Figure 8

Depletion of miR-101 fails to prevent UVB-induced senescence. Cells were irradiated with UVB twice a day for 4 days. After the last exposure the cells were reverse transfected as described with miR-101 power inhibitors for knockdown, negative control or not treated. Reverse transfection was repeated at day 7. At day 9 RNA and protein were isolated. A. Control cells. Expression levels of miR-101 and Ezh2 mRNA of non-irradiated cells after miR-101 knockdown were determined by real-time qPCR. Bars indicate the mean ± SE of three independent experiments. B. UVB-treated cells miR-101 and Ezh2 expression levels from UVB treated cells after miR-101 knockdown were determined by real-time qPCR. Bars indicate the mean ± SE of three independent experiments. C. Growth curve analysis of miR-101 knockdown cells. cPDLs were calculated as described. Data represent the mean ± SD of three independent experiments. cPDL: cumulative population doublings KD: knockdown. *p < 0.01; **p < 0.001.