Figure 5

Schematic of the regulation of TOR signalling and S6K activation in O. tauri . Numbered connectors indicate the order of the stages of S6K activation, as proposed by Shin et al.[48]. (1) GSK3 phosphorylates S398 allowing the (2) phosphorylation of T415 by the TORC1 complex. (3) Phosphorylated T415 is able to bind PDK1, which phosphorylates S260, leading to the activation of S6K. Additionally, TOR mediated inhibition of S6K is shown, which may occur via TAP42, previously unannotated in the O. tauri genome. TAP42 in yeast binds PP2A and prevents the latter from dephosphorylating the GSK3 target site of S6K.