Table 6 DGE sequencing quality evaluation and alignment statistics
Summary | VP | FDP | RP |
|---|---|---|---|
Only adaptor reads | 25804 | 19835 | 37799 |
Only adaptor reads% | 0.21% | 0.16% | 0.31% |
Containing N reads | 37 | 40 | 43 |
Containing N reads% | 0.00% | 0.00% | 0.00% |
Low quality reads | 25686 | 32107 | 16577 |
Low quality reads% | 0.21% | 0.25% | 0.14% |
Total clean reads | 11981512 | 12647899 | 12164959 |
Total clean reads% | 99.57% | 99.59% | 99.55% |
Total base pairs | 587094088 | 619747051 | 596082991 |
Total mapped reads to gene | 10795616 | 11847282 | 10713910 |
Total% of mapped reads | 90.10% | 93.67% | 88.07% |
Perfect match | 7558927 | 10028451 | 8649669 |
Perfect match% | 63.09% | 79.29% | 71.10% |
≤2 bp mismatch | 3236689 | 1818831 | 2064241 |
≤2 bp mismatch% | 27.01% | 14.38% | 16.97% |
Unique match | 9389031 | 10763499 | 8207609 |
Unique match% | 78.36% | 85.10% | 67.47% |
Multi-position match | 1406585 | 1083783 | 2506301 |
Multi-position match% | 11.74% | 8.57% | 20.60% |
Total unmapped reads | 1185896 | 800617 | 1451049 |
Total unmapped reads% | 9.90% | 6.33% | 11.93% |