Figure 2

Gene interaction network in abdominal fat of LL chickens associated with hemostasis. Functional gene interactions networks were identified by Ingenuity Pathway Analysis (IPA®) software. This network shows direct gene interactions mainly in abdominal fat of LL chickens related to “Hematological System Development and Function” (A). The IPA® Upstream Regulator Analysis identified transcription factors with direct actions on differentially expressed target genes in abdominal fat of FL and LL chickens. This analysis of upstream regulators (based on expected responses from literature and observed responses in the data set) predicts inhibition (blue color) of hepatic nuclear factor 1A (HNF1A) (B) and peroxisome proliferator-activated receptor gamma (PPARG) (C), which would lead to inhibition (blue edges or lines) of target gene expression. Red gene symbols indicate higher expression in the FL and green gene symbols indicate higher expression in the LL.