Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar)

BMC Genomics

Table 7 Primers used for qPCR

Name¹	Accession²		Sequence 5′ to 3′	bp³
EF-1α	AF498320.1	F	CAAGGATATCCGTCGTGGCA	327
		R	ACAGCGAAACGACCAAGAGG
RPL1	CB516726	F	ACTATGGCTGTCGAGAAGGTGCT	118
		R	TGTACTCGAACAGTCGTGGGTCA
HPRT1	EG866745	F	CCGCCTCAAGAGCTACTGTAAT	255
		R	GTCTGGAACCTCAAACCCTATG
IL-1β	AJ223954.1	F	GCTGGAGAGTGCTGTGGAAGAACATATAG	179
		R	CCTGGAGCATCATGGCGTG
Hepcidin	NM_001140849.1	F	CATTGAAAATCGTGCATTGG	150
		R	AGGCCTTCATTCTCGGTTTT
IGFBP-6	DQ190459.2	F	GCTCAATAGTGTTCTGCGTGG	118
		R	CTTGGAGGAACGACACTGCTT
TNFα1	NM_001123589.1	F	TGTGTGGCGTCCTCTTAGTAGCAGCTT	101
		R	CTCCATTTTGTCCTGCATCGTTGC
IGFBP-4	EU861007	F	TGTCGTGCTGAGCTGCAGAG	129
		R	TGGCTGGCACTGCTTGGCAT
IGFBP-5.2	EU861009	F	TTCTCCAGAGGAAGCTATGTTAG	170
		R	TCAAGGCTGCTGACAGAGTG
Myf5	TC97553	F	CGCATACCGCTTTTACTTCC	245
		R	TGATCATGAGAAACGTGAAGC
High choriolytic enzyme	TC63579	F	ATCAATGGGGCTCATCTCAG	239
		R	ATGAGCAAACACGCAGTGAC
Atrogin-1	GU456729.1	F	CGAGTGCTTCCAGGAGAATCTG	384
		R	GTCTGAAGGAGCTCCTTGATGG

List of primers used in qPCR, all primers were checked to ensure that they had an efficiency of between 1.8 & 2.0. All except for Atrogin 1 were used for qPCR confirmation of the microarray.¹Identity of the gene, ² Accession number of the cDNA sequence, ³Size of PCR product produced by the primers.

ISSN: 1471-2164