Figure 9

Differential expression of KCNJ3 and KCNJ3L in adult male zebra finch brain. (A-E) Photomicrographs of in situ hybridizations for KCNJ3 and KCNJ3L. The approximately locations of the photomicrographs in A-C are depicted in the schematic to the right of panel A. The approximate location for the photomicrographs in panels D and E is similar to that for panel C except in a slightly more medial brain section. (A-C) KCNJ3 is highly expressed through the brain in most major cell types, including ependymal cells in the ventricle (A), cells in the granular, Purkinje, and molecular layers of the cerebellum (B), as well as various neuronal and glia populations in brainstem areas and fiber tracts (C). (D-E) In contrast, KCNJ3L shows relatively low to no expression throughout the brain, but very strong expression in neurons of the AVT, and possibly substantia nigra (D; the dotted rectangle indicates the approximate position of the photomicrograph presented in E). (E) High-power view of KCNJ3L labeled cells in AVT. Scale bars: 100 μm in A-C and E; 1 mm in D. Gene abbreviations are given in Table 1. See Abbreviations for a list of anatomical abbreviations.