. hyopneumoniae (not found in the M. flocculare genome) encode hypothetical proteins, transport-related proteins, myo-inositol utilization proteins, the integrative conjugative element (ICEH) and an additional copy of the P97 protein (Additional file 6A). In the M. hyorhinis genome, 35 OCs were unique to this species (Table 3; Additional file 6D), and the majority of the ORFs encode hypothetical proteins or products related to variable surface lipoproteins (vlp genes), which have been described as being involved in a complex system involving bacterial-host interactions .
Rearrangements in the M. flocculare, M. hyopneumoniae and M. hyorhinisgenomes
A detailed comparative analysis of genome organization is needed to understand the evolutionary dynamics of prokaryotic genomes. Therefore, a comparative genomic analysis was performed using the M. flocculare contigs (MFL contigs) and M. hyopneumoniae genome, considering the ORF string organization and OC distribution (Additional file 7A-B). Comparisons were also performed between MFL contigs and the M. hyorhinis genome (Additional file 7A-B); however, in this case, both the global alignment and gene-by-gene alignment were not applicable, possibly due to the large number of transpositions and inversions that have occurred in these genomes.
In the comparison between M. flocculare and M. hyopneumoniae, 22 regions (with lengths ranging from 2 to 75 kb) were identified as being involved in inversions or rearrangements (Additional file 7B). Among these regions, only eight showed major rearrangements, although the OC organization was maintained. Notably, OCs containing several of the genes encoding pathogenicity-related proteins, such as lipoproteins and adhesins, were located within these regions. For instance, major rearrangements were observed in gene clusters encoding P97, P102 (MFL contig 13), P60, P69, P37 (MFL contig 34), P216, P76 (MFL contig 20) and the 46 K surface antigen precursor (MFL contig 4). Genes encoding transposases were found adjacent to some of the inverted segments, such as in MFL contig 23 (containing the P146 and MgPa proteins) and MFL contig 15 (containing the P97-like and P102-like adhesins), suggesting a possible role of these transposases in the rearrangements. Our findings in the comparison between the M. flocculare and M. hyopneumoniae genomes are similar to the situation found in the genomes of the two closely related species Mycoplasma pneumoniae and Mycoplasma genitalium, whose genomes can be divided into segments with highly conserved gene organization, although the segments are arranged differently .
The lack of gene-order conservation beyond the operon level even between relatively closely related species has been previously described . Apparently, in closely related mycoplasmas, such as M. flocculare and M. hyopneumoniae, large-scale gene-order conservation is observed, although genome collinearity is disrupted at some points. Chromosomal rearrangements are generally caused by homologous recombination between repeated sequences within the genome [19, 35]. Although the number of genes involved in DNA repair and recombination in mycoplasmas is relatively small , the gene encoding RecA was found in all sequenced mollicute genomes. Recently, the importance of RecA in the antigenic and phase variation of the MgpB and MgpC adhesins in M. genitalium has been demonstrated .
Repertoire of surface proteins encoded by M. flocculare, M. hyopneumoniae and M. hyorhinis
A comparative in silico survey of the repertoire of encoded surface proteins was performed between the genome of the non-pathogenic M. flocculare and the genomes of two pathogenic mycoplasma species that are found in this tissue in the swine respiratory tract, M. hyopneumoniae (represented by the 7448 strain) and M. hyorhinis (represented by the HUB-1 strain). The results of this survey are summarized in Additional files 8 and 9, and the complete generated datasets are presented in Additional files 10, 11 and 12. Of the total of 585 M. flocculare CDSs, 277 (47.5%) were predicted to encode surface proteins; this number was similar to that of M. hyopneumoniae 7448 (292 out of 716; 44.4%) and higher than that of M. hyorhinis HUB-1 (247 out of 654, 37.7%)
(Additional file 10A-C). The proportion of CDSs encoding surface proteins in these species is considerably large considering their small genome sizes. From these surface protein sets, 28 (10.1%), 42 (14.4%), and 44 (17.8%) CDSs are unique to M. flocculare, M. hyopneumoniae and M. hyorhinis, respectively, with respect to the other two species (Additional file 8; Additional file 11A-C).
The repertoire of M. flocculare surface proteins that is not shared with the other two species consists exclusively of hypothetical proteins; although those of M. hyopneumoniae and M. hyorhinis are predominantly composed of hypothetical proteins (73.8% and 59%, respectively), they also include some proteins with assigned functions. Among these proteins with predicted functions, M. hyopneumoniae includes proteins involved in myo-inositol catabolism, a permease and a protein encoded by the integrative conjugative element (ICEH), and M. hyorhinis includes some variable antigens, secretory system components, transporters and lipoproteins.
Based on an E-value cutoff threshold of 1e-6 to define orthology, nearly 90% of the repertoire of M. flocculare surface proteins is shared with M. hyopneumoniae and/or M. hyorhinis (Additional file 8; Additional file 12). Searches using more stringent conditions resulted in not more than a 20% reduction in the numbers of identified orthologs (data not shown). These results are indicative of physiological similarities that would be consistent with the adaptation to the same host environment. Of the shared proteins, approximately 40% have unknown functions (hypothetical proteins), while the other 60% consist of proteins with assigned functions in at least one of the compared species. Notably, many of these shared proteins (46 proteins, marked in bold in Additional file 12) correspond to putative pathogenicity-related genes in M. hyopneumoniae and/or M. hyorhinis; these proteins include several lipoproteins and adhesins that are thought to play a role in virulence despite of the non-pathogenic nature of M. flocculare and the pathogenicity differences between M. hyopneumoniae and M. hyorhinis. For instance, M. flocculare contains orthologs for the P97 copy 2 and for the P97-like adhesins of M. hyopneumoniae, although it lacks an ortholog for P97 copy 1. The genomic organization of the P97 copy 2 and P97-like ortholog CDSs are similar in both species with respect to gene clustering (Figure 2); this result suggests that P97 copy 2 and P97-like are ancestral P97 paralogs and that they were present in a common ancestor to M. flocculare and M. hyopneumoniae. A second duplication event, which originated the M. hyopneumoniae P97 copy 1, would have occurred after the divergence of M. hyopneumoniae from M. flocculare.
Additional file 13 lists some adhesins that have been associated with pathogenicity and have been experimentally analyzed [38–43] in M. hyopneumoniae 232. The M. hyopneumoniae 7448 and M. flocculare genomes contain orthologs for all the adhesins with the aforementioned exception of one copy of the P97 and P102 proteins that are absent in M. flocculare (also shown in Figure 2). The gene organization and location was analyzed and, as described in Additional file 13, the regions containing these orthologs are involved in inversions or rearrangements (Additional file 7B) in both mycoplasma species. Specifically, three important adhesins (P216, P159 and P60) display highly conserved gene organization between M. hyopneumoniae 232, 7422, 7448 and 168 strains, but they display inversions and rearrangements in M. flocculare. The participation of M. hyopneumoniae adhesins in host-cell adhesion is a complex process involving specific cleavage events . The set of M. flocculare genes that may be involved in adhesion may not be complete, which would explain the differences in host-cell adhesion with respect to M. hyopneumoniae. These results may explain the presence of orthologs in M. flocculare despite its lack of pathogenic capacity.
The presence of surface virulence determinants even in the non-pathogenic M. flocculare and in a non-pathogenic strain of M. hyopneumoniae (J strain) [21, 45] suggests that their roles in pathogenicity may depend on their expression levels and/or post-translational processing, which may vary . Differences in virulence between species and strains can also be associated with the presence of variants of these proteins with or without some functional domains that are associated with features such as adhesion capacity or antigenicity, which has previously been described for several M. hyopneumoniae and M. hyorhinis virulence factors [40, 41, 47, 48]. For instance, the M. flocculare P97 copy 2 and P97-like orthologs present relatively high overall identities to their M. hyopneumoniae counterparts (53% and 57%, respectively), but in the case of P97 copy 2, the M. flocculare ortholog lacks a domain (R1) regarded to be important for virulence in M. hyopneumoniae; instead, it contains a second R2 domain (Additional file 14). These R1 and R2 repeats are absent from the M. flocculare P97-like protein and its orthologs from M. hyopneumoniae (P97-like adhesin) and M. hyorhinis (P95).
Cell-surface features with implications for virulence may also reside in the 10 to 18% of the repertoires of surface proteins that are not shared between M. flocculare, M. hyopneumoniae and M. hyorhinis. However, because all (in the case of M. flocculare) or most (in the cases of M. hyopneumoniae and M. hyorhinis) of these unshared CDS products are hypothetical or conserved hypothetical proteins, their potential contributions to pathogenicity remain elusive. However, considering the nature of the unshared CDS products annotated in M. hyopneumoniae and M. hyorhinis, these ‘exclusive’ and unknown proteins are likely to include players of processes that are important for pathogen-host interactions, such as proteins involved in secretion, the uptake of certain molecules, conjugation and immune evasion/modulation. The variation in M. hyorhinis surface lipoproteins (Vlp) is considered important to protect the organism from the humoral response and may be a primary adaptive strategy for immune evasion during infection and disease [49, 50]. Therefore, at least some of these proteins are expected to compose a portion of the repertoire of determinants of virulence or avirulence for each species or strain.
An additional comparison of the repertoires of surface proteins from M. flocculare, M. hyopneumoniae and M. hyorhinis was performed based on the COG classification of the predicted surface protein sets for each species (see Additional file 10). The produced COG functional profiles of surface proteins for the three species are summarized in Additional file 9. According to the COG, the functional surface protein profile for M. flocculare is similar to those for M. hyopneumoniae and M. hyorhinis; similar numbers of proteins were assigned to each category for the three species. This similarity was observed even for the U and M categories, which include secretion system components (whose repertoires are virtually equivalent for the three species; data not shown), and for the no-COG category, which included 45-53% of the proteins, most of which (82.2% for M. flocculare, 86.6% for M. hyopneumoniae, and 64.8% for M. hyorhinis) were represented by hypothetical proteins or in the additional category of antigen, adhesin or lipoprotein, in which proteins were included based on their prior immunological or functional characterization according to published studies. Overall, the surface protein set for M. flocculare, taken from the correspondent COG profile, was shown to be very similar to those of M. hyopneumoniae and M. hyorhinis. This result suggests that the species have equivalent genetic backgrounds for metabolic and growth processes. Such functional similarities may be the result of common selective pressures associated with the colonization of the same environment (i.e., the swine respiratory tract).
The L (replication, recombination and repair) and V (defense mechanisms) categories displayed differences; M. flocculare (and M. hyorhinis) contained approximately half of the number of proteins as M. hyopneumoniae. Protein sets assigned to the L category are heterogeneous, and the relative excess of proteins in M. hyopneumoniae corresponds to transposases that cannot be found in either M. flocculare or M. hyorhinis. Conversely, category V is enriched with ATP-binding cassette (ABC) transporter system proteins related to defense mechanisms, such as the M. hyorhinis ABC-type multidrug-like transport system ATP-binding proteins and their orthologs in M. flocculare and M. hyopneumoniae. The remaining transporters, including ABC and non-ABC transporter system components (such as those from the phosphotransferase system; PTS) appear in other COG categories, such as E, G, R or P. However, the overall number of transporters unrelated to defense mechanisms (non-V) is roughly equivalent in the three species, with 50, 49, and 47, in the M. flocculare, M. hyopneumoniae and M. hyorhinis surface protein sets, respectively. Although M. hyorhinis have 19 genes encoding transposases, and M. hyopneumoniae 9 genes, notably, the presence of transposases among M. hyopneumoniae predicted surface proteins may be an artifact due to the occurrence of helical structures in these enzymes ; these helices can be misidentified as transmembrane domains. However, the differential presence of at least some transporter system components is indicative of certain M. hyopneumoniae capabilities that are unavailable in both M. flocculare and M. hyorhinis. A larger number of transport proteins is usually related to a species’ capacity to persist in different tissue environments , but this phenomenon does not seem to apply to M. flocculare, M. hyopneumoniae or M. hyorhinis because they share a large portion of the transporter repertoire. This situation is similar to that observed for secretory system components. However, according to the COG (see below), M. hyopneumoniae has approximately two-fold more transporters associated with defense mechanisms than the other two species. In this aspect, M. hyopneumoniae is more similar to Mycoplasma bovis, which infects the respiratory tract and breast and joint tissues of bovines, than to M. flocculare or M. hyorhinis. The implications of the larger M. hyopneumoniae repertoire of defense mechanisms proteins (COG V) for its survival in the swine respiratory tract have not yet been investigated.
Phylogenomics and the phylogenetics of Mycoplasmatacae
From the entire set of 585 annotated M. flocculare genes, 179 gene sets were retrieved that contained at least one gene representative for each swine mycoplasma analyzed here (BLAST cut off E-10). Overall, 179 ortholog-like files representing different CDSs were concatenated, leading to an aligned file containing 104,097 amino acid residues. The neighbor-joining method (NJ) and maximum parsimony (MP) tree topologies did not differ significantly, especially when major clades were considered (Additional files 15, 16, 17; 18). There was consensus in several aspects (Figure 3). As expected, all of the M. hyopneumoniae strains formed a monophyletic clade. Additionally, the M. hyopneumoniae monophyletic clade was closely related to M. flocculare, with high bootstrap support. Finally, M. hyorhinis is basal to Mycoplasma conjunctivae, M. flocculare and M. hyopneumoniae in all of the phylogenomic trees.
The Mycoplasmataceae species were subdivided into the following clades: bovis (including Mycoplasma agalactiae, M. bovis and Mycoplasma fermentans), hominis (Mycoplasma hominis and Mycoplasma arthritidis), hyopneumoniae (M. hyorhinis, M. conjunctivae, M. flocculare, and M. hyopneumoniae), hemotrophic mycoplasma (Mycoplasma suis and Mycoplasma haemofelis), genitalium-pneumoniae (Mycoplasma gallisepticum, Mycoplasma genitalium, and Mycoplasma pneumoniae), and mycoides (Mycoplasma putrefaciens, Mycoplasma capricolum, Mycoplasma leachii, and Mycoplasma mycoides). All of these clades displayed high bootstrap values. The synoviae-pulmonis (M. crocodyli, M. synoviae, and M. pulmonis) group did not form a monophyletic cluster, but they are closely related to the bovis clade. M. penetrans HF-2 is near the genitalium-pneumoniae clade.
Our mycoplasma phylogenomic tree (Figure 3) corroborated the results that were obtained using the RNA polymerase beta subunit (rpoB), 16S-23S rRNA intergenic transcribed spacer region (ITS), and 16S rRNA genes [54, 55]. M. flocculare, M. hyopneumoniae 7448, and M. hyorhinis HUB-1 were located in the hyopneumoniae clade.
When comparing the M. flocculare, M. hyorhinis HUB-1 and M. hyopneumoniae 7448 genomes, several paralog clusters were identified through the bidirectional best hit (BBH) approach, wherein a paralog cluster was defined as a gene set in which every gene is a BBH with at least one other element. Fourteen of these paralogs (DNA methylase, ATP synthase, ribulose-phosphate 3-epimerase, oligoendopeptidase F, single-strand binding protein, fructose-bisphosphate aldolase, dihydrolipoamide dehydrogenase, glucose-6-phosphate isomerase, lipoate-protein ligase, acyl-carrier-protein phosphodiesterase, lactate dehydrogenase, membrane nuclease lipoprotein, TrsE-like protein, and P97) were submitted to phylogenetic analyses to understand the evolutionary history of those paralogs.
Phylogenetic analyses of mycoplasma DNA methylases, which are enzymes that catalyze the transfer of a methyl group to DNA , contained ancient gene duplications in the hyopneumoniae group, leading each DNA methylase paralog to form a monophyletic group that included M. flocculare and M. hyopneumoniae (Additional file 19). Methylation, in addition to involvement in restriction systems, plays an important role in controlling gene expression, and it is one of the most significant DNA modifications . The N6-adenine methylation is involved in bacterial gene regulation and virulence [58–60]. CpG motifs in bacterial DNA may play a significant pathogenic role in inflammatory lung disease because the proinflammatory effects can be reduced by DNA methylation . Microarray analyses and RT-PCR have demonstrated that the deletion of a C5-cytosine methyltransferase in Helicobacter pylori strains can affect the expression of several genes related to motility, adhesion and virulence .
The ATP synthase phylogeny indicated that M. flocculare, M. hyorhinis HUB-1, and M. hyopneumoniae 7448 cluster according to the ATP synthase subunit, with the alpha subunit presenting a more complex evolutionary pattern (Additional file 20). This enzyme is required to synthesize adenosine triphosphate (ATP), providing energy to the cell. The paralogs found in mycoplasmas are related to different subunits that are required for enzymatic function .
Ribulose-phosphate 3-epimerase interconverts the stereoisomers ribulose-5-phosphate and xylulose-5-phosphate . Its phylogeny revealed that ancient duplications occurred in the hyopneumoniae group. Other gene duplications responsible for the M. hyorhinis GDL-1 and M. hyorhinis HUB-1 split can be observed at the base of this M. hyorhinis clade (Additional file 21). Similarly, recent gene duplications can be observed in the M. hyorhinis clade in the oligoendopeptidase F (Additional file 22) and single-strand binding protein (Additional file 23) phylogenetic trees, which both contain high bootstrap support.
M. flocculare and M. hyopneumoniae 7448 contain two copies of fructose-bisphosphate aldolase, while M. hyorhinis HUB-1 contains only one copy. These duplications likely occurred prior to the diversification of the hyopneumoniae group (Additional file 24). The same process occurred during dihydrolipoamide dehydrogenase evolution, as shown in Additional file 25. M. hyorhinis HUB-1 contains two copies of the dimeric glycolytic enzyme glucose-6-phosphate isomerase, which catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate . The lack of statistical confidence in some tree branches did not facilitate inferences regarding the evolutionary history of these copies in M. hyorhinis HUB-1 (Additional file 26).
Two lipoate-protein ligases are found in M. hyopneumoniae 7448 and M. hyorhinis HUB-1. They are more closely related to the enzymes from other microorganisms than to each other (Additional file 27). In M. flocculare, only one copy was identified. The same pattern was observed in the phylogenetic tree of acyl-carrier-protein phosphodiesterase (Additional file 28), which belongs to the hydrolase family and acts on phosphoric diester bonds . The topology of the lactate dehydrogenase tree showed that the two M. hyorhinis HUB-1 protein copies are significantly different, which led the sequences to be grouped in distant clades (Additional file 29). A recent duplication of membrane nuclease lipoprotein resulted in a M. hyorhinis cluster containing the two M. hyorhinis HUB-1 copies. A unique copy of this specific protein was found in M. flocculare and M. hyopneumoniae 7448 (Additional file 30).
Two copies of the TrsE -like protein were identified in M. hyopneumoniae 7448, and one copy was identified in the M. flocculare and M. hyorhinis HUB-1 genomes. Each copy is more similar to other sequences in the M. hyopneumoniae strain than to one another (Additional file 31). Finally, P97 is an adhesin thought to play a role in virulence. Several copies have been detected in the hyopneumoniae group. The phylogenetic tree indicated that the copies of M. flocculare and M. hyopneumoniae 7448 are more closely related to the sequences from other species and strains than to each other (Additional file 32).
Although mycoplasmas contain reduced genomes, some paralogs are maintained in their genomes. A phylogenetic analysis was conducted to better understand the evolution of those paralogs. Gene copies are known to be preserved in a genome if the organism demands high levels of particular gene products. In other cases, positive selection can result in the diversification of the gene’s function, a process called neofunctionalization . Additionally, subfunctionalization can lead to the loss of function, resulting in duplicated genes whose functions differ to some degree . Even highly conserved genes may have slightly or very different functions, such as glycolytic enzymes such as fructose-bisphosphate aldolase (FBA), which have been described as complex, multifunctional proteins that perform non-glycolytic functions .
Some mycoplasma paralog proteins, such as lipoate- protein ligase may possess different functions or differ in substrate specificity. Otherwise, essential enzymes, such as ATP synthase, may maintain multiple gene copies because they encode different subunits that are required for enzymatic function, despite the recent finds of losses of this enzyme family in the common ancestor of Mollicutes . According to the standard model of phylogenomics, there is a higher similarity among orthologs than paralogs . The paralog genes initially display identical sequences and functions. However, the action of selective pressures and mutations lead to divergence in regulatory and coding sequences .
Horizontal gene transfer
The species tree that was generated by the phylogenomic analysis was compared to the individual gene trees to investigate the occurrence of horizontal gene transfer (HGT) events. HGTs are an important source of genome innovation and evolution in prokaryotes , and it apparently also impacts Mycoplasmataceae evolution.
In mycoplasmas, we observed several HGT events (Additional file 33); some of the events occurred between M. hyorhinis HUB-1 and M. conjunctivae HRC. The events involving species belonging to the hyopneumoniae group occurred in ribosomal proteins, GTP-binding proteins, heat-shock proteins, DNA primase, signal recognition particle protein, ABC transporter ATP-binding proteins, phosphoesterases, cell division protein, elongation factor, fructose-biphosphate aldolase, DNA polymerase, glutamyl-tRNA synthetase, helicases, and hypothetical proteins. Regions encoding ABC transporters were likely transferred between M. synoviae and M. gallisepticum (Additional file 33), corroborating previously published results .
It is well-known that prokaryotes exchange genes in a sophisticated manner via lateral transfer, and bacterial phylogenies may also be viewed as a complex network of genomic exchange. However, the sequence-based methods implemented in the phylogenomic studies have yielded phylogenetic trees that are similar to rRNA trees, which were demonstrated in the current study. Consequently, lateral transfer events do not prevent the recovery of phylogenetic signals in prokaryotes, although they do add an extra source of noise .
Mycoplasmas contain a reduced genome; therefore, they lack many metabolic pathways, particularly biosynthetic pathways, such as those involved in cell-wall production, de novo purine biosynthesis and the biosynthesis of amino acids . These organisms also lack a functional tricarboxylic acid (TCA) cycle because they are extremely fastidious in their nutritional requirements and dependent on nutrients supplied by their hosts. They produce high levels of enzymes responsible for the degradation of nucleic acids and proteins and transporters to obtain the precursors of these macromolecules. Most mycoplasma species depend on the glycolytic pathway to generate ATP. Some species may produce ATP based on the reaction involving acetyl phosphate and ADP by acetate kinase, coupled with acetyl phosphate formation from acetyl-CoA by phosphate acetyl transferase. Acetyl CoA is formed by the pyruvate dehydrogenase complex [1, 74]. Some mycoplasma species such as M. hominis and M. arthritidis produce ATP through arginine degradation, using the arginine dihydrolase pathway . This pathway is absent in the mycoplasma genomes analyzed in this work. All of the genes that encode enzymes of the glycolytic pathway exist in the three species; however, some differences in gene copy number were observed. Two copies of the genes encoding fructose-bisphosphate aldolase exist in M. hyopneumoniae and M. flocculare, two copies of the gene encoding d-ribulose-5-phosphate 3 epimerase are present in M. hyopneumoniae, and two copies of the gene encoding glucose-6-phosphate isomerase are present in M. hyorhinis. The possible influence of the gene copy number on the physiology of the species is unknown. However, some glycolytic enzymes have been described as virulence factors, exhibiting functions unrelated to glycolysis, such as adhesion to the host cells, may contribute to the pathogenesis of mycoplasmas infections [7, 68, 76–79]. The evolutionary aspects of these paralogs are discussed in the “Phylogenomics and phylogenetics of Mycoplasmatacae” section.
It has been shown that nuclease activities can be detected in Mollicutes and that these activities are primarily associated with the membrane and may be essential for growth and survival . Genes encoding nucleases or putative membrane-associated nucleases were found in the three analyzed Mycoplasma species. Two gene sets encoding membrane nucleases were observed in the three species. One set is represented by two copies of mnuA (MHR_0206 and MHR_0549) in the M. hyorhinis HUB-1 genome and a single copy in M. hyopneumoniae 7448 (MHP7448_0580), M. hyopneumoniae 7422 (MX03145) and M. flocculare (MF00420). Another set represented by two copies was observed in the genomes of the three species. The cell surface-exposed exonuclease (mhp379) from M. hyopneumoniae 232, a representative of the latter set, has been analyzed, and it has been proposed that the exonuclease activity of mhp379 may be important for importing nucleic acid precursors . The presence of an extra copy of a nuclease gene in M. hyorhinis may represent a potential advantage of this species in acquiring nucleic acids precursors. Mycoplasmas cannot de novo synthesize purines and pyrimidines; therefore, they depend on salvage and interconversions to supply the cell with the nucleic acid precursors . The three species contain a similar set of genes involved in purine (MHP7448_0084 - M. hyopneumoniae, MHR_0566 - M. hyorhinis and MF01198 - M. flocculare) and pyrimidine (MHP7448_0578 - M. hyopneumoniae, MHR_0640 - M. hyorhinis and MF00424 - M. flocculare) metabolism (See Additional file 4). However, M. hyorhinis contains genes encoding thymidylate synthetase (TS), allowing the conversion of dUMP to dTMP, and dihydrofolate reductase (DHFR), which catalyzes the reduction of dihydrofolate (DHF) to tetrahydrofolate (THF). The presence of TS and DHFR in M. hyorhinis may also contribute to its ability to overgrow the other Mycoplasma species in the swine respiratory tract .
The comparative analyses of three mycoplasma species that inhabit the swine respiratory tract facilitated the identification of some characteristics that may promote the understanding of their different behaviors. The M. hyopneumoniae strain 7422 genome displays a similar organization as to the other previously described strains, but it contains rearrangements and an altered position of ICEH in the genome. The genomes of M. hyopneumoniae and M. flocculare, two closely related species, contain some blocks of synteny, but they also display many differences that may help to explain why one species is pathogenic and the other is commensal. However, it was not possible to correlate specific virulence determinant factors to the pathogenicity differences of the analyzed species. A large proportion of the repertoire of M. flocculare surface proteins is shared with M. hyopneumoniae and/or M. hyorhinis, which would be expected because the organisms may occupy the same niche. However, certain members of the p97 family are absent in M. flocculare, and some display sequence differences or lack domains that are considered to be important for host-cell adhesion. M. hyorhinis contains some metabolic genes that are absent in the other species, suggesting a possible advantage in the growth of this species. The differences in some components involved in evasion of the host immune system may also contribute to the aggressive growth of M. hyorhinis. The phylogenomic analysis confirmed previous results, placing M. hyopneumoniae, M. flocculare and M. hyorhinis in the hyopneumoniae clade. Several horizontal gene transfer events were identified, and several of them occurred between M. hyorhinis and M. conjunctivae.
Bacterial strains, culture conditions, and DNA isolation
M. hyopneumoniae strain 7422 was isolated from an infected swine in Lindóia do Sul, Santa Catarina, Brazil. M. flocculare (ATCC 27716) was acquired by Embrapa Suínos e Aves (Concórdia, Brazil) from the American Type Culture Collection. Both of the strains were cultivated in 5 mL of Friis medium  at 37°C for 48 h, and genomic DNA was extracted according to a standard protocol .
Genome sequencing, assembly and annotation
For each species, one 454 shotgun library was prepared with approximately 5 μg of gDNA. The library construction, titration, emulsion PCR and sequencing steps were performed explicitly according to the manufacturer’s protocol. Sequencing was performed using Roche 454 GS FLX Titanium platform. M. flocculare was sequenced in one region of a two-region PicoTiterPlate (PTP), and M. hyopneumoniae 7422 was sequenced in two regions of an eight-region PTP. The contigs were assembled using the Newbler software program version 2.6 with the default parameters. The PCR assisted contig extension (PACE) strategy  was used for physical gap closure by the ends regions of gaps. For M. hyopneumoniae 7422 and M. flocculare, the estimated genome coverage for both genomes was 23X. The M hyopneumoniae 7422 genome was completely closed in one contig, and the M. flocculare genome retained 13 gaps.
The annotation and analysis of the sequences of both genomes were performed using the System for Automated Bacterial Integrated Annotation (SABIA) . The comparative analysis was based on the Bidirectional Best Hits (BBH)  approach using the BLASTP (Basic Local Alignment Search Tool)  program to identify corresponding gene pairs recognized as the best hits in other genomes. All of the BLASTP searches were performed using the following parameters: an e-value of 10-5, query coverage of 60%, and positive similarity value of 50%. The comparative databank is available at http://www.genesul.lncc.br/comparative/
To store and analyze the data, a databank was developed using the MySQL and Perl programming languages. This databank integrates tools and information from numerous biological databases, such as The Integrated Resource of Protein Domains and Functional Sites (Interpro) , Protein Subcellular Localization Prediction Tool (Psort) , Kyoto Encyclopedia of Genes and Genomes (KEGG) , Clusters of Orthologous Groups of Proteins (COG) , Transporter Classification Database (TCDB) , BLASTP of KEGG and UniProt/Swiss-Prot , facilitating several analyses, such as cluster with minimal genomes and clusters exclusives genes for each genome analyzed. In addition, the databank allows automatic genomic comparisons by bidirectional best hits (BBH) between five species. This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession PRJNA65295 ID: 65295 for M. flocculare and PRJNA47327 ID: 47327 for M. hyopneumoniae 7422.
In silicoanalysis of ORF clusters (OCs)
The prediction of OCs was performed by the Artemis Release 10.5.2 software program  according to previously established criteria . The manual examination of the possible OCs in the M. flocculare, M. hyorhinis and M. hyopneumoniae genomes was established based on the occurrence of clusters with two or more tandem genes in the same DNA strand. This procedure was performed by a systematic annotation comparison of the protein sequences encoded in all of the ORFs from the analyzed genomes. According to the complexity of the adjacent ORF rearrangements, two groups were created; the OC group was characterized by the presence of two or more ORFs in the same DNA strand until the occurrence of ORFs in the opposite strand, and the monocistronic (mC) group represented single ORFs. Differences in the annotation were evaluated by comparing the protein sequences from these three genomes using the NCBI/BLASTP program. The OC groups predicted for M. flocculare and M. hyorhinis were compared with the OC organization found in M. hyopneumoniae. Moreover, comparative analyses were also performed between M. hyorhinis and M. flocculare to predict OC organization. The M. flocculare analysis was performed with each contig sequence, while the M. hyorhinis analysis was performed with the whole genome sequence.
Analysis of surface-protein-encoding CDSs
For the surface protein predictions, all of the translated CDS entries from the M. flocculare genome and from the M. hyopneumoniae 7448 and M. hyorhinis HUB-1 genomes (NC_007332 and NC_014448 entries, respectively) were analyzed using the default parameters by the following software programs: SVMtm Transmembrane Domain Predictor , TMHMM Server v. 2.0 , SCAMPI , and PSORTb . The first three programs predicted the presence of transmembrane (TM) domains, and the fourth was used for to predict subcellular localization (i.e., cytoplasmic vs. membrane). The first three independent TM predictions were merged, and the CDSs were considered as ‘surface protein encoding’ when they were predicted as such by at least two of the TM-predicting programs. The CDSs that were predicted to encode surface proteins by only one of the TM-predicting programs and/or by PSORTb were additionally analyzed by HMM-TM , and when a previously described ortholog was identified, based on the published literature. Those CDSs that were able to predict surface localization either by HMM-TM or based on the literature were also included in the list of predicted surface proteins encoded by each genome. After orthologs were identified by reciprocal pairwise comparisons between M. flocculare, M. hyopneumoniae 7448 and M. hyorhinis HUB-1 (see below), the CDSs predicted to encode surface proteins just for one species (by the aforementioned criteria) had their corresponding orthologs (when existent) included in the list of surface-protein-encoding CDSs for the other two species. The clusters of orthologous group (COG) functional classification of M. flocculare predicted surface proteins using NCBI COGnitor , and those of M. hyopneumoniae 7448 and M. hyorhinis HUB-1 predicted surface proteins obtained from NCBI.
For the ortholog and paralog identifications, bidirectional local BLAST searches  were performed between the M. flocculare predicted surface protein encoding CDSs and all of the CDSs from the other two species using the BioEdit Sequence Alignment Editor . The ortholog and paralog definitions were based on the best hits from TBLASTX with the following parameters: cut-off e-value thresholds of 10-20, 10-10 or 10-6; manual inspection of query coverage, identity and similarity scores; and, if required, consideration of peculiarities of specific gene families.
M. flocculare (ATCC 27716) was used as query organism. We began with all 582 genes identified in this strain and performed a BLAST search against the gene set of all other 32 organisms (Additional file 34). To select putative orthologs for all of the M. flocculare genes, we performed a BLAST search between this queried gene set and the individual genomes of the organisms previously cited; we retrieved the single best hit from each genome (BLAST cut-off 10). Multi-FASTA files were created containing ortholog gene sets for each query gene in the M. flocculare strain. Only gene sets containing at least one representative in each genome were selected for further phylogenomic analysis (i.e., we only evaluated files containing 32 sequences for each analyzed genome).
Multi-FASTA putative ortholog files containing the best representative of each M. flocculare deduced protein sequence for all of the analyzed swine mycoplasmas were used as the input for multiple alignments using the CLUSTALw algorithm with default parameters.
The SCaFos software program  was used to facilitate the concatenation of the 179 alignment files. Phylogenies of 32 concatenated, deduced amino acid sequences were estimated by the NJ  and MP  methods in the Molecular Evolutionary Genetics Analysis (MEGA) program version 5.05 . For the NJ method, the evolutionary distances were computed using the p-distance and the Poisson-corrected amino acids distance; both were presented in units of amino acid differences per site. The complete and pairwise deletion of gaps or missing data were implemented with the datasets containing 49,751 and 104,097 positions, respectively. The bootstrap test of the phylogeny was performed using 1,000 repetitions. The MP tree was obtained using the close-neighbor-interchange algorithm with search level 1, in which the initial trees were obtained by random addition of sequences (10 replicates). The different gap treatments were tested considering the complete deletion, partial deletion, and all sites included. The bootstrap test was implemented using 500 replicates. The TreeView software program  was used to visualize the resulting phylogenies.
Selected gene clusters of paralogs were subjected to a phylogenetic analysis. BLAST searches were first conducted for each gene using a 10-6 e-value cutoff; all of the sequences were subsequently aligned using COBALT . Distance and parsimony methods in the MEGA 5 software program were applied to identify the evolutionary scenario for each paralog cluster. A bootstrap test was additionally performed with 1,000 replications.
Horizontal gene transfer
Horizontal gene transfer (HGT) was analyzed with Tree and Reticulogram reconstruction (T-Rex)  using the bipartition dissimilarity as the optimization criteria in the HGT detection algorithm . The program infers an optimal (i.e., minimum-cost) scenario of horizontal gene transfers reconciling a given pair of species and gene trees. All of the gene trees were obtained through the distance method implemented in the MEGA 5 software program using p-distance, pairwise deletion of gaps, and bootstrap test of phylogeny with 500 replications. A bootstrap cutoff of 75% was applied to accept the HGT events. In total, 179 genes were subjected to phylogenetic and HGT analyses.
Coding DNA sequence
Clusters of orthologous groups
Horizontal gene transfer
Open read frames
Razin S, Yogev D, Naot Y: Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev. 1998, 62: 1094-1156.
Rottem S: Interaction of mycoplasmas with host cells. Physiol Rev. 2003, 83: 417-432.
Zhang S, Lo SC: Effect of mycoplasmas on apoptosis of 32D cells is species-dependent. Current Microbiol. 2007, 54: 388-395. 10.1007/s00284-006-0491-x.
Vogl G, Plaickner A, Szathmary S, Stipkovits L, Rosengarten R, Szostak MP: Mycoplasma gallisepticum invades chicken erythrocytes during infection. Infect Immun. 2008, 76: 71-77. 10.1128/IAI.00871-07.
Dusanic D, Bercic RL, Cizelj I, Salmic S, Narat M, Bencina D: Mycoplasma synoviae invades non-phagocytic chicken cells in vitro. Vet Microbiol. 2009, 138: 114-119. 10.1016/j.vetmic.2009.02.014.
Groebel K, Hoelzle K, Wittenbrink MM, Ziegler U, Hoelzle LE: Mycoplasma suis invades porcine erythrocytes. Infect Immun. 2009, 77 (suppl 2): 576-584.
Song Z, Li Y, Liu Y, Xin J, Zou X, Sun W: α-enolase, an adhesion-related factor of Mycoplasma bovis. PLoS One. 2012, 7 (suppl 6): e38836-
Hopfe M, Deenen R, Degrandi D, Köhrer K, Henrich B: Host cell responses to persistent mycoplasmas - different stages in infection of HeLa cells with Mycoplasma hominis. PLoS One. 2013, 8 (suppl 1): e54219-
Mare CJ, Switzer WP: Mycoplasma hyopenumoniae, a causative agent of virus pig pneumonia. Vet Med. 1965, 60: 841-846.
Meyling A, Friis NF: Serological identification of a new porcine mycoplasma species, Mycoplasma flocculare. Acta Vet Scand. 1972, 13: 287-289.
Rose DL, Tully JG, Wittler RG: Taxonomy of some swine mycoplasmas: Mycoplasma suipneumoniae Goodwin et al. 1965, A later, objective synonym of Mycoplasma hyopneumoniae mare and Switzer 1965, and the status of Mycoplasma flocculare meyling and friis 1972. Int J Syst Evol Microbiol. 1979, 29: 83-91.
Stemke GW, Laigret F, Grau O, Bové JM: Phylogenetic relationships of three porcine mycoplasmas, mycoplasma hyopneumoniae, mycoplasma flocculare and mycoplasma hyorhinis, and complete 16S rRNA sequence of M. Flocculare. Int J Syst Bacteriol. 1992, 42: 220-225. 10.1099/00207713-42-2-220.
Friis NF, Feenstra AA: Mycoplasma hyorhinis in the etiology of serositis among piglets. Acta Vet Scand. 1994, 35: 93-98.
Kobisch M, Friis NF: Swine mycoplasmoses. Rev Sci Tech Oie. 1996, 15: 1569-1605.
Young TF, Thacker EL, Erickson BZ, Ross RF: A tissue culture system to study respiratory ciliary epithelial adherence of selected swine mycoplasmas. Vet Microbiol. 2000, 71: 269-279. 10.1016/S0378-1135(99)00176-5.
Boye M, Jensen TK, Ahrens P, Hagedorn-Olsen T, Friis NF: In situ hybridisation for identification and differentiation of Mycoplasma hyopneumoniae, Mycoplasma hyosynoviae and Mycoplasma hyorhinis in formalin-fixed porcine tissue sections. Acta Path Micro Im. 2001, 109: 656-664.
Huang S, Li JY, Wu J, Meng L, Shou CC: Mycoplasma infections and different human carcinomas. World Gastroentero. 2001, 7: 266-269.
Yang H, Qu L, Ma H, Chen L, Liu W, Liu C, Meng L, Wu J, Shou C: Mycoplasma hyorhinis infection in gastric carcinoma and its effects on the malignant phenotypes of gastric cancer cells. BMC Gastroenterol. 2010, 10: 132-10.1186/1471-230X-10-132.
Blank WA, Stemke GW: A physical and genetic map of the Mycoplasma flocculare ATCC 27716 chromosome reveals large genomic inversions when compared with that of Mycoplasma hyopneumoniae strain J. Int J Syst Evol Micr. 2001, 51: 1395-1399.
Minion FC, Lefkowitz EL, Madsen ML, Cleary BJ, Swartzell SM, Mahairas GG: The genome sequence of strain 232, the agent of swine mycoplasmosis. J Bacteriol. 2004, 186: 7123-7133. 10.1128/JB.186.21.7123-7133.2004.
Vasconcelos ATR, Ferreira HB, Bizarro CV, Bonatto SL, Carvalho MO, Pinto PM, Almeida DF, Almeida LGP, Almeida R, Alves-Filho L, Assunção EN, Azevedo VAC, Bogo MR, Brigido MM, Brocchi M, Burity HA, Camargo AA, Camargo SS, Carepo MS, Carraro DM, de Mattos Cascardo JC, Castro LA, Cavalcanti G, Chemale G, Collevatti RG, Cunha CW, Dallagiovanna B, Dambrós BP, Dellagostin OA, Falcão C: Swine and poultry pathogens: the complete genome sequences of two strains of mycoplasma hyopneumoniae and a strain of mycoplasma synoviae. J Bacteriol. 2005, 187: 5568-5577. 10.1128/JB.187.16.5568-5577.2005.
Liu W, Fang L, Li S, Li Q, Zhou Z, Feng Z, Luo R, Shao G, Wang L, Chen H, Xiao S: Complete genome sequence of Mycoplasma hyorhinis strain HUB-1. J Bacteriol. 2010, 192 (suppl 21): 5844-5845.
Liu W, Feng Z, Fang L, Zhou Z, Li Q, Li S, Luo R, Wang L, Chen H, Shao G, Xiao S: Complete genome sequence of Mycoplasma hyopneumoniae strain 168. J Bacteriol. 2011, 193 (suppl 4): 1016-1017.
Guimaraes AM, Santos AP, SanMiguel P, Walter T, Timenetsky J, Messick JB: Complete genome sequence of Mycoplasma suis and insights into its biology and adaption to an erythrocyte niche. PLoS One. 2011, 6 (suppl 5): e19574-
Oehlerking J, Kube M, Felder KM, Matter D, Wittenbrink MM, Schwarzenbach S, Kramer MM, Hoelzle K, Hoelzle LE: Complete genome sequence of the hemotrophic Mycoplasma suis strain KI3806. J Bacteriol. 2011, 193 (suppl 9): 2369-2370.
Kornspan JD, Lysnyansky I, Kahan T, Herrmann R, Rottem S, Nir-Paz R: Genome analysis of a Mycoplasma hyorhinis strain derived from a primary human melanoma cell line. J Bacteriol. 2011, 193 (suppl17): 4543-4544.
Calcutt MJ, Foecking MF, Rosales RS, Ellis RJ, Nicholas RA: Genome sequence of Mycoplasma hyorhinis strain GDL-1. J Bacteriol. 2012, 194 (suppl 7): 1848-
Goodison S, Urquidi V, Kumar D, Reyes L, Rosser CJ: Complete genome sequence of Mycoplasma hyorhinis strain SK76. Genome Announc. 2013, 1 (suppl 1): e00101-e00112.
Kanehisa M, Goto S: KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 2000, 28: 27-30. 10.1093/nar/28.1.27.
Siqueira FM, Schrank A, Schrank IS: Mycoplasma hyopneumoniae transcription unit organization: genome survey and prediction. DNA Res. 2011, 18: 413-422. 10.1093/dnares/dsr028.
Rogozin IB, Makarova KS, Murvai J, Czabarka E, Wolf YI, Tatusov RL, Szekely LA, Koonin EV: Connected gene neighborhoods in prokaryotic genomes. Nucleic Acids Res. 2002, 30: 2212-2223. 10.1093/nar/30.10.2212.
Lathe WC, Snel B, Bork P: Gene context conservation of a higher order than operons. Trends Biochem Sci. 2000, 25 (suppl 10): 474-479.
Rosengarten R, Wise KS: Phenotypic switching in mycoplasmas: phase variation of diverse surface lipoproteins. Science. 1990, 247: 315-318. 10.1126/science.1688663.
Himmelreich R, Plagens H, Hilbert H, Reiner B, Herrmann R: Comparative analysis of the genomes of the bacteria Mycoplasma pneumoniae and Mycoplasma genitalium. Nucleic Acids Res. 1997, 25: 701-712. 10.1093/nar/25.4.701.
Mahan MJ, Roth JR: Role of recBC function in formation of chromosomal rearrangements: a Two-step model for recombination. Genetics. 1989, 121: 433-443.
Carvalho FM, Fonseca MM, Medeiros SB, Scortecci KC, Blaha CAG, Agnez-Lima LF: DNA repair in reduced genome: the Mycoplasma model. Gene. 2005, 360 (suppl 2): 111-119.
Burgos R, Wood GE, Young L, Glass JI, Totten PA: RecA mediates MgpB and MgpC phase and antigenic variation in Mycoplasma genitalium, but plays a minor role in DNA repair. Mol Microbiol. 2012, 85 (suppl 4): 669-683.
Burnett TA, Dinkla K, Rohde M, Chhatwal GS, Uphoff C, Srivastava M, Cordwell SJ, Geary S, Liao X, Minion FC, Walker MJ, Djordjevic SP: P159 is a proteolytically processed, surface adhesin of Mycoplasma hyopneumoniae: defined domains of P159 bind heparin and promote adherence to eukaryote cells. Mol Microbiol. 2006, 60: 669-686. 10.1111/j.1365-2958.2006.05139.x.
Wilton J, Jenkins C, Cordwell SJ, Falconer L, Minion FC, Oneal DC, Djordjevic MA, Connolly A, Barchia I, Walker MJ, Djordjevic SP: Mhp493 (P216) is a proteolytically processed, cilium and heparin binding protein of Mycoplasma hyopneumoniae. Mol Microbiol. 2009, 71: 566-582. 10.1111/j.1365-2958.2008.06546.x.
Seymour LM, Deutscher AT, Jenkins C, Kuit TA, Falconer L, Minion FC, Crossett B, Padula M, Dixon NE, Djordjevic SP, Walker MJ: A processed multidomain mycoplasma hyopneumoniae adhesin binds fibronectin, plasminogen, and swine respiratory cilia. J Biol Chem. 2010, 285: 33971-33978. 10.1074/jbc.M110.104463.
Deutscher AT, Jenkins C, Minion FC, Seymour LM, Padula MP, Dixon NE, Walker MJ, Djordjevic SP: Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine cilia. Mol Microbiol. 2010, 78: 444-458. 10.1111/j.1365-2958.2010.07345.x.
Seymour LM, Falconer L, Deutscher AT, Minion FC, Padula MP, Dixon NE, Djordjevic SP, Walker MJ: Mhp107 Is a member of the multifunctional adhesin family of Mycoplasma hyopneumoniae. J Biol Chem. 2011, 286 (suppl 12): 10097-10104.
Seymour LM, Jenkins C, Deutscher AT, Raymond BBA, Padula MP, Tacchi JL, Bogema DR, Eamens GJ, Woolley LK, Dixon NE, Walker MJ, Djordjevic SP: Mhp182 (P102) binds fibronectin and contributes to the recruitment of plasmin(ogen) to the Mycoplasma hyopneumoniae cell surface. Cell Microbiol. 2012, 14 (suppl 1): 81-94.
Bogema DR, Deutscher AT, Woolley LK, Seymour LM, Raymond BB, Tacchi JL, Padula MP, Dixon NE, Minion FC, Jenkins C, Walker MJ, Djordjevic SP: Characterization of cleavage events in the multifunctional cilium adhesin Mhp684 (P146) reveals a mechanism by which Mycoplasma hyopneumoniae regulates surface topography. MBio. 2012, 3 (suppl 2): e00282-11.
Zielinski GC, Ross RF: Effect on growth in cell cultures and strain on virulence of Mycoplasma hyopneumoniae for swine. Am J Vet Res. 1990, 51: 344-348.
Pinto P, Klein C, Zaha A, Ferreira H: Comparative proteomic analysis of pathogenic and non-pathogenic strains from the swine pathogen Mycoplasma hyopneumoniae. Proteome Science. 2009, 7: 45-10.1186/1477-5956-7-45.
Castro LAD, Pedroso TR, Kuchiishi SS, Ramenzoni M, Kich JD, Zaha A, Vainstein MH, Ferreira HB: Variable number of tandem aminoacid repeats in adhesion-related CDS products in Mycoplasma hyopneumoniae strains. Vet Microbiol. 2006, 116: 258-269. 10.1016/j.vetmic.2006.04.022.
Yogev D, Watson-McKown R, Rosengarten R, Im J, Wise KS: Increased structural and combinatorial diversity in an extended family of genes encoding Vlp surface proteins of Mycoplasma hyorhinis. J Bacteriol. 1995, 177: 5636-5643.
Citti C, Kim MF, Wise KS: Elongated versions of Vlp surface lipoproteins protect Mycoplasma hyorhinis escape variants from growth-inhibiting host antibodies. Infect Immun. 1997, 65 (suppl 5): 1773-1785.
Citti C, Nouvel LX, Baranowski E: Phase and antigenic variation in mycoplasmas. Future Microbiol. 2010, 5 (suppl 7): 1073-1085.
Hickman AB, Chandler M, Dyda F: Integrating prokaryotes and eukaryotes: DNA transposases in light of structure. Crit Rev Biochem Mol Biol. 2010, 45: 50-69. 10.3109/10409230903505596.
Westberg J, Persson A, Holmberg A, Goesmann A, Lundeberg J, Johansson K-E, Pettersson B, Uhlén M: The genome sequence of mycoplasma mycoides subsp. Mycoides SC type strain PG1T, the causative agent of contagious bovine pleuropneumonia (CBPP). Genome Res. 2004, 14: 221-227. 10.1101/gr.1673304.
Li Y, Zheng H, Liu Y, Jiang Y, Xin J, Chen W, Song Z: The complete genome sequence of mycoplasma bovis strain hubei-1. PLoS One. 2011, 6: e20999-10.1371/journal.pone.0020999.
Peters IR, Helps CR, McAuliffe L, Neimark H, Lappin MR, Gruffydd-Jones TJ, Day MJ, Hoelzle LE, Willi B, Meli M, Hofmann-Lehmann R, Tasker S: RNase P RNA gene (rnpB) phylogeny of Hemoplasmas and other Mycoplasma species. J Clin Microbiol. 2008, 46: 1873-1877. 10.1128/JCM.01859-07.
Volokhov DV, Simonyan V, Davidson MK, Chizhikov VE: RNA polymerase beta subunit (rpoB) gene and the 16S–23S rRNA intergenic transcribed spacer region (ITS) as complementary molecular markers in addition to the 16S rRNA gene for phylogenetic analysis and identification of the species of the family Mycoplasmataceae. Mol Phylogen Evol. 2011, 62: 515-528.
Marinus MG, Casadesus J: Roles of DNA adenine methylation in host- pathogen interactions: mismatch repair, transcriptional regulation, and more. FEMS Microbiol Rev. 2009, 33: 488-503. 10.1111/j.1574-6976.2008.00159.x.
Kumar R, Mukhopadhyay AK, Ghosh P, Rao DN: Comparative transcriptomics of H. pylori strains AM5, SS1, and their hpyAVIBM deletion mutants: possible roles of cytosine methylation. PLoS One. 2012, 7: e42303-10.1371/journal.pone.0042303.
Reisenauer A, Kahng LS, McCollum S, Shapiro L: Bacterial DNA methylation: a cell cycle regulator. J Bacteriol. 1999, 181: 5135-5139.
Wion D, Casadesus J: N6-methyl-adenine: an epigenetic signal for DNA- protein interactions. Nat Rev Microbiol. 2006, 4: 183-192. 10.1038/nrmicro1350.
Fälker S, Schmidt MA, Heusipp G: DNA adenine methylation and bacterial pathogenesis. Int J Med Microbiol. 2007, 297: 1-7.
Schwartz D, Quinn TJ, Thorne PS, Sayeed S, Yi A-K, Krieg AM: CpG motifs in bacterial DNA cause inflammation in the lower respiratory tract. J Clin Invest. 1997, 100: 68-73. 10.1172/JCI119523.
Butler JE, Young ND, Lovley DR: Evolution of electron transfer out of the cell: comparative genomics of six Geobacter genomes. BMC Genomics. 2010, 11: 40-10.1186/1471-2164-11-40.
Rogers MB, Watkins RF, Harper JT, Durnford DG, Gray MW, Keeling PJ: A complex and punctate distribution of three eukaryotic genes derived by lateral gene transfer. BMC Evol Biol. 2007, 7: 89-10.1186/1471-2148-7-89.
Grauvogel C, Brinkmann H, Petersen J: Evolution of the glucose-6-phosphate isomerase: the plasticity of primary metabolism in photosynthetic eukaryotes. Mol Biol Evol. 2007, 24: 1611-1621. 10.1093/molbev/msm075.
Thomas J, Cronan JE: The enigmatic acyl carrier protein phosphodiesterase of Escherichia coli. J Biol Chem. 2005, 280: 34675-34683. 10.1074/jbc.M505736200.
Li WH, Yang J, Gu X: Expression divergence between duplicate genes. Trends Genet. 2005, 21: 602-607. 10.1016/j.tig.2005.08.006.
Thomas PD, Wood V, Mungall CJ, Lewis SE, Blake JA: On the use of gene ontology annotations to assess functional similarity among orthologs and paralogs: a short report. PLoS Comput Biol. 2012, 8: e1002386-10.1371/journal.pcbi.1002386.
Henderson B, Martin A: Bacterial virulence in the moonlight: multitasking bacterial moonlighting proteins Are virulence determinants in infectious disease. Infect Immun. 2011, 79 (suppl 9): 3476-3491.
Chen LL, Chung WC, Lin CP, Kuo CH: Comparative analysis of gene content evolution in phytoplasmas and mycoplasmas. PLoS One. 2012, 7 (suppl 3): e34407-
Xu G, Guo C, Shan H, Kong H: Divergence of duplicate genes in exon-intron structure. Proc Natl Acad Sci. 2012, 109: 1187-1192. 10.1073/pnas.1109047109.
Ochmann H, Lawrence JG, Groisman EA: Lateral gene transfer and the nature of bacterial innovation. Nature. 2000, 405: 299-304. 10.1038/35012500.
Philippe H, Douady CJ: Horizontal gene transfer and phylogenetics. Curr Opin Microbiol. 2003, 6: 498-505. 10.1016/j.mib.2003.09.008.
Pollack JD: The necessity of combining genomic and enzymatic data to infer metabolic function and pathways in the smallest bacteria: amino acid, purine and pyrimidine metabolism in Mollicutes. Front Biosci. 2002, 7: d1762-d1781. 10.2741/pollack.
Matic JN, Wilton JL, Towers RJ, Scarman AL, Minion FC, Walker MJ, Djordjevic SP: The pyruvate dehydrogenase complex of Mycoplasma hyopneumoniae contains a novel lipoyl domain arrangement. Gene. 2003, 319: 99-106.
Pereyre S, Sirand-Pugnet P, Beven L, Charron A, Renaudin H, Barré A, Avenaud P, Jacob D, Couloux A, Barbe V, de Daruvar A, Blanchard A, Bébéar C: Life on arginine for Mycoplasma hominis: clues from its minimal genome and comparison with other human urogenital mycoplasmas. PLoS Genet. 2009, 5 (suppl 10): e1000677-
Alvarez RA, Blaylock MW, Baseman JB: Surface localized glyceraldehyde-3-phosphate dehydrogenase of Mycoplasma genitalium binds mucin. Mol Microbiol. 2003, 48: 1417-1425. 10.1046/j.1365-2958.2003.03518.x.
Hoelzle K, Grimm J, Ritzmann M, Heinritzi K, Torgerson P, Hamburger A, Wittenbrink MM, Hoelzle LE: Detection of antibodies against Mycoplasma suis using recombinant antigens and correlation of serological results to hematological findings. Clin Vaccine Immunol. 2007, 14 (suppl 12): 1616-1622.
Dumke R, Hausner M, Jacobs E: Role of Mycoplasma pneumoniae glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in mediating interactions with the human extracellular matrix. Microbiology. 2011, 157: 2328-2338. 10.1099/mic.0.048298-0.
Schreiner AS, Sokoli A, Felder KM, Wittenbrink MM, Schwarzenbach S, Guhl B, Hoelzle K, Hoelzle LE: The surface-localised a-enolase of Mycoplasma suis is an adhesion protein. Vet Microbiol. 2012, 156: 88-95. 10.1016/j.vetmic.2011.10.010.
Minion FC, Jarvill-Taylor KJ, Billings DE, Tigges E: Membrane-associated nuclease activities in mycoplasmas. J Bacteriol. 1993, 175: 7842-7847.
Schmidt JA, Browning GF, Markham PF: Mycoplasma hyopneumoniae mhp379 is a Ca2 + -dependent, sugar-nonspecific exonuclease exposed on the cell surface. J Bacteriol. 2007, 189: 3414-3424. 10.1128/JB.01835-06.
Bizarro CV, Schuck DC: Purine and pyrimidine nucleotide metabolism in Mollicutes. Genet Mol Biol. 2007, 30: 190-201. 10.1590/S1415-47572007000200005.
Friis NF: Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare a survey. Nordisk Vet Medicin. 1975, 27: 337-339.
Sambrook J, Russell DW: Molecular Cloning a Laboratory Manual. 2001, New York: Cold Spring Harbor Laboratory Press
Carraro DM, Camargo AA, Salim AC, Grivet M, Vasconcelos AT, Simpson AJ: PCR-assisted contig extension: stepwise strategy for bacterial genome closure. Biotechniques. 2003, 34: 626-632.
Almeida LGP, Paixão R, Souza RC, da Costa GC, Barrientos FJA, dos Santos MT, de Almeida DF, Vasconcelos ATR: A system for automated bacterial (genome) integrated annotation-SABIA. Bioinformatics. 2004, 20: 2832-2833. 10.1093/bioinformatics/bth273.
Overbeek R, Fonstein M, D’Souza M, Pusch GD, Maltsev N: The use of gene clusters to infer functional coupling. Proc Natl Acad Sci. 1999, 96: 2896-2901. 10.1073/pnas.96.6.2896.
Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W, Lipman D: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25: 3389-3402. 10.1093/nar/25.17.3389.
Apweiler R, Attwood TK, Bairoch A, Bateman A, Birney E, Biswas M, Bucher P, Cerutti L, Corpet F, Croning MDR, Durbin R, Falquet L, Fleischmann W, Gouzy J, Hermjakob H, Hulo N, Jonassen I, Kahn D, Kanapin A, Karavidopoulou Y, Lopez R, Marx B, Mulder NJ, Oinn TM, Pagni M, Servant F, Sigrist CJA, Zdobnov EM: The InterPro database, an integrated documentation resource for protein families, domains and functional sites. Nucleic Acids Res. 2001, 29: 37-40. 10.1093/nar/29.1.37.
Gardy JL, Spencer C, Wang K, Ester M, Tusnády GE, Simon I, Hua S, deFays K, Lambert C, Nakai K, Brinkman FSL: PSORT-B: improving protein subcellular localization prediction for gram-negative bacteria. Nucleic Acids Res. 2003, 31: 3613-3617. 10.1093/nar/gkg602.
Tatusov R, Fedorova N, Jackson J, Jacobs A, Kiryutin B, Koonin E, Krylov D, Mazumder R, Mekhedov S, Nikolskaya A, Rao BS, Smirnov S, Sverdlov A, Vasudevan S, Wolf Y, Yin J, Natale D: The COG database: an updated version includes eukaryotes. BMC Bioinformatics. 2003, 4: 41-10.1186/1471-2105-4-41.
Saier MH, Tran CV, Barabote RD: TCDB: the transporter classification database for membrane transport protein analyses and information. Nucleic Acids Res. 2006, 34 (suppl 1): D181-D186.
Bairoch A, Apweiler R, Wu CH, Barker WC, Boeckmann B, Ferro S, Gasteiger E, Huang H, Lopez R, Magrane M, Martin MJ, Natale DA, O’Donovan C, Redaschi N, Yeh L-SL: The universal protein resource (UniProt). Nucleic Acids Res. 2005, 33: D154-D159.
Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, Rajandream M-A, Barrell B: Artemis: sequence visualization and annotation. Bioinformatics. 2000, 16: 944-945. 10.1093/bioinformatics/16.10.944.
Yuan Z, Mattick JS, Teasdale RD: SVMtm: support vector machines to predict transmembrane segments. J Comput Chem. 2004, 25: 632-636. 10.1002/jcc.10411.
Krogh A, Larsson B, von Heijne G, Sonnhammer ELL: Predicting transmembrane protein topology with a hidden markov model: application to complete genomes. J Mol Biol. 2001, 305: 567-580. 10.1006/jmbi.2000.4315.
Bernsel A, Viklund H, Falk J, Lindahl E, von Heijne G, Elofsson A: Prediction of membrane-protein topology from first principles. Proc Natl Acad Sci. 2008, 105: 7177-7181. 10.1073/pnas.0711151105.
Yu NY, Wagner JR, Laird MR, Melli G, Rey S, Lo R, Dao P, Sahinalp SC, Ester M, Foster LJ, Brinkman FSL: PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes. Bioinformatics. 2010, 26: 1608-1615. 10.1093/bioinformatics/btq249.
Bagos P, Liakopoulos T, Hamodrakas S: Algorithms for incorporating prior topological information in HMMs: application to transmembrane proteins. BMC Bioinformatics. 2006, 7: 189-10.1186/1471-2105-7-189.
Tatusov RL, Natale DA, Garkavtsev IV, Tatusova TA, Shankavaram UT, Rao BS, Kiryutin B, Galperin MY, Fedorova ND, Koonin EV: The COG database: new developments in phylogenetic classification of proteins from complete genomes. Nucleic Acids Res. 2001, 29: 22-28. 10.1093/nar/29.1.22.
Hall TA: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999, 41: 95-98.
Roure B, Rodriguez-Ezpeleta N, Philippe H: SCaFoS: a tool for Selection. Concatenation and Fusion of Sequences for phylogenomics. BMC Evol Biol. 2007, 7: S2-
Saitou N, Nei M: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987, 4: 406-425.
Lake JA: A rate-independent technique for analysis of nucleic acid sequences: evolutionary parsimony. Mol Biol Evol. 1987, 4: 167-191.
Kumar S, Nei M, Dudley J, Tamura K: MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform. 2008, 9: 299-306. 10.1093/bib/bbn017.
Page RDM: Visualizing Phylogenetic Trees Using TreeView. Current Protocols in Bioinformatics. 2002, John Wiley & Sons, Inc
Papadopoulos JS, Agarwala R: COBALT: constraint-based alignment tool for multiple protein sequences. Bioinformatics. 2007, 23: 1073-1079. 10.1093/bioinformatics/btm076.
Makarenkov V: T-REX: reconstructing and visualizing phylogenetic trees and reticulation networks. Bioinformatics. 2001, 17: 664-668. 10.1093/bioinformatics/17.7.664.
Boc A, Philippe H, Makarenkov V: Inferring and validating horizontal gene transfer events using bipartition dissimilarity. Syst Biol. 2010, 59: 195-211. 10.1093/sysbio/syp103.
This work was supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), the Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) and the Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ). FMS, LR, MMBF and VGV received scholarships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
We thank the research groups from the Southern Network for Genome Analysis that participated in the initial efforts to isolate and sequence M. hyopneumoniae genomes.
The authors declare they have no competing interests.
FMS performed the genomes organization analyses and comparative analyses of genomes, participated in the interpretation of the results and in the writing of the manuscript. CET, FP and MMBF carried out the phylogenomics and phylogenetics analyses and in the interpretation of the results. LGPA and RS carried out the assemblies genomes and participated in the comparative analyses of genomes. VGV, LR and TG participated in the surface proteins in silico analyses. ISS, HBF, ATV and AZ conceived, designed and coordinated the study, participated in the interpretation of the results and in the writing of the manuscript. All authors read and approved the final manuscript.
Electronic supplementary material
Additional file 1: M. flocculare genome.(XLS 96 KB)
Additional file 2: M. hyorhinis genome.(XLS 108 KB)
Additional file 4: M. hyorhinis and M. flocculare genomes in relationship of M. hyopneumoniae OC organization.(XLS 122 KB)
Additional file 6: M. hyopneumoniae genome. 6B. Exclusive OCs of M. flocculare genome. 6C. Conserved Ocs among the three mycoplasmas species. 6D. Exclusive OCs of M. hyorhinis genome. (XLS 76 KB)
Additional file 7: M. flocculare contigs with conservation regions. B. M. flocculare contigs with inversion regions. (XLS 34 KB)
Additional file 8: M. flocculare , M. hyopneumoniae 7448 and M. hyorhinis HUB-1.(PDF 26 KB)
Additional file 9: M. flocculare (A), M. hyopneumoniae 7448 (B) and M. hyorhinis HUB-1 (C).(PDF 97 KB)
Additional file 10: M. flocculare (A), M. hyopneumoniae 7448 (B), and M. hyorhinis HUB-1 (C). B: List of predicted surface proteins of M. flocculare (A), M. hyopneumoniae 7448 (B), and M. hyorhinis HUB-1 (C). List of predicted surface proteins of M. flocculare (A), M. hyopneumoniae 7448 (B), and M. hyorhinis HUB-1 (C). (XLS 237 KB)
Additional file 11: M. flocculare not shared with M. hyopneumoniae 7448 and M. hyorhinis HUB_1. B. List of predicted surface proteins of M. hyopneumoniae 7448 not shared with M. flocculare and M. hyorhinis HUB-1. C. List of predicted surface proteins of M. hyorhinis HUB-1 not shared with M. flocculare and M. hyopneumoniae 7448. (XLS 49 KB)
Additional file 12: M. flocculare , M. hyopneumoniae 7448 and M. hyorhinis HUB-1.(XLS 84 KB)
Additional file 14: M. flocculare , M. hyopneumoniae and M. hyorhynis P97 and P97-like adhesin orthologs and paralogs.(PDF 97 KB)
Additional file 15: The Neighbor-Joining method, using p-distance to compute the evolutionary distances and complete deletion of gaps was implemented by MEGA 5 software. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,500 replicates) are shown next to the branches. S. pyogenes was used as outgroup. (PDF 10 KB)
Additional file 16: The Neighbor-Joining method, using Poisson correction to compute the evolutionary distances and complete deletion of gaps was implemented by MEGA 5 software. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,500 replicates) are shown next to the branches. S. pyogenes was used as outgroup. (PDF 9 KB)
Additional file 17: Evolutionary history of mycoplasmas obtained through a phylogenomic approach. The Neighbor-Joining method was the same description of the Additional file 16. (PDF 8 KB)
Additional file 18: The Maximum Parsimony method using the close-neighbor-interchange algorithm, and the complete deletion of gaps was implemented by MEGA 5 software. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) are shown next to the branches. S. pyogenes was used as outgroup. (PDF 9 KB)
Additional file 19: Evolutionary history of DNA methylases from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method, using p-distance to compute the evolutionary distances and complete deletion of gaps was implemented by MEGA 5 software. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to the branches. (PDF 8 KB)
Additional file 20: Evolutionary history of ATP synthases from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method was the same description of the Additional file 19. (PDF 11 KB)
Additional file 21: Evolutionary history of ribulose-phosphate-3epimerases from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method was the same description of the Additional file 19. (PDF 8 KB)
Additional file 22: Evolutionary history of oligoendopeptidases from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method was the same description of the Additional file 16. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to the branches. (PDF 9 kb) (PDF 9 KB)
Additional file 23: Evolutionary history of single-strand binding proteins from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method was the same description of the Additional file 19. (PDF 8 KB)
Additional file 24: Evolutionary history of fructose-bisphosphate aldolase proteins from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method was the same description of the Additional file 19. (PDF 8 KB)
Additional file 25: Evolutionary history of dihydrolipoamide dehydrogenase proteins from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method was the same description of the Additional file 19. (PDF 9 KB)
Additional file 26: Evolutionary history of glucose-6-phosphate-isomerase proteins from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method was the same description of the Additional file 19. (PDF 7 KB)
Additional file 27: Evolutionary history of lypoate protein ligases from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method was the same description of the Additional file 19. (PDF 8 KB)
Additional file 28: Evolutionary history of acyl carrier phosphodiesterases from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method was the same description of the Additional file 19. (PDF 10 KB)
Additional file 29: Evolutionary history of lactate dehydrogenases from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method was the same description of the Additional file 19. (PDF 8 KB)
Additional file 30: Evolutionary history of membrane nucleases from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method was the same description of the Additional file 19. (PDF 9 KB)
Additional file 31: Evolutionary history of TRSE-like proteins from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method was the same description of the Additional file 19. (PDF 8 KB)
Additional file 32: Evolutionary history of P97 proteins from mycoplasmas obtained through a phylogenetic analysis. The Neighbor-Joining method was the same description of the Additional file 19. (PDF 7 KB)
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Cite this article
Siqueira, F.M., Thompson, C.E., Virginio, V.G. et al. New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis. BMC Genomics 14, 175 (2013). https://doi.org/10.1186/1471-2164-14-175
- Comparative genomics
- Swine respiratory tract