Figure 2

Expression profiles of select conserved cis -antisense RNAs and cutoRNAs. (A) Expression profile of the antisense gene opposite nuoE and nuoF. (B) Expression profile of a conserved asRNA opposite homologous genes in S. coelicolor, S. avermitilis and S. venezuelae which encompassed the site of ΦBT1 (Top) or ΦC31 (Bottom). The site of phage integration is marked with a purple ‘x’. (C) cutoRNA shared between wblA and a conserved downstream gene (sco3578/sav4585/sven3348). Red graphs (top) represent relative read coverage (long transcript library) for the positive strand (orange is the equivalent for the sRNA-enriched library), while blue graphs represent read coverage (long read library) for the negative strand (green represents the same in the sRNA-enriched library). As expression levels of different genes varied over several orders of magnitude, the y-axis for each gene set was scaled independently. When expression levels differed greatly for the positive and negative strand within a gene pair, the profile of the more highly expressed gene was cut off with a yellow line, to ensure that expression from the less highly expressed gene could be visualized.