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Table 3 List of the top 20 longest contigs originated from the 454 run of brain-hypophysis-gonad axis tissues of turbot ( S . maximus )

From: A combined strategy involving Sanger and 454 pyrosequencing increases genomic resources to aid in the management of reproduction, disease control and genetic selection in the turbot (Scophthalmus maximus)

Contig number

Length

No. reads

Average coverage per nucleotide

% GC

Annotation

E-value

Accession number

Database

358

5,012

584

33.2

43.4

Cytochrome c oxidase subunit 3

1.00E-127

UniRef90_C7S7B2

Uniref90

4,333

4,486

133

8.3

45.7

Adrenodoxin-like protein mitochondrial

2.00E-60

UniRef90_Q08C57

Uniref90

2,251

4,042

146

10.5

46.1

SSRU rRNA Cavia porcellus

3.00E-13

AAKN02033512

SSU

425

4,010

293

23.3

52.1

Aspartate/tyrosine/aromatic aminotransferase

1.00E-101

YDR111c

COG

1,894

3,992

212

13.6

44.3

unknown

   

2,165

3,964

158

11.0

50.4

Ubiquitin carboxyl-terminal hydrolase

0

UniRef90_B7Z855

Uniref90

762

3,896

464

32.4

48.6

Proliferation-associated 2G4b

1.00E-165

dre:323462

KEGG

1,956

3,797

182

13.8

47.5

Novel protein (Zgc:55794)

0

UniRef90_Q1LWK5

Uniref90

9,828

3,757

54

4.3

44.1

FAM3C

9.00E-57

UniRef90_B5X712

Uniref90

12,120

3,753

63

4.3

47.0

Zgc:165446 protein

1.00E-65

UniRef90_A6H8R7

Uniref90

3,201

3,707

128

10.3

46.6

Novel protein similar to WDR44

0

dre:569045

KEGG

2,212

3,700

116

9.1

38.6

Osteocalcin

2.00E-12

UniRef90_D2XEB2

Uniref90

1,541

3,673

181

14.7

48.3

Cell division cycle

1.00E-105

UniRef90_Q6PFU4

Uniref90

2,167

3,623

173

12.3

45.1

M-phase phosphoprotein 10

1.00E-148

dre:323426

KEGG

280

3,613

287

27.3

44.9

NADH dehydrogenase subunit 5

0

UniRef90_C7S7B6

Uniref90

4,193

3,611

85

6.2

41.3

Suppressor of tumorigenicity 7 protein

1.00E-34

UniRef90_Q1RLU8

Uniref90

644

3,571

531

42.1

46.3

RAD1 homolog

1.00E-145

UniRef90_Q6P2T4

Uniref90

1,248

3,569

127

10.8

43.8

60S ribosomal protein

2.00E-45

UniRef90_P61513

Uniref90

2,845

3,555

106

8.1

43.4

NF-kappaB repressing factor

1.00E-79

gga:422370

KEGG

5,634

3,550

84

5.8

47.1

WD repeat domain phosphoinositide

0

UniRef90_Q5MNZ6

Uniref90