Figure 4

Divergence on GR residues from set 3, related to the more canonical ER, promotes displacements on the global network of interactions with the half site, concurrent with the variation at DNA curvature. Brown arrows denote global displacement of the interactions network at GR:GRE (right), these accompanying the variation on the DNA axis curvature (red arrow), compared to ER:ERE (left). Asterisks (right) signs the shortening of the chains at positions 153 and 188, with significant loss of interactions. The black thin arrow (right at the DNA major groove) points to the mutual displacements of the respective side chains from the lysine residues 154 and 160; the first due to the loss of a saline bond to residue 153 (due to the E153G substitution) and the second due to apparent electrostatic repulsion with the first. The atoms of the A157 from ER and of the bulkier V157 from GR (this last packing against nucleotides at the central kink) are depicted as transparent van der Walls spheres.