Extreme specificity of NCR gene expression in Medicago truncatula
- Ibtissem Guefrachi†1, 2,
- Marianna Nagymihaly†1, 3,
- Catalina I Pislariu†4,
- Willem Van de Velde1, 6,
- Pascal Ratet1,
- Mohamed Mars2,
- Michael K Udvardi4,
- Eva Kondorosi1, 3,
- Peter Mergaert1Email author and
- Benoît Alunni1, 5
© Guefrachi et al.; licensee BioMed Central Ltd. 2014
Received: 19 May 2014
Accepted: 12 August 2014
Published: 25 August 2014
Legumes form root nodules to house nitrogen fixing bacteria of the rhizobium family. The rhizobia are located intracellularly in the symbiotic nodule cells. In the legume Medicago truncatula these cells produce high amounts of Nodule-specific Cysteine-Rich (NCR) peptides which induce differentiation of the rhizobia into enlarged, polyploid and non-cultivable bacterial cells. NCRs are similar to innate immunity antimicrobial peptides. The NCR gene family is extremely large in Medicago with about 600 genes.
Here we used the Medicago truncatula Gene Expression Atlas (MtGEA) and other published microarray data to analyze the expression of 334 NCR genes in 267 different experimental conditions. We find that all but five of these genes are expressed in nodules but in no other plant organ or in response to any other biotic interaction or abiotic stress tested. During symbiosis, none of the genes are induced by Nod factors. The NCR genes are activated in successive waves during nodule organogenesis, correlated with bacterial infection of the nodule cells and with a specific spatial localization of their transcripts from the apical to the proximal nodule zones. However, NCR expression is not associated with nodule senescence. According to their Shannon entropy, a measure expressing tissue specificity of gene expression, the NCR genes are among the most specifically expressed genes in M. truncatula. Moreover, when activated in nodules, their expression level is among the highest of all genes.
Together, these data show that the NCR gene expression is subject to an extreme tight regulation and is only activated during nodule organogenesis in the polyploid symbiotic cells.
KeywordsSymbiosis Legume nitrogen fixation Nodulation Bacteroid Medicago truncatula Sinorhizobium meliloti NCR Defensin Gene expression Transcriptome compendium
Legume plants establish a symbiotic relationship with nitrogen fixing soil bacteria, known as rhizobia. For the purpose of this symbiosis, the plant host forms new, specific organs on its roots called nodules, inside which the symbiotic rhizobia are housed, fix nitrogen (i.e. the enzymatic reduction of nitrogen gas to ammonium) and transfer the ammonium to the plant. Nodules contain several thousand endoreduplicated giant symbiotic cells, which are each infected with thousands of intracellular rhizobia. These symbiotic cells are adapted to the symbiosis, to the metabolic exchange with the nitrogen fixing rhizobia and to the intracellular accommodation of this large bacterial population. The symbiotic cells originate from dividing progenitor cells in the nodule meristem. A key step in the differentiation of the symbiotic cells is the exit of the cell division cycle of nodule meristematic cells and a switch to an endoreduplication cycle in these post-meristematic cells. An endoreduplication cycle is a modified cell cycle with repeated replication of the genome without mitosis and cytokinesis, resulting in polyploid cells with increased DNA content and cell volume. The cell cycle switch is under the control of the Anaphase Promoting Complex (APC) and its activator Ccs52A [1, 2]. The differentiating symbiotic cells are gradually infected and filled with rhizobia. Bacteria are released in the host cells through an endocytosis-like process releasing the intracellular bacteria in organelle-like structures called symbiosomes. Mature symbiotic cells have about 80-fold larger cell volume and endoploidy levels up to 64C compared to the diploid (2C) progenitor cells. Their cytosolic space is entirely packed with symbiosomes and their physiology is adapted for symbiosis, feeding the microsymbionts and assimilating and transporting the fixed nitrogen.
Remarkably, Wildermuth  noticed, by comparing different biotrophic interactions of plants, that host cell polyploidy is a common feature of symbiotic interactions with rhizobium bacteria and arbuscular mycorrhizal fungi, as well as parasitic interactions with fungi and nematodes. Even in symbiotic interactions of insects with endosymbiotic bacteria, host cells that house the endosymbionts are endoreduplicated cells (e.g. ). Thus polyploid host cells may be a well suited adaptation as an interaction site for nutrient exchange with symbiotic microorganisms and some parasites may have evolved to exploit this.
Synchronously with the differentiation of their host cells, the symbiosome bacteria differentiate into nitrogen-fixing bacteria called bacteroids. These bacteroids have a specific physiology and metabolism adapted to the symbiotic life and nitrogen fixation which are dramatically different from those of a free-living bacterium . Interestingly, the differentiation of bacteroids is often accompanied by a morphological and cytological metamorphosis whereby the bacteroid cell becomes enlarged, its envelope fragilized and its genome amplified (polyploid) and condensed [6, 7]. In Medicago truncatula, a class of peptides named NCRs (Nodule-specific Cysteine-Rich Peptides) controls the bacteroid elongation and polyploidization . The NCR peptides are produced by the infected symbiotic cells and are transported to the bacteroid-containing symbiosomes. The NCR peptides can induce bacterial elongation and polyploidization in vitro on cultured rhizobium or in planta when expressed in transgenic Lotus japonicus plants which lack NCRs and form non-elongated bacteroids without genome amplification . Some NCRs accumulate to a significant extent in the cytosol of mature bacteroids  suggesting that these peptides may have additional functions, other than inducing the morphological transformation and notably, it has been suggested that these intracellular NCRs may affect the bacteroid metabolism . Indeed, it has been demonstrated for the peptide NCR247 that it has multiple bacterial targets leading to inhibition of cell division and affecting the bacterial transcriptome and translation that collectively contribute to the altered physiology of the endosymbionts [9–11].
NCRs are similar to the defensin-type of antimicrobial peptides, and some NCR peptides have antimicrobial activity, killing rhizobium and other bacteria when applied at high concentration [8, 9, 12, 13]. Defensins and other types of antimicrobial peptides are found in all eukaryotes where they are part of the first line of defense against invading microbes. Thus the NCR peptides likely evolved from the ancestral immune repertoire. NCR genes were originally thought to be unique to the IRLC legume clade . The bacteroids in the nodules of the tested species of this clade all share the elongation and polyploidization feature . However, refined bioinformatics tools for the prediction of small peptides in genome sequences led to the discovery of three putative Arabidopsis genes that encode peptides with the typical pattern of cysteine residues of the NCRs . The existence of multiple NCR genes in several species of the IRLC clade suggests that the ancestral genes may have gained a new function in symbiosis in the common ancestor of IRLC and that increasing its copy number through gene duplications may have conferred a selective advantage. To counteract the antimicrobial activity of the NCR peptides, Sinorhizobium meliloti, the symbiont of Medicago, requires the BacA protein. In the absence of this protein, the bacteroids are immediately killed by the NCR peptides as soon as they are released in the symbiosomes in nodule cells .
A striking and unusual feature of the NCR gene family in M. truncatula is that it is composed of about 600 genes which are seemingly all expressed in the nodules [14–18]. In situ expression analysis of individual NCR genes or microarray analysis of a large subset of the family has demonstrated that they are all expressed in the symbiotic nodule cells [8, 14, 18, 19]. Moreover, EST analysis and microarray experiments, testing a number of different plant organs as well as different growth conditions, revealed NCR gene expression only in nodules [14, 18, 19].
The Medicago truncatula Gene Expression Atlas (MtGEA) [20, 21] was generated with the whole genome Affymetrix Medicago Gene Chip and compiles microarray data for the majority of M. truncatula genes (50,900 probe-sets) over a large set of experiments (254 different experiments in MtGEA version 3). The compendium is a unique and currently the richest resource for analysing gene expression in M. truncatula. In this study, we used the MtGEA compendium and additional unpublished and published microarray experiments [22, 23] to describe in great detail the expression profiles of the majority of the M. truncatula NCR genes. We show that this gene family has an extreme tissue specific expression profile with undetectable expression in all tissues and conditions except in nodules where they become transcriptionally active to very high levels. In addition, promoter-GUS plants were produced for three NCR genes as well as a specific antibody for one NCR peptide. These tools were used to confirm expression pattern specificity in various conditions, most particularly during biotic interactions.
Global analysis of NCR gene expression
The expression profiles of individual NCR genes show expression to very high levels in the nodule conditions and only background levels in the other experiments (Additional file 2: Figure S2A,B). Such profiles are typical for nearly all NCR genes (Additional file 1: Table S1) but by surveying all NCR probe-sets, 5 exceptions were discovered with more or less relaxed nodule specific expression patterns (Figure 1, green arrowheads; Additional file 2: Figure S2C-G). NCR247 and NCR077 are still mainly expressed in nodules but are also weakly active in other conditions. The NCR247 gene seems to be expressed in different root samples and in some samples from aerial tissues although at lower levels than in nodules. It is not evident from the available information of the different experiments to determine what may activate this expression. NCR077 has a higher than usual background level, possibly because of a less specific probe set, but the gene seems to be also expressed in some mycorrhizal samples (Additional file 2: Figure S2D) including a laser-capture microdissection (LCM) sample of arbuscule-containing cells . NCR218 and NCR122 on the other hand have a completely relaxed specificity and are expressed to similar levels in nodules and in other conditions, mostly roots (Additional file 2: Figure S2E,F). NCR235 expression is similarly specific to most other NCR genes except for a weak expression in stems and shoots, which is about 10- to 100-fold lower than in nodules (Additional file 2: Figure S2G). Also NCR247 is expressed in some of the stem samples. Thus, except for these 5 genes, the complete tested NCR gene set is only expressed in nodules and in none of the other conditions that are present in MtGEA.
Spatio-temporal expression of NCR genes in nodules
When matching the spatial patterns with the corresponding temporal patterns, a fairly good correspondence can be observed: genes expressed in the apex are mostly also fully activated early during the nodule development at 4 or 6 dpi, while genes expressed in the more proximal tissues are activated late in nodule development (Figure 3). The correspondence between the spatial and temporal pattern of NCR gene expression is also obvious when considering the clusters of genes representing the major expression profiles (Additional file 2: Figure S4). For example, genes of clusters 1 and 2 (Additional file 2: Figure S4) are expressed in the most apical part of the nodule and this correlates with an early activation at 4 or 6 dpi in the temporal pattern. On the other hand, genes in cluster 5 have maximum expression only in sample II-III and this corresponds with an activation late in nodule development at 10 dpi (Additional file 2: Figure S4).
Together these spatio-temporal patterns reveal that the NCR genes are activated in different waves, in agreement with our previous results that identified two key points in nodule development associated with major transcriptional activation, one at the formation of symbiotic cells and another one when bacteroids differentiate . Nevertheless, the present analysis is refining this description and shows that NCR genes are activated in at least 3 waves and moreover they can be distinguished by the maintenance or the decline of their expression in the older nodule cells.
NCR genes are not directly involved in nodule senescence
Promoter-GUS analysis and immunolocalization of selected NCRs in nodules
In order to confirm the expression data from MtGEA, stable transgenic M. truncatula R108 lines were generated carrying promoter-GUS fusion constructs for 3 different NCR genes, representing different temporal classes of NCRs and inoculated with Sinorhizobium meliloti strain 1021 or Sinorhizobium arboris strain B554. NCR001 is not activated before the late stages of the nodule formation, NCR084 is slightly induced in early time points (4 dpi) and fully activated at the mature stage of the nodule and finally NCR121 is an early gene which is already fully activated at 4 dpi. GUS expression in the 3 transgenic lines was not detected in root tips or other root parts (Additional file 2: Figure S6). In agreement with its temporal regulation during nodulation, NCR121 expression was induced in young nodule primordia as early as 5dpi and remained expressed throughout the experiment in the entire infection zone and the fixation zone of mature nodules (Additional file 2: Figure S6). NCR084 expression was detected from 11 dpi on and was mainly confined to the proximal infection zone, the interzone II-III and to the distal part of the fixation zone (Additional file 2: Figure S6). NCR001 expression was detectable from 11 dpi in the developing fixation zone and its expression extends in the following days as the fixation zone is growing (Additional file 2: Figure S6). All 3 genes are only expressed in the symbiotic nodule cells. In older nodules, at 30 dpi, displaying a senescence zone, NCR expression was never detected in the senescing tissues, nor was their expression enhanced in the proximal fixation zone adjacent to the senescent tissue (Additional file 2: Figure S6), confirming that NCR genes are not involved in the senescence process. Overall, the temporal and spatial promoter-GUS expression patterns are in very good agreement with the expression profiles deduced from the transcriptome compendium.
The particular expression pattern of NCR122 with its relaxed tissue specificity (Additional file 2: Figure S2F) and its apparent expression in the uninfected nodule cells, together with the availability of an anti-NCR122 antibody prompted us to specifically analyze the localization of the NCR122 peptide in nodules. Immunolocalization of the peptide revealed indeed a specific presence of NCR122 in the uninfected cells of the central zone of a mature nodule as well as in the uninfected cortical cells of the nodule (Additional file 2: Figure S7). Together with the transcriptome data, this indicates that NCR122 and most likely also NCR218 are the only NCR peptides that are specific to uninfected root and nodule cells.
Expression of NCR genes in plant organs
Previously, NCR expression in conditions other than the symbiosis with rhizobium was tested by EST analysis  and with dedicated microarrays , indicating the absence of expression. The MtGEA database offers the possibility to extend this analysis to more plant organs and biotic and abiotic stress conditions.
Besides nodules, 8 other plant organs  were interrogated for NCR expression (Additional file 2: Figure S8) as well as plant treatments with the phytohormones auxin, cytokinin and auxin transport inhibitors [32, 33] (Additional file 2: Figure S9). Interestingly, treatment of roots with the auxin transport inhibitors TIBA or NPA leads to the formation of uninfected nodule-like structures . However, in none of these conditions were NCR genes expressed except for the NCR genes with relaxed expression described above.
NCRs resemble the defensin-type of antimicrobial peptides and plant defensins are often expressed to high levels in “infection-sensitive” organs like flowers or seeds. Because of the complete lack of detectable expression of NCRs in these organs (Additional file 2: Figure S8), they most probably do not have a defensive function in these organs. Nevertheless, many non-NCR defensin-like genes were found to be expressed in seeds, potentially involved in their protection .
Expression of NCR genes after biotic and abiotic stress
Defensins are also induced during infection with pathogens or during salt and drought stresses [34–37]. Therefore, we specifically analyzed how the NCR gene family is expressed during such conditions (Additional file 2: Figure S10 and Additional file 2: Figure S11) [22, 38–45]. Not considering the 5 NCR genes with relaxed specificity, we could not detect expression of any of the NCR genes in all these data sets together (with the possible exception of giant cells formed by the nematode Meloidogyne incognita; Additional file 2: Figure S10B). Several NCRs showed a hybridization signal in giant cells although the level was about 1 to 2 orders of magnitude lower than the signal in nodules for the same NCR gene. However, it should be noted that the giant cells were isolated by LCM and that the array hybridization was performed with an amplified cDNA sample  which could be a source of background hybridization. In any case, besides the possible exception of the giant cells, the data indicate that the NCR genes seem not to be used by the plant to control infections other than the rhizobium bacteria in nodules.
Promoter-GUS analysis of NCR expression during pathogenic interactions
We used the 3 NCR promoter-GUS reporter lines to confirm the absence of NCR expression during pathogenic responses and to complement these observations. The MtGEA dataset includes M. truncatula responses to root pathogens and therefore we analyzed leaf or stem pathogens that encompass also other trophic interactions and infection strategies. Inoculation of M. truncatula leaflets with the necrotrophic soft rotting bacterium Dickeya dadantii 3937 induced maceration symptoms from 1 dpi on, but failed to induce NCR expression (Additional file 2: Figure S12). Similarly infiltration of the virulent strain Pseudomonas syringae pv. tomato DC3000 (Pst) induced necrosis in the infiltrated zone within 2 dpi, whereas the hrcC mutant strain that is unable to form a functional type three secretion system (TTSS) did not induce any visible reaction (Additional file 2: Figure S12). NCR expression was not detected in the infiltrated leaflets in either condition (Additional file 2: Figure S12). Although Pst DC3000 is not described as a natural pathogen of M. truncatula, the necrosis induced by the wild type strain and its absence in the presence of the TTSS mutant suggest that at least some bacterial effector proteins can be specifically recognized by the plant resulting in a necrotic response similar to the hypersensitive response it provokes on non-host Nicotiana benthamiana plants . Similarly, inoculation of the same M. truncatula lines with the necrotrophic polyphagous grey mold-causing fungus Botrytis cinerea yielded typical symptoms at 7 dpi without inducing any detectable NCR expression (Additional file 2: Figure S12). The results of our pathoassays are also in line with a recent study showing that NCR expression was not detected during the compatible interaction of M. truncatula with the hemibiotrophic leaf pathogen Colletotrichum trifolii or with the biotrophic soil pathogen Phytophtora medicaginis. Altogether, our data and the study from  are in agreement with the MtGEA dataset and broaden the conclusion that NCRs are not involved in pathogen responses, whatever the trophic (bio-, hemibio- or necrotrophic) interaction, the host or non-host status and the output of the interaction (disease or resistance). Finally, as herbivory and more generally wounding may induce plant defenses around the wounded zone, we also tested the effect of mechanical wounding on NCR expression but again no NCR expression could be detected in the wounded leaflet (Additional file 2: Figure S12).
NCR genes have very high tissue specificity as measured by Shannon entropy
The above analyses reveal an extreme specificity in expression for the NCR gene family: the genes are only expressed in nodules and not in any other organ or physiological condition. To express this specificity quantitatively and to compare it to other types of specifically expressed genes, the complete MtGEA probe-set was analyzed and their Shannon entropy was calculated. Shannon entropy is a metric for characterizing the uniformity of the expression pattern of a gene over the tested conditions . Low entropy values indicate high tissue specificity while high entropy levels characterize ubiquitous expression.
Although the NCR family is by far the most represented among the nodule-specific low entropy genes, many other known nodule specific genes have very low entropy (Additional file 3: Table S2). These include for example leghemoglobin genes, the Glycine-Rich Peptide (GRPs) genes [16, 49], the Small Nodulin Acidic RNA-binding Protein (SNARP) gene family , genes encoding a small family of secretory calmodulin-like proteins [14, 51], the DNF2 gene involved in suppression of defense responses in the symbiotic cells  and others. Most interestingly, also putative retrotransposons (probe-sets Mtr.9294.1.S1_at and Mtr.636.1.S1_at) and a Dicer 1-like ribonuclease III gene (probe-set Mtr.41531.1.S1_at) are among the nodule specific low entropy genes (Additional file 3: Table S2).
The expression of NCR genes has been studied - in M. truncatula mostly but also in some other IRLC legumes - at the level of individual genes by RT-PCR, in situ hybridization, immuno-localization and promoter-marker gene fusions or at the family level by EST-analysis, macroarrays, dedicated microarrays or whole-genome microarrays [8, 10, 14, 16, 18, 19, 31, 53]. These studies detected NCR expression only in nodules and in no other tested tissues. By mining publicly available whole-genome transcriptome data, we have extended this analysis of NCR gene expression to a very large number of conditions, together covering most plant organs as well as different growth conditions including biotic and abiotic stresses. As a whole, our study suggests that, apart from 5 genes, all NCRs are only expressed in nodules. Moreover, quantifying the specificity of expression with the Shannon entropy factor reveals that the NCR genes, and more generally, nodule specific genes are among the most specifically expressed genes in M. truncatula. This suggests thus that nodulation in Medicago is in large part depending on genes solely dedicated to this symbiotic process. These genes may be resulting from gene duplications followed by neo-functionalization (for example the DNF2 protein or the leghemoglobin proteins which have non-symbiotic homologues) or they may be unique for the symbiosis (possibly the NCRs, GRPs, SNARPs and others). In addition to that, the expression of the NCR genes in nodules reaches very high levels. Even if certain NCR genes are expressed at a low level, the majority of them are among the highest expressed genes in the whole genome of Medicago. This is in agreement with our previous estimation, based on EST counts, that all NCR mRNAs together constitute almost 5% of the total mRNA population in nodules .
In accordance with their resemblance to antimicrobial peptides of the innate immunity such as defensins, many NCR peptides, in particular the most cationic ones, have a strong antimicrobial activity against a diversity of bacteria as well as fungi [8–10; unpublished data]. Despite this, the NCR genes are not expressed in any of the pathogenic interactions of Medicago tested here or by . This included interactions with bacteria, fungi, oomycetes and nematodes. They are also not expressed in organs like leaves, seeds and flowers which often express high levels of innate immunity antimicrobial peptides . Therefore, it seems that the NCR peptides have no function in innate immunity.
In situ detection of NCR expression has demonstrated for all the tested genes that they are specifically expressed in the symbiotic nodule cells but different subsets of NCR genes are activated at different stages of differentiation of these host cells ([8, 10, 14]; this work). Transcriptome analysis extended this pattern to the whole family. The NCR genes are not activated by Nod factors or during the very early stages of the nodule organogenesis when infected cells are not yet formed (this work; [18, 19]). During the development of wild type nodules, they are activated in consecutive waves and their first appearance coincides with the formation of infected symbiotic cells . We show here that NCR genes are activated during nodule development in at least 3 temporal waves corresponding to specific spatial expression patterns. Genes activated early in nodule development are expressed in the more distal nodule parts (close to the apex) while genes activated late during development are expressed in the proximal nodule tissues. In addition, certain clusters of genes, once activated, maintain their activity when the tissues grow older while other clusters are characterized by a decline of their expression in the older nodule cells. Our spatial analysis of NCR expression is in strong agreement with a recently published study  that used LCM of nodule zones coupled to RNA-Sequencing (Additional file 2: Figure S13).
Transcriptome analysis of non-functional nodules that are formed by bacterial or plant symbiotic mutants and that are arrested at different stages of nodule development, is also in agreement with specific expression of all NCR genes in the symbiotic nodule cells: their transcriptional activation is only observed when polyploid symbiotic cells are formed in the mutant nodules . For example, in nodules of the M. truncatula TE7 mutant which is affected in the IPD3 gene [55, 56] and in nodules infected by the S. meliloti exoY mutant, no infected and polyploid symbiotic cells are formed and these nodules do not express any of the NCR genes . Conversely, in nodules infected by the S. meliloti bacA mutant which contain symbiotic cells with undifferentiated bacteroids, only a subset of NCR genes is activated while in other mutants, forming normal symbiotic cells with differentiated bacteroids, NCR genes are activated to a similar extend as in the wild type [18, 19]. Together, the expression pattern of all the tested NCR genes suggests that the endosymbiotic rhizobia in the symbiotic nodule cells are the only targets of the peptides. However, the distinct spatio-temporal profiles clearly suggest that NCR peptides have many different roles. Subsets of NCR genes that are expressed during the early stages of symbiotic cell formation might be involved in the elongation and polyploidization of the bacteroids while other subsets that are active in later stages of symbiotic cell formation or even after the completion of the symbiotic cell differentiation might have other functions in the bacteroids. Moreover, we find that the expression of the NCRs has been shut down when nodule senescence is activated, meaning that the antimicrobial NCR peptides have no direct role in the lysis and digestion of the bacteroids that is taking place during senescence of nodules.
Very little is known about how the very specific regulation of NCRs is achieved. Since their expression is correlated with bacterial infection of the symbiotic cells, the perception of bacterial signals such as components of the bacterial envelope could be involved. The transcription factor EFD, belonging to the ethylene response factor family, may control, directly or indirectly, the expression of a subset of NCR genes since a mutant forms nodules in which part of the NCR genes are downregulated and in which bacteroid differentiation is partially impaired . The IPD3 protein is another transcription factor that might be involved, directly or more likely indirectly, in the regulation of the NCR genes and the other symbiotic cell specific genes [58, 59]. Indeed, in the M. truncatula ipd3 mutant nodules, the symbiotic cells do not form and the symbiotic cell specific genes, including the NCRs, are not activated . In agreement with this, the IPD3 gene is expressed in the whole nodule and its expression domain overlaps with the NCR expression zone .
Searching for potential cis-elements in the promoters of NCR genes with different algorithms yielded 5 different conserved motifs of 41 to 50 bp, which are specifically enriched in the 1000 bp promoter regions . Some of these motifs show resemblance to previously described motifs conferring nodule-specific gene expression. However, the role, if any, of these motifs in the remarkable expression pattern of the NCR genes needs further investigation. Interestingly, some of these motifs comprise Auxin Response Factor binding sites that may suggest a role for auxin in NCR regulation . However, the MtGEA dataset from auxin treated seedlings do not show any NCR induction, pointing out a more complex regulatory mechanism controlling NCR expression.
The very tight regulation of the NCR genes that was revealed here might indicate that besides cis- and trans-acting factors, regulation at the level of chromatin might also be involved in the activation of the NCR genes. Moreover, endoreduplication seems to be a prerequisite for their activation  and might thus, by a presently unknown mechanism, be implicated in the activation of this gene family. In that respect, it is interesting to note that the giant feeding cells induced by the nematode M. incognita are highly polyploid cells and possibly express faintly a few NCR genes. Nevertheless, this observation could be an experimental artefact and will require further experimental confirmation.
Genes with high tissue-specific expression are often actively silenced during most of the plant growth by epigenetic mechanisms. Since in M. truncatula the nodule-specific genes display the highest level of expression specificity, it might be worthwhile to investigate if epigenetic control is important in the regulation of the symbiotic cell-specific genes. The nodule-specific expression of putative retrotransposons, which are usually epigenetically silenced, and the Dicer 1-like ribonuclease III gene, which may have a role in epigenetic regulation, as well as the identification of small RNAs potentially targeting NCR genes  are all in agreement with such an epigenetic control of the symbiotic cell-specific genes.
Plant genomes contain large numbers, several hundreds to thousands, of resistance genes (R) of the NB-LRR family that recognize specific pathogen effectors and trigger resistance. Silencing by microRNAs has been proposed as a mechanism to avoid unregulated expression of R genes which may be a threat to the plant and represent a fitness cost . Why M. truncatula maintains such a large repertoire of NCR genes is not known. It is also not known whether closely related legumes of the IRLC have an arsenal of NCRs of similar size. However, it seems likely to us that expressing such a large gene family might be a fitness cost for the plant that is not to be neglected. Therefore, keeping the whole gene family under a very tight regulatory control might be essential for the plant.
From the transcriptome data mining and experimental confirmation described here, we can conclude that apart from very few exceptions, the hundreds of NCR genes encoding defensin-like peptides are only activated during nodule formation. They are not expressed in other plant organs, during pathogen attack or abiotic stress. In nodules, they are not yet activated during the very early stages before symbiotic nodule cells are formed and rhizobia are released in symbiosomes within the host cells. NCR genes are also not involved in symbiosome and bacteroid degradation during nodule senescence since their gene expression shuts down when senescence is initiated. However, the expression pattern of NCRs in successive waves during nodule formation suggest that the bacteroids are the only targets of the peptides and that subsets of the peptides might be involved in bacteroid differentiation and other subsets in bacteroid functioning. The NCR genes are among the most specifically expressed genes in M. truncatula. Moreover, when activated in nodules, their expression level is among the highest of all genes. Together, these data show that the NCR gene expression is subject to an extreme tight regulation and is only activated during nodule organogenesis in the symbiotic cells.
Analysis of MtGEA data
The MtGEA transcriptome compendium was downloaded from the website of the Samuel Roberts Noble foundation (http://mtgea.noble.org/v3/). The data from [22, 23] were obtained from the NCBI Gene Expression Omnibus (accession n° GSE53406 and GSE43354 respectively). All the data were imported in Excel (Additional file 1: Table S1) for extracting the expression profiles of the 334 NCR probe-sets and for further treatments. The NCR probe-sets on the Affymetrix Medicago GeneChip, which was used for the MtGEA transcriptome compendium, were obtained by BLASTn searches on the MtGEA website (Additional file 1: Table S1). Each individual NCR nucleotide sequence resulted in the identification of multiple probe-sets due to the homology between NCR gene sequences. In total 334 different probe-sets were retrieved. This collection represent likely nearly all NCR probe-sets present on the Affymetrix Medicago GeneChip and the remaining genes identified in Young et al.  and Zhou et al.  are missing from these arrays because they were not yet annotated at the time of array design.
Cluster analysis of the complete MtGEA dataset was performed using the MeV software package (http://sourceforge.net/projects/mev-tm4/). Briefly, the Excel datasheet extracted from MtGEA was analyzed using the Euclidean distance application with average linkage settings. Heat maps were generated with MeV and histograms and graphs with Excel.
Calculations were performed on the MtGEA dataset in Excel. For the normalization of expression levels in N tissues, the relative expression P t/g of a gene g in a tissue t was calculated as P t/g = W t/g /Σ1≤t≤NW t/g where W t/g is the expression level of the gene g in the tissue t. The Shannon entropy E g of gene g is calculated as E g = Σ1≤t≤N-Pt/glog2(Pt/g). E g ranges from zero for genes expressed in a single tissue to log2(N) for genes expressed uniformly in all tissues considered. Heat maps of entropy values were generated by the MeV software package.
Transcriptome analysis of hand-dissected nodule zones
Using the leghemoglobin colour gradient along the nodule as guideline, five regions from 28 dpi nodules were hand-dissected as previously described . These samples correspond to the nodule tissues from the most apical part of the nodule with the youngest symbiotic cells to the most proximal part containing the oldest symbiotic cells. Sample I is the meristem and the underlying few cell layers of post-meristematic cells which start the infection and differentiation process. Sample II corresponds mainly to the infection and differentiation zone II. Sample II-III corresponds essentially to the interzone II-III. Sample III is the nitrogen fixation zone III, easily characterized by its pink color due to the accumulation of high amounts of leghemoglobin and finally sample IV is the senescence zone IV that is recognized by its green color resulting from the accumulation of biliverdin, a product of the catabolism of leghemoglobin-derived heme. It should be noted that each of these hand-dissected samples is enriched for the indicated zone but can contain cell layers form the adjacent zones as well.
Total RNA extraction and purification were conducted as described . For hybridization onto the Affymetrix M. truncatula Genechip Array probes were synthesized and labelled from 500 ng RNA using the Gene Chip 3′IVT express kit following manufacturer’s guidelines (Affymetrix). Global normalization of expression was carried out using the Robust Multiarray Average Express software .
Transgenic plants and GUS analysis
The promoters of NCR001, NCR084 and NCR121 (respectively 2.5 kb, 1.5 kb and 1 kb fragments upstream of the ATG) were obtained by an Amplified Fragment-Length Polymorphism (AFLP) based PCR protocol as described  and recombined in the Gateway vector pDONRP4-P1R according to the manufacturer’s instructions (Invitrogen). Primers used for the amplification and cloning of the promoters were: attB4FWD_NCR001, GGGGACAACTTTGTATAGAAAAGTTGGTTGTCCTTATTAGAGCGCC; attB1REV_NCR001, GGGGACTGCTTTTTTGTACAAACTTGTATGTTTCATCCTTTGAACG; attB4FWD_NCR084, GGGGACAACTTTGTATAGAAAAGTTGGCGAGAAAGGAAGGGAAGAA; attB1REV_NCR084, GGGGACTGCTTTTTTGTACAAACTTGTATTTTTCTCCCTTTACATG; attB4FWD_NCR121, GGGGACAACTTTGTATAGAAAAGTTGTCCTTCTATGCATGTTCAAA; attB1REV_NCR121, GGGGACTGCTTTTTTGTACAAACTTGGTTTTTCCCTCTTTATAGGT.
Entry clones for the GUS ORF and the 35S terminator were obtained in the Gateway vectors pDONR221 and pDONRP2R-P3, respectively . Entry clones were recombined in the binary vector pKm43GW . Leaf explants from the M. truncatula line R108 were transformed using Agrobacterium tumefaciens according to the method described in Cosson et al. .
For GUS analysis, three independent T2 transgenic lines were each time analyzed to avoid positional effects of the transgene insertion. No pattern variations were observed between independent lines. Untransformed plants and the constitutive GUS line pG3.3 (35S promoter fused to GUS)  were used as negative and positive controls respectively. For nodulation kinetics, R108 plants were cultivated on BNM agar plates and inoculated with OD600 = 0.1 suspensions of S. meliloti strain 1021 or Sinorhizobium arboris strain B554 (synonymous strain names LMG 14919 and HAMBI 1552)  which is an excellent symbiont of M. truncatula R108 forming numerous large, nitrogen fixing nodules. Samples were collected at indicated time points and embedded in 6% agarose. Tissue sections of 70 μm were prepared with a Leica VT1200S vibratome. GUS staining was done as described  and was allowed to proceed for 1 h (Additional file 2: Figure S6). Overnight staining did not alter the expression patterns (data not shown). The pattern of expression of the NCR genes in nodules induced by both Sinorhizobium strains were very similar.
For all pathogen assays, plants were cultivated on perlite/sand (3/1 vol/vol) substrate and watered with a commercial nutrient solution. Six weeks old plants were transferred to a growth chamber with saturating humidity the day before the inoculations and remained in these conditions all along the assay. Dickeya dadantii 3937, Pseudomonas syringae pv. tomato DC3000 and its hrcC derivative strain were cultivated at 30°C in LB medium. Inocula of OD600 = 0.1 were resuspended in 10 mM MgCl2 and were syringe infiltrated in the terminal leaflet of 5–8 leaves per plant. Sterile 10 mM MgCl2 solution was infiltrated as mock control. Botrytis cinerea strain B05.10 was cultivated on PDA medium  at 20°C. Spores were collected in ½ potato dextrose broth with 0.01% Tween 20 and inocula were normalized to 106 spores/mL using a Malassez cell. Five microliter drops of mock/inoculum were put on 5 to 8 terminal leaflets per plant. Symptoms were scored at 1, 2 or 7 dpi and leaflets were collected for GUS staining. For wounding experiments, the terminal leaflet of 5–8 leaves per plant were pinched with forceps and collected 24 hours post wounding. Staining for all infections or treatments was allowed for 24 hours in the GUS staining solution at 37°C. The leaflets were transferred to bleach to remove chlorophyll before photographing.
Antibodies and immunolocalization
The mature region of the NCR122 peptide was amplified from cDNA and cloned into the expression vector pBADgIII/A (Invitrogen). Recombinant proteins were purified according to the manufacturer’s instructions and used for immunization of rabbits by a commercial service (Agro-bio). Immunolocalisations were done exactly as described before . For the SYTO13 nucleic acid staining, nodules sections were incubated for 5 minutes with 1 μM SYTO13 in H2O. Immuno- or SYTO13-stained sections were mounted in deionised water for confocal imaging. Fluorescence images were acquired at 1024×1024 pixels resolution with the confocal laser scanning microscope TCS SP2 from Leica, using 10X water-immersion and 63X oil-immersion objectives and Leica software. Images were processed with Adobe Photoshop for adjustment of contrast and brightness.
We thank Eric Giraud and Olivier Pierre for critical reading of the manuscript. Research in the P.M. laboratory is supported by the Agence Nationale de la Recherche, grant ANR-13-BSV7-0013-01. E.K. has been supported by the “SYMBIOTICS” Advanced Grant of the European Research Council Grant 269067.
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