Figure 2

Motifs in 5'-flanking region of cIRK1 DNA sequence of the 5'-flanking region (from -417 to +33) is shown. Highlighted are the positions of two E boxes (asterisks), a NF-κB site (heavy underline), a putative TATA box (plus signs), four Sp1 sites (dashed double underlines), and an NF-Y binding site (dotted underline). Transcription initiation sites are shown in bold, with the most downstream site numbered +1. The 5'-ends of the fragments used in the promoter assays are shown with slashes and the construct names (see also Figure 4).