Genome mining for ribosomally synthesized and post-translationally modified peptides (RiPPs) in anaerobic bacteria

Table 5 Detected LAP gene cluster

	Phylum	Locus Tag of dehydrogenase	Similar to^†	Reference^#
Clostridium botulinum A2 BoNT/A2 Kyoto-F	Firmicutes	CLM_0573	clostridiolysin S
Clostridium botulinum A BoNT/A1 ATCC 19397	Firmicutes	CLB_0528	clostridiolysin S
Clostridium botulinum A BoNT/A1 Hall	Firmicutes	CLC_0561	clostridiolysin S
Clostridium botulinum BoNT/B1 Okra	Firmicutes	CLD_0261	clostridiolysin S
Clostridium botulinum BoNT/A3 Loch Maree	Firmicutes	CLK_3698	clostridiolysin S
Clostridium botulinum Ba4 str. 657	Firmicutes	CLJ_B0564	clostridiolysin S
Clostridium botulinum F230613	Firmicutes	CBF_0535	clostridiolysin S
Clostridium botulinum H04402 065	Firmicutes	H04402_00508	clostridiolysin S
Clostridium botulinum A ATCC 3502	Firmicutes	CBO0487	clostridiolysin S	[50]
Clostridiumbotulinum F str. Langeland	Firmicutes	CLI_0567	clostridiolysin S
Thermoanaerobacter mathranii mathranii A3, DSM 11426	Firmicutes	Tmath_0475	**
Brachyspira hyodysenteriae WA1	Spirochaetes	BHWA1_02354
Brachyspira pilosicoli 95/1000	Spirochaetes	BP951000_0919	*
Brachyspira intermedia PWS/A	Spirochaetes	Bint_2324	*
Brachyspira murdochii 56–150, DSM 12563	Spirochaetes	Bmur_0997	*

*All strains share an identical precursor peptide sequence; **no precursor peptide could be annotated; ^†Cluster shows similarities to characterized RiPP cluster; ^#Cluster was previously detected by genome mining approaches.

ISSN: 1471-2164