Table 3 Quantitative RT-PCR primers and probes

(-)-GGCATCCAAATTCTCCAAGAAA

FAM-TGCTGCCTCTCCTGAGTAGGGTGGGT-TAMRA

CDK5R1

(+)-TCCTACATGGGCAACGAGATC

Y = -3.315x + 45.983 R2 = 0.99

(-)-CCAAAAGGCCTCCTTGCA

FAM -TACCCGCTCAAGCCCTTCCTGGTG -TAMRA

COL8A1

(+)-CCGAGCTAACCGCACCTTT

Y = -3.143 x +43.294 R2 = 0.99

(-)-GTCTGCGGGTTGTAGTTCTGTCT

FAM -AGTGAAGTTTAACAAACTGCTGTATAACGG -TAMRA

FZD

(+)-AGCCTCAAAGGTTCCACATCTC

Y = -3.515 x + 47.092 R2 = 0.99

(-)-AGGTCACTTCCAGTGTAACACAAATT

FAM -TGAGAAAAGAGCAGGGAGGTGGTTGTCA -TAMRA

GPLD1-1

(+)-CAGATTGAAGATTTCACTGCATTTC

Y = -3.653 x + 49.086 R2 = 0.99

(-)-CATCAAAATGCTCACCATGGA

FAM -TCTGCCCACCTCTCTCATGCTGAATCAC -TAMRA

GPLD1-2

(+)-CTTTTGCCTGTAGTAGTAAATTGCTTTTA

Y = -3.168x + 46.599 R2 = 0.99

(-)-AACTGGCCATATAAC CAAAGGTGTT

FAM -TGAATGGTGTTTATTAAACCCTTATGGTCGATATTTCC-TAMRA

HIRIP5

(+)-GCTGCCCTAGTTCAATCATTACTCT

Y = -3.160x + 43.313 R2 = 0.99

(-)-CGCCTTCTACCTCCGGAATAT

FAM – AAAAATGGAATTCAGAACATGCTGCA-TAMRA

HIST1HIA

(+)-AGGCGTCCTCCGTGGAA

Y = -3.143x + 43.294 R2 = 0.99

(-)-ATGCACCCGTTGCCTTAGTT

FAM – AGCCCGGCGCCTCAAAGGTG-TAMRA

NEDD8

(+)-TGACCGGAAAGGAGATTGAGA

Y = -3.458x + 48.686 R2 = 0.99

(-)-CCACACGCTCCTTGATTCG

FAM – TGACATTGAACCTACAGACAAGGTGGA-TAMRA

PTH

(+)-GCATAACCTGGGAAAACATCTGA

Y = -3.063x + 45.215 R2 = 0.99

(-)-TGCACATCCTGCAGCTTCTT

FAM -TCGATGGAGAGAGTAGAATGGCTGC-TAMRA

RAB31

(+)-CCCCTGAAGGATGCTAAGGAA

Y = -3.303x + 48.517 R2 = 0.99

(-)-AGCATTTTTTGCACTTGTCTCAAC

FAM – ACGCTGAATCCATAGGTGCCATC-TAMRA

SNRPD3

(+)-GGCACAGCTGGAGCAGGTAT

Y = -3.177x + 47.200 R2 = 0.99

(-)-CTTCAGCATGTCAGGCAAAATC

FAM – ATCCGTGGCAGCAAAATCCGC -TAMRA

β-actin

(+)-GGCACCCAGCACAATGAAG

Y = -3.127x + 44.733 R2 = 0.99

(-)-GCCGATCCACACGGAGTACT

FAM-TCAAGATCATTGCTCCTCCTGAGCGC-TAMRA

gp100

(+)GGTTCCTTTTCCGTCACCCT

Y = -3.282x + 45.987 R2 = 0.99

(-)CTCACCGGACGGCACAG

FAM-ACATTGTCCAGGGTATTGAAAGTGCCGAGAT-TAMRA