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BMC Genomics

Open Access

FGF negative regulation during early myogenesis

BMC Genomics201415(Suppl 2):O23

Published: 2 April 2014


Feedback LoopEmbryonic DevelopmentNegative FeedbackReceptor Tyrosine KinaseFine Tuning

Negative regulators of signal transduction cascades play critical roles in controlling different aspects of normal embryonic development. Sprouty2 negatively regulates FGF signaling and Receptor Tyrosine Kinases (RTK) and is important in differentiation, cell migration and proliferation. In vertebrate embryos, Sprouty2 is expressed in pre-segmented mesoderm and in forming somites. Expression is maintained in the myotome until late stages of somite differentiation. However, its role and mode of action during somite myogenesis is still unclear. In the current study, we analysed chick Sprouty2 expression and showed that it overlaps with that of Myogenic Regulatory Factors (MRF) MyoD and Mgn. Targeted mis-expression of Sprouty2 led to inhibition of myogenesis, whilst its C-terminal domain interference led to an increased number of myogenic cells by stimulating cell proliferation. Our results show that Sprouty2 plays crucial role in regulating chick myogenesis by fine tuning of FGF signaling through a negative feedback loop.

Authors’ Affiliations

Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia
Faculty of Science, Zoology Department, Minia University, El-Minia, Egypt
School of Biological Sciences, Department of Cell and Developmental Biology, University of East Anglia, Norwich, UK


© Abu-Elmagd and Münsterberg; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.