Genome-wide microarray analysis of Atlantic cod (Gadus morhua) oocyte and embryo

Škugor, Adrijana; Krasnov, Aleksei; Andersen, Øivind

doi:10.1186/1471-2164-15-594

BMC Genomics

Table 2 Primer list for real-time qPCR

From: Genome-wide microarray analysis of Atlantic cod (Gadus morhua) oocyte and embryo

Gene name	Gene symbol	Sequence (5′- 3′)	Product length, bp
nanor	nanor	ATCCAATACCCAACGGTTCA	92
nanor	nanor	GCGATGAAATGGCTGAATCT	92
Heat shock 70 kDa protein 4	hsp70	TGAACAGCGCTATGAACCAG	117
Heat shock 70 kDa protein 4	hsp70	TCATGATGGGGTTACAAGCA	117
Heat shock 90 kDa beta	hsp90ba	CGAGGAGCACTACAACGACA	181
Heat shock 90 kDa beta	hsp90ba	GTCCTGCTTCTCCTTCATGC	181
Stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing)	stip1	CCGATGTCCTGAAGAGGTGT	141
Stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing)	stip1	TCATGGCTAAGGGGTAGTCG	141
Follistatin	fst	ACCTGGAAAGGACCAGTGTG	117
Follistatin	fst	GCACTTTCCCTGGTACTGGA	117
Formin-binding protein 4	fnbp4	GCCTGACCTCCACAGATGTT	100
Formin-binding protein 4	fnbp4	CAACACGGACATCTTCATCG	100
Keratin 12	krt12	GCCAAGACTGACCTGACCAT	188
Keratin 12	krt12	GCCTCGTAGTGTTCCCTGAC	188
Ikaros	ikzf	ATGATCTCCGGGTCTGTGAG	165
Ikaros	ikzf	ACACTTGAGCTTTCCGTCGT	165
Reference genes
ATP synthase subunit s mitochondrial	ATP5s	AACAGGGTGGACTATGAGAGGA	114
ATP synthase subunit s mitochondrial	ATP5s	GTGATGCCAGCGTTCAAA	114
Eukaryotic translation initiation factor 3 subunit 3 gamma 40 kDa isoform CRA b	eIF3	AGGACGACGCAGACTTTGAC	121
	eIF3	ACGAAGGAGCCGTAGAAGGT	121
Tetratricopeptide repeat protein 39C	tpr39	GAAACGGGCTGAGAGACTGA	63
Tetratricopeptide repeat protein 39C	tpr39	ATGACACCCAGGAAGCAGAG	63
Dehydrogenase/reductase SDR family member 11	dhrs11	GGAGACAGAGTTTGCGTTCC	128
Dehydrogenase/reductase SDR family member 11	dhrs11	GAGGGGCACTGAGGACATAA	128
Ubiquitin	ubi	GGCCGCAAAGATGCAGAT	69
Ubiquitin	ubi	CTGGGCTCGACCTCAAGAGT	69

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ISSN: 1471-2164

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