Gene expression profiling by cDNA-AFLP reveals potential candidate genes for partial resistance of ‘Président Roulin’ against Venturia inaequalis

Table 4 Frequency of co-localization of TDFs from ‘Président Roulin’ with RGAs, QTLs and major apple scab R genes

Mapped	Number^b	Cluster RGA^c(%)	QTL^d(%)	Major R gene^d(%)	QTL/cluster RGA/major gene (%)
TDFs^a	387	22	21	4**	40
TDFs group I	130	24	17	5***	38
TDFs group IIa	110	22	24	4	38
TDFs group IIb	147	22	22	5**	44
Uninfected apple library AELA	501	20	21	1	38

^aTranscript-derived fragments (TDFs) at 48 hours after inoculation by V. inaequalis: genotype-specific TDFs (group I), pathogen-responsive TDFs expressed in common by both genotypes (group IIa) and pathogen-responsive and genotype specific TDFs (group IIb). Frequencies of co-localization of TDFs were compared to frequencies observed with an uninfected apple library AELA [34] using a χ² test. P values are indicated as follows: ***= P <0.001, **= 0.001 < P <0.01, and *= 0.01 < P <0.05.
^bNumber of cDNA mapping onto the apple chromosome with a threshold E-value < 0.001 by BLAST analysis against homologous apple genomic sequences with known chromosomal locations [104].
^cclusters of resistance gene analogues (RGAs) [94].
^dQuantitative trait loci (QTLs) and major R genes for apple scab resistance [3, 13, 14].

ISSN: 1471-2164