Whole genome comparative analysis of channel catfish (Ictalurus punctatus) with four model fish species
© Jiang et al.; licensee BioMed Central Ltd. 2013
Received: 19 May 2013
Accepted: 28 October 2013
Published: 11 November 2013
Comparative mapping is a powerful tool to study evolution of genomes. It allows transfer of genome information from the well-studied model species to non-model species. Catfish is an economically important aquaculture species in United States. A large amount of genome resources have been developed from catfish including genetic linkage maps, physical maps, BAC end sequences (BES), integrated linkage and physical maps using BES-derived markers, physical map contig-specific sequences, and draft genome sequences. Application of such genome resources should allow comparative analysis at the genome scale with several other model fish species.
In this study, we conducted whole genome comparative analysis between channel catfish and four model fish species with fully sequenced genomes, zebrafish, medaka, stickleback and Tetraodon. A total of 517 Mb draft genome sequences of catfish were anchored to its genetic linkage map, which accounted for 62% of the total draft genome sequences. Based on the location of homologous genes, homologous chromosomes were determined among catfish and the four model fish species. A large number of conserved syntenic blocks were identified. Analysis of the syntenic relationships between catfish and the four model fishes supported that the catfish genome is most similar to the genome of zebrafish.
The organization of the catfish genome is similar to that of the four teleost species, zebrafish, medaka, stickleback, and Tetraodon such that homologous chromosomes can be identified. Within each chromosome, extended syntenic blocks were evident, but the conserved syntenies at the chromosome level involve extensive inter-chromosomal and intra-chromosomal rearrangements. This whole genome comparative map should facilitate the whole genome assembly and annotation in catfish, and will be useful for genomic studies of various other fish species.
With the advances of next generation sequencing technology, genomic resources are rapidly expanding, even for non-model species. Among teleost species, whole genomes of five model species have been fully sequenced and assembled, including zebrafish (Danio rerio) (http://www.ensembl.org), fugu (Fugu rubripes) , Tetraodon (Tetraodon nigroviridis) , medaka (Oryzias latipes) [3, 4] and three-spined stickleback (Gasterosteus aculeatus) . Among aquaculture fish species, whole genome reference sequence has been only published for Atlantic cod , although genomes of many aquaculture species have been or are being sequenced. In recent years, great efforts on generating genomic resources have been made for economically important aquaculture species , such as Atlantic salmon [8–11], European sea bass [12–16], tilapia [17–22], rainbow trout [23–28], gilthead sea bream [29, 30], and catfish (for reviews, see [31, 32]). These genomic resources included expressed sequence tags (ESTs), BAC end sequences, physical maps, genetic linkage maps, and radiation hybrid maps.
In the absence of whole genome sequences for most aquaculture species, comparative genomic analysis is useful. Comparative mapping allows identification of evolutionarily conserved chromosomal regions, i.e., conserved syntenies, which facilitate the understanding of genome organization, rearrangement, duplication and evolution [33–37]. Moreover, the conserved syntenies provide physical evidence for orthologies and genome annotation, which is particularly important when dealing with multi-gene families [38, 39]. Comparative genome analysis can also enhance the efficiency for the identification of candidate genes controlling production traits of interest, when coupled with quantitative trait loci (QTL) mapping analysis .
Comparative mapping was initially demonstrated by Fujiyama et al.  for constructing the human-chimpanzee comparative map using chimpanzee BAC end sequences to hit against human genome sequences. Putative orthologues were identified between these two closely related species. Later on, comparative mapping was extensively performed among mammals, such as the construction of human-cattle , human-porcine , human-horse  and human-sheep  comparative maps. High percentage of BLAST hits and/or high level of genome colinearity made the comparative mapping successful . However, whole genome comparative mapping in most teleost species is still limited due to lacking of genomic resources.
Channel catfish, Ictalurus punctatus, is the predominant aquaculture species in the United States. To gain understanding of the catfish genome, considerable efforts have been made toward the development of genomic resources, including genetic linkage maps [46–49], large-insert libraries [50, 51], physical maps [52, 53], BAC end sequences [45, 54], a large number of Sanger sequenced ESTs from various tissues and developmental stages [55–59], full length cDNAs , RNA-Seq transcriptome assemblies [61, 62], and whole genome shotgun sequence reads (unpublished). Such genomic resources provided a foundation for comparative analysis. For instance, Wang et al.  utilized catfish BAC end sequences to compare with zebrafish and Tetraodon genome, and identified conserved synteny regions in the catfish genome. More recently, Liu et al.  conducted comparative mapping analysis by using a large number of BAC end sequences. Genetic linkage map containing type I gene-associated markers was also used for comparative analysis . With next-generation sequencing data, Jiang et al.  conducted comparative analysis between an approximately 1 Mb DNA region in catfish genome with other model fish species. Recently, one catfish linkage group (chromosome) was compared with model fish . These studies allowed for identification of conserved syntenies in the catfish genome as compared with other sequenced fish genomes. In these studies, however, only a small number of gene markers or only one chromosome was used for comparative analysis.
To obtain detailed comparative information at the genome level, whole genome comparative analysis is much needed. We report here the whole genome comparative analysis of catfish with four model fish species, zebrafish, medaka, Tetraodon and stickleback, utilizing all currently available catfish genomic resources. With the whole genome comparative mapping, homologous chromosomes were identified and a large number of conserved syntenies were identified.
Identification of genomic sequences mapped on the catfish linkage map
Summary of statistics of anchored scaffolds
No. of scaffolds
N50 of scaffolds (bp)
Total span (Mb)
No. of the anchored scaffolds
N50 of the anchored scaffolds (bp)
Total length of the anchored scaffolds (Mb)
Summary of genome resources used to anchor genes to catfish linkage groups
BAC-associated markers in linkage map
BAC contigs containing the BAC-associated markers
All BAC-end sequences (BES) from mapped BAC contigs
Physical map contig-specific reads (PMCSS)
Total length of mapped draft genome contigs (Mb)
Unique medaka genes with mapped genome contig hits
Medaka gene hits mapped to chromosomes
Unique Tetraodon genes with mapped genome contig hits
Tetraodon gene hits mapped to chromosomes
Unique stickleback genes with mapped genome contig hits
Stickleback gene hits mapped to chromosomes
Unique zebrafish genes with mapped genome contig hits
Zebrafish gene hits mapped to chromosomes
Identification of homologous genes
The 517 Mb genome sequences retrieved from the draft catfish genome scaffolds were used for further comparative genome analysis. Genes located in these sequences were identified by BLASTX search against ENSEMBL protein database, including protein sequences from zebrafish, medaka, Tetraodon, and stickleback. Homologous genes in these species were identified as summarized in Table 2. The largest number of homologous genes (14,035) was found in zebrafish genome. Of the 14,035 homologous genes, 13,784 genes have chromosome information based on current zebrafish genome annotation in ENSEMBL (Table 2). A total of 9,949 homologous genes were identified in medaka genome. Of which, 9,036 genes were mapped on the chromosomes of medaka genome. Similar numbers of homologous genes were identified from Tetraodon and stickleback genome, with 7,181 and 9,465 being mapped to the chromosomes, respectively (Table 2).
Identification of homologous chromosomes
Eleven catfish linkage groups and zebrafish chromosomes had a one-to-one homologous relationship. These linkage groups included LG4, LG5, LG11, LG13, LG15, LG16, LG17, LG22, LG23, LG25 and LG28. Of all 29 catfish linkage groups, 17 linkage groups were found to be homologous to a single chromosome in zebrafish. Of the 17 catfish linkage groups, five are extremely highly conserved with over 81-91% of their genes shared between the catfish linkage groups and the zebrafish chromosomes. The catfish LG26 and zebrafish chromosome 5 shared 91% of the genes, followed by LG28 sharing 87% genes with zebrafish chromosome 20, LG10 sharing 85% genes with zebrafish chromosome 1, LG15 sharing 82% genes with zebrafish chromosome 23, and LG14 sharing 81% genes with zebrafish chromosome 5 (Figure 2).
Twelve catfish linkage groups were found to be homologous to more than one chromosome in zebrafish. Of which, 10 catfish LGs were homologous to two zebrafish chromosomes each, and two catfish LGs were homologous to three zebrafish chromosomes each (Figure 2). For instance, homologous genes located on catfish linkage group 12 were found in both zebrafish chromosome 6 (38%) and chromosome 19 (37%). Similarly, homologous genes located on catfish linkage group 29 were found in three zebrafish chromosomes: chromosome 1 (39%), chromosome 7 (18%), and chromosome 13 (18%).
When the vast majority of the genes located on one catfish linkage group are homologous to genes located on a single zebrafish chromosome, e.g. catfish LG26 and zebrafish chromosome 5 that share 91% of the genes, it is apparent that these chromosomes are homologous chromosomes. However, when much lower percentage of genes are homologous between a catfish linkage group and a zebrafish chromosome, e.g., around 10%, further analysis is required to provide information as to if chromosomal segments are orthologous with conserved syntenies. Examination of genes and their orders within the catfish scaffolds and zebrafish chromosomes demonstrates that they are indeed syntenic and therefore, likely orthologous. For instance, 27% of genes on catfish LG1 are homologous to genes on zebrafish chromosome 24. On zebrafish chromosomes, these genes were organized in two genomic segments, one spanning approximately 16 Mb from the beginning of the chromosome 24 (position 2,096) to position 16,741,284, and the other spanning approximately 14.5 Mb starting from position 29,305,847 to position 43,867,471 (Additional file 1). In catfish, as the whole genome assembly is not yet available, our analysis is limited to locate multiple genes within a single scaffold, followed by the analysis of the physical map and linkage map positions of the involved scaffolds. As shown in Additional file 1, many genes located in the same zebrafish genomic segments were also located in a single scaffold of the catfish draft genome sequence, and these scaffolds were mapped to similar locations on the linkage map.
Chromosome level conservation was the highest between catfish and zebrafish followed by stickleback, medaka, and Tetraodon. As shown in Figure 2, the one-to-one chromosome relationship was also observed between catfish and medaka, catfish and Tetraodon, and catfish and stickleback, but apparently at a lower level as compared with the situation between catfish and zebrafish. This was reflected at two levels. First, the percentage of homologous genes with a one-to-one relationship was much lower between catfish and medaka, catfish and Tetraodon, and catfish and stickleback as compared to catfish and zebrafish. Second, the level of chromosome rearrangements was much greater between catfish and medaka, catfish and Tetraodon, and catfish and stickleback as compared with catfish and zebrafish (Figure 2). The lowest level of chromosomal conservation was between catfish and Tetraodon.
Identification of conserved syntenic blocks between catfish and zebrafish
Summary of conserved syntenic blocks between catfish linkage groups and zebrafish chromosomes
Total length spanned (Kb)
No. of syntenies
Ave. size of the synteny (Kb)
Max. size of the synteny (Kb)
N50 of the synteny (Kb)
No. of genes involved
Ave. gene number/synteny
Chromosomal level structural conservations
To gain detailed understanding of evolutionary relationship between catfish and zebrafish, a comparative map was constructed between the catfish linkage groups with their homologous chromosomes in zebrafish. Only gene sequences were used for this comparative analysis because gene sequences are more conserved than sequences in intergenic regions. The positions of physical map contigs were determined in the linkage group based on the locations of BES-associated markers. However, the positions and orders of genes within each physical contig cannot be determined because of the incompletely assembled genome sequences. In addition, a number of catfish genes were stacked because of the low resolution provided by the current genetic linkage map.
Similar results were observed in the other 28 catfish linkage groups, with general large scale chromosome level of genome conservation, but with numerous chromosome breaks, shuffling and rearrangements, which are consistent with previous studies [46, 51].
Rapid development of genomic resources in fish species has provided the opportunity for comparative genome analysis, shedding lights on the structure, organization, function and evolution of vertebrate genomes. In this study, we conducted the whole genome comparative analysis of channel catfish, an important aquaculture species, with several model fish species. By comparing with other fully sequenced model fish species including zebrafish, medaka, stickleback, and Tetraodon, homologous chromosomes among these species were determined and a large number of conserved syntenies were identified, providing valuable information for whole genome assembly and annotation in catfish, and for comparative genome analysis of other teleost species.
Comparative map is a powerful tool in genomics studies, especially for non-model species, by transferring the genomic information from well-studied model species. Comparative map not only allows better understanding genome arrangement during evolution, but also benefits the discovery or confirmation of orthologies among species.
Without a well assembled whole genome sequences, comparative genome analysis can be achieved by using various other genomic resources containing information for genome level conservation. Markers with low levels of conservation in evolution have very limited value for comparative genome analysis, and most often only for very closely related species. For instance, when microsatellite markers on linkage maps were used for comparative analysis, a small number of microsatellites could be successfully mapped, indicating relative low levels of conservation of microsatellites which were derived from non-coding regions of the genome . Comparative analysis among different species using gene-derived markers on linkage maps was more effective because genes are well conserved through evolution . Higher resolution of comparative maps can be achieved by using integrated physical and genetic linkage maps with BAC end sequences. For instance, Zhang et al.  conducted comparative analysis of one catfish chromosome (linkage group 8) with four model fish species utilizing catfish linkage map, physical map, BAC end sequences and draft genome sequences. In that work, 287 unique genes were identified, and a number of conserved syntenies were identified. Although that work demonstrated the utilities of linkage maps when integrated with physical maps with BAC end sequences, the ability to establish whole genome comparative map was hindered by the lack of internal BAC sequences. In order to increase the power of comparative genome analysis, we recently developed one additional valuable genome resource, the physical map contig-specific sequences . In this study, we used all the existing genome resources of catfish for whole genome comparative analysis. The ability to identify long conserved syntenic blocks was much enhanced. For instance, with the same linkage group, LG8, as used in Zhang et al. , we were able to identify 585 unique genes, more than the double of the 287 genes identified in the previous study , and the size of syntenic blocks were much increased.
The strategy developed in this study allowed whole genome comparative analysis of catfish conducted without a well-assembled whole genome sequence. However, lacking of a continuous reference sequences, the order and orientation of catfish draft genome contigs/scaffolds within the same catfish physical contig cannot be determined at present. The reason for this inability is the low resolution of the linkage map. Because of the low resolution of the linkage map, many genes are mapped directly or indirectly to the same map location, forming stacks of sequence contigs and scaffolds with gene orders and orientations undetermined. Therefore, it is imperative to develop linkage maps with many more markers or using high density marker such as SNPs in the future, and more importantly with high resolution by using large resource families.
Comparative analysis was conducted based on the similarities between gene sequences in catfish and the homologous genes in the genomes of other model fish species. Only genes were used for analysis in this study because gene sequences are more conserved than intergenic sequences. The largest number of homologous genes was found in zebrafish among four model fish species, with 14,035 genes, followed by stickleback with 10,430 genes, medaka with 9,949 genes and Tetraodon with 9,920 genes. This difference of the homologous genes identified in different model fish species may be resulted from two reasons: First, the quality of the reference genome. For instance, of all annotated 32,574 zebrafish genes, only 1,225 (3.7%) are unmapped onto zebrafish chromosomes or mitochrondria, while in Tetraodon, there are 6,487 (31.5%) of all 20,562 genes cannot be mapped (Ensembl database). Second, the phylogenetic relationship between catfish and these model fish species determined that zebrafish is the most closely species to catfish [63, 69–71].
High levels of chromosomal conservations were observed between catfish and the four fish species. However, due to the difference of chromosome numbers among those fish species, e.g. catfish has 29 chromosomes, while zebrafish has 25 chromosomes, Tetraodon has 21 chromosomes, medaka has 24 chromosomes and stickleback has 21 chromosomes, chromosome breakage or fusion would have occurred during evolution. For instance, 33% of genes identified on catfish LG19 were found to be homologous in zebrafish chr.16, and 46% of genes were found to be homologous in zebrafish chr.24 (Figure 2), suggesting catfish LG19 have been created by fusion of chromosomal segments similar to zebrafish chr.16 and chr.24, or inversely the two zebrafish chromosomes have been created by split of the chromosome similar to catfish LG19. Similar cases can be observed between the comparison of catfish and medaka, catfish and Tetraodon, catfish and stickleback, indicated that chromosomal fusions or splits occurred frequently during the teleost evolution.
Sarropoulou et al.  conducted a comparative study in which the syntenic relationship between six non-model fish species genomes were established by using ESTs and microsatellites sequences. Our study here extended that study by adopting a much larger numbers of genes. For instance, catfish LG15 was identified to be the homologous chromosome of medaka chr.7 (C7), Tetradon chr.9 (T9), and stickleback Grp. XII (SXII), respectively, which indicated that C7, T9 and SXII were homologous chromosomes to one another. This was consistent with the results of Sarropulou et al. . Similarly, catfish LG9 (C9) corresponded to Tetraodon T1, T2 and T13 in this study, while T1 was reported to be homologous chromosome to medaka M10 , which also had the highest percentage homologous gene hits to catfish LG9 in our study.
Because zebrafish is the most closely related model fish to catfish, detailed comparative analyses were conducted between them. Catfish has 29 pairs of chromosomes while zebrafish has 25 pairs of chromosomes. Therefore, some zebrafish chromosomes are expected to be homologous to greater than one chromosome in catfish. This was found with several chromosomes (Figure 2). However, the opposite situation was also found with one catfish chromosome being homologous to several zebrafish chromosomes (Figure 2), suggesting extensive chromosome rearrangements during evolution.
A large number of conserved syntenic blocks between catfish and zebrafish were established. Analysis of the conserved syntenies should greatly benefit genome annotation in catfish. This is particularly true when dealing with large gene families and duplicated genes. As reported by Liu et al. , identities of genes involved in large gene families such as the ABC transporter gene families sometimes cannot be resolved by phylogenetic analysis alone. Syntenic analysis is essential to provide orthologous information for the identification of such genes. The inferred orthologies are important not only for the identification and annotation of genes, but also for functional inference based on orthologies . Apparently, catfish genome is well conserved at the chromosomal level with those in other model fish species. However, local chromosome shuffling and rearrangements are extensive (Figure 3). Our whole genome comparative analysis with four teleost species also indicated extensive inter-chromosomal rearrangements during evolution, consistent with the hypothesis that inter-chromosomal rearrangements were increased after whole genome duplication in the teleost lineage [67, 72].
Whole genome comparative analysis of channel catfish was conducted by utilizing currently available catfish genomic resources including genetic linkage map, physical map, BAC end sequences, physical map contig-specific sequences, and the draft whole genome sequences. Homologous genes and homologous chromosomes of catfish as compared with four fully sequenced fish species were identified based on sequence similarities and arrangements of homologous genes along the chromosomes. Detailed comparative analysis between catfish and zebrafish allowed for the establishment of a large number of conserved syntenies, with some being extended in large sizes. The whole genome comparative analysis should facilitate whole genome sequence assembly and annotation, as well as providing insight into genome evolution.
Anchorage of draft genomic sequences on catfish linkage groups
Various currently available catfish genome resources were utilized in this study, including the genetic linkage map , BAC-derived microsatellite markers , BAC-based physical map , BAC end sequences [45, 54], and draft genome sequences (Unpublished data). As shown in Figure 1, the steps to anchor draft genomic contigs on catfish linkage groups are: 1) Starting with all BAC-derived microsatellite markers on the catfish linkage map; 2) Using the markers to identify BAC end sequences from which they were derived; 3) Using the BAC end sequences to determine the physical map contigs mapped on the linkage groups; 4) Collecting all BAC end sequences and the physical map contig-specific sequences from all mapped physical contigs; 5) Using the assembly of BAC end sequences and physical map contig-specific sequences to search for corresponding whole genome draft sequence contigs and scaffolds using BLAST. All identified draft genome sequences and their respective chromosome (linkage group) locations were used for further comparative analysis.
Identification of homologous genes
Before the gene identification, RepeatMasker (Version 3.2.7, http://www.repeatmasker.org/) was used to mask repetitive-elements within the draft genome sequences. The repeat-masked sequences were then used as query for BLASTX searches against the ENSEMBL protein database of other fully sequenced model fish species, including zebrafish (Danio rerio), medaka (Oryzias latipes), stickleback (Gasterosteus aculeatus) and Tetraodon (Tetraodon nigroviridis), with an E-value cutoff of 1e-10. Gene annotation information was retrieved using BioMart (http://www.ensembl.org/biomart/martview) with ENSEMBL protein ID.
Identification of homologous chromosomes
The genome locations of homologous genes from zebrafish, medaka, stickleback and Tetraodon were obtained by using BioMart with respective ENSEMBL gene IDs. The homologous chromosomes corresponding to each catfish linkage group (chromosome) from zebrafish, medaka, Tetraodon and stickleback were determined as the chromosome with a majority of homologous genes. In cases where significant fractions (more than 10%) of genes were located on several chromosomes, all these chromosomes were determined to contain homologous genomic segments.
Identification of conserved syntenies
Conserved syntenies were defined as preserved co-localization of genes on chromosomes from different species. Conserved syntenies were identified based on the genetic locations of BAC-derived microsatellite markers and their associated genes on the linkage map and the genome positions of homologous genes from other model fish species. In this study, conserved syntenies were established when at least two adjacent genes on the model fish chromosome were found within a single contig of the draft catfish genome or scaffold.
Comparative maps between each catfish linkage group with the homologous chromosome from zebrafish were conducted by using MapChart . The genes within a physical map contig were located on catfish linkage group based on the position of BAC-derived microsatellite markers . The comparative maps were drawn based the gene position on catfish linkage map and the position of their homologous genes from zebrafish chromosomes.
This project was supported by Agriculture and Food Research Initiative Competitive Grants with grant no. 2010-65205-20356 and 2012-67015-19410 from the USDA National Institute of Food and Agriculture (NIFA).
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