- Research article
- Open Access
Molecular cloning and sequence analysis of hamster CENP-A cDNA
© Figueroa et al; licensee BioMed Central Ltd. 2002
- Received: 27 February 2002
- Accepted: 3 May 2002
- Published: 3 May 2002
The centromere is a specialized locus that mediates chromosome movement during mitosis and meiosis. This chromosomal domain comprises a uniquely packaged form of heterochromatin that acts as a nucleus for the assembly of the kinetochore a trilaminar proteinaceous structure on the surface of each chromatid at the primary constriction. Kinetochores mediate interactions with the spindle fibers of the mitotic apparatus. Centromere protein A (CENP-A) is a histone H3-like protein specifically located to the inner plate of kinetochore at active centromeres. CENP-A works as a component of specialized nucleosomes at centromeres bound to arrays of repeat satellite DNA.
We have cloned the hamster homologue of human and mouse CENP-A. The cDNA isolated was found to contain an open reading frame encoding a polypeptide consisting of 129 amino acid residues with a C-terminal histone fold domain highly homologous to those of CENP-A and H3 sequences previously released. However, significant sequence divergence was found at the N-terminal region of hamster CENP-A that is five and eleven residues shorter than those of mouse and human respectively. Further, a human serine 7 residue, a target site for Aurora B kinase phosphorylation involved in the mechanism of cytokinesis, was not found in the hamster protein. A human autoepitope at the N-terminal region of CENP-A described in autoinmune diseases is not conserved in the hamster protein.
We have cloned the hamster cDNA for the centromeric protein CENP-A. Significant differences on protein sequence were found at the N-terminal tail of hamster CENP-A in comparison with that of human and mouse. Our results show a high degree of evolutionary divergence of kinetochore CENP-A proteins in mammals. This is related to the high diverse nucleotide repeat sequences found at the centromere DNA among species and support a current centromere model for kinetochore function and structural plasticity.
- Satellite Repeat
- Eleven Amino Acid
- 1623nt cDNA
- Centromere Drive
- Centromeric Repeat Sequence
Centromeres provide the essential functions for chromosome segregation. They act as regulators of mitosis and meiosis by controlling attachment of chromosomes to the spindle and regulating progression into anaphase [1–4]. In mammalian cells, the centromere is associated with large blocks of heterochromatin comprised of highly repetitive satellite DNA of unconserved sequence repeats among species. It have been hypothesized that centromere function is therefore not specified directly by DNA sequence, but rather by higher order DNA or chromatin structure . Centromeric DNA and protein components have been suggested to evolve rapidly in evolution and be responsible for the organization of functional centromere of emerging species .
CENP-A is a 17 kD histone H3 variant with homologous polypeptides being described from human to yeast [7–9]. Different approaches have indicated that CENP-A is required for the assembly of kinetochore and activation of the centromere [10–12]. The human protein shows more than 60% sequence identity to H3 at the C-terminal histone fold domain. The highly divergent N-termini of CENP-A is localized outside the nucleosome and should serve to interact with other kinetochore proteins including histones [4, 13]. This unique N-terminal charged region forms a major epitope in the induction of specific autoantibodies against centromere in autoimmune patients [14, 15]. The overall homology between homologous CENP-A proteins and the evolutionarily unconserved satellite repeat DNAs at the kinetochore described for many species, constitute a major unresolved enigma in modern biology for understanding the structural organization and function of the centromere.
As suggested in a recent report on centromere DNA sequences divergences found in eukaryotes, those changes observed in the N-terminal region of several CENP-A proteins, could be explained for their specific recruitment at nucleosomes containing different DNA satellite repeat [6, 17]. The centromere drive model proposed by Malik et al.  imply changes during evolution on centromeric repeat sequences associated with those found on centromeric constitutive antigens such as CENP-A. This specific event could play a role at the distinctive higher-order of chromatin structure at mammalian centromeres . Thus, understanding the relationships between CENP-A primary structure and DNA repeat organization at the centromeres may provide a clue into the structural requirements needed for assembly of a kinetochore.
Hamster CENP-A and its human and murine homologous proteins are highly conserved at the C-terminal domain. However they have significant amino acid differences at the N-terminal tail, including a serine residue at position 7 which is missing in hamster and mouse CENP-A, but it is phosphorylated by Aurora B kinase in humans for a role in cytokinesis. Further, the N-terminal domain of hamster CENP-A is five and eleven amino acid shorter than those homologous proteins of mouse and human respectively. Thus, the divergences found in the N-terminal tail of CENP-A in different species during evolution, according to a current centromere model could be correlated with those variations widely described on centromeric DNA repeat sequences [6, 17].
Isolation of a hamster CENP-A cDNA
A CHO cDNA expression library constructed in lambda ZAP (Stratagene) was screened with a human CENP-A cDNA probe resulting in the isolation of several positive clones. One of these resulted to be a hamster full length CENP-A clone described here. cDNA was subcloned in pBluescript vector (Stratagene) and DNA sequence analysis was performed in both strands by the dideoxy method using T7 DNA polymerase (Amersham Biosciences) and 5'-[α-35S]-labeled dATP (NEN Life Science Prod. Zaventem, Belgium). DNA sequences were analysed in EMBL databases using BLAST and Clustaw softwares.
We thanks Dr. Kevin Sullivan of Scripps Research Institute, La Jolla CA, for supply us with a human CENP-A cDNA probe. This work was supported in part by grants from Comisión Interministerial de Ciencia y Tecnología (PB97-1354) and by Plan Andaluz de Investigación (CVI 167) of Junta de Andalucía. We are grateful to Unidad de Radioisótopos of University of Cádiz for their assistance in using their facilities.
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