cutoRNA expression within secondary metabolic clusters. (A) Expression levels for a cutoRNA pair shared by actVA6 and actR within the actinorhodin cluster of S. coelicolor. (B) Expression profiles for a cutoRNA pair (sco0491 and sco0490) within the coelichelin siderophore biosynthetic cluster of S. coelicolor. (C) Expression of a four gene region within the chloramphenicol biosynthetic cluster of S. venezuelae. Transcription of the cmlDE operon generates an asRNA/cutoRNA to cmlF. (D) Expression profile of a transcriptionally complex region within the avermectin biosynthetic cluster of S. avermitilis. A cutoRNA pair exists between aveR and aveF. Multiple antisense RNAs were found opposite the aveDF operon. For (A-D), relative sequence reads at each nucleotide position were shown in red for the positive strand (top), and blue for the negative strand (bottom). Given the differing expression levels observed for each gene cluster, y-axes for each panel were scaled independently.