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Table 6 DGE sequencing quality evaluation and alignment statistics

From: Transcriptome analysis of Cymbidium sinense and its application to the identification of genes associated with floral development

Summary VP FDP RP
Only adaptor reads 25804 19835 37799
Only adaptor reads% 0.21% 0.16% 0.31%
Containing N reads 37 40 43
Containing N reads% 0.00% 0.00% 0.00%
Low quality reads 25686 32107 16577
Low quality reads% 0.21% 0.25% 0.14%
Total clean reads 11981512 12647899 12164959
Total clean reads% 99.57% 99.59% 99.55%
Total base pairs 587094088 619747051 596082991
Total mapped reads to gene 10795616 11847282 10713910
Total% of mapped reads 90.10% 93.67% 88.07%
Perfect match 7558927 10028451 8649669
Perfect match% 63.09% 79.29% 71.10%
≤2 bp mismatch 3236689 1818831 2064241
≤2 bp mismatch% 27.01% 14.38% 16.97%
Unique match 9389031 10763499 8207609
Unique match% 78.36% 85.10% 67.47%
Multi-position match 1406585 1083783 2506301
Multi-position match% 11.74% 8.57% 20.60%
Total unmapped reads 1185896 800617 1451049
Total unmapped reads% 9.90% 6.33% 11.93%