- Research article
- Open Access
Duplication and relocation of the functional DPY19L2 gene within low copy repeats
© Carson et al; licensee BioMed Central Ltd. 2006
- Received: 13 January 2006
- Accepted: 09 March 2006
- Published: 09 March 2006
Low copy repeats (LCRs) are thought to play an important role in recent gene evolution, especially when they facilitate gene duplications. Duplicate genes are fundamental to adaptive evolution, providing substrates for the development of new or shared gene functions. Moreover, silencing of duplicate genes can have an indirect effect on adaptive evolution by causing genomic relocation of functional genes. These changes are theorized to have been a major factor in speciation.
Here we present a novel example showing functional gene relocation within a LCR. We characterize the genomic structure and gene content of eight related LCRs on human Chromosomes 7 and 12. Two members of a novel transmembrane gene family, DPY19L, were identified in these regions, along with six transcribed pseudogenes. One of these genes, DPY19L2, is found on Chromosome 12 and is not syntenic with its mouse orthologue. Instead, the human locus syntenic to mouse Dpy19l2 contains a pseudogene, DPY19L2P1. This indicates that the ancestral copy of this gene has been silenced, while the descendant copy has remained active. Thus, the functional copy of this gene has been relocated to a new genomic locus. We then describe the expansion and evolution of the DPY19L gene family from a single gene found in invertebrate animals. Ancient duplications have led to multiple homologues in different lineages, with three in fish, frogs and birds and four in mammals.
Our results show that the DPY19L family has expanded throughout the vertebrate lineage and has undergone recent primate-specific evolution within LCRs.
- Functional Gene
- Segmental Duplication
- Recent Gene Duplication
- Neutral Selection
- Ancient Duplication
Gene duplication is an important factor in evolution. These duplications can arise during the expansion of low copy repeats (LCRs), or segmental duplications, which make up a significant proportion of the human genome sequence [1, 2]. The high sequence identity (>90% over >5 kb) shared between these DNA segments indicates that they have arisen over the past 35 million years (MY) of primate evolution and in some instances are human specific. Detailed analyses of LCRs in humans have shown that these repeats are often interspersed, rather than tandemly duplicated [1, 3]. LCRs are observed both interchromosomally, with enrichment in pericentromeric and subtelomeric chromosomal regions , and intrachromosomally, with enrichment in euchromatic sequences.
LCRs have important consequences in vertebrate gene evolution. Traditionally, the major force in genome evolution was thought to be nucleotide substitutions. However, gene duplications have been shown to significantly contribute to evolution by acting as molecular substrates for divergence [5, 6]. In fact, the estimated rate of duplicate gene fixation (1/gene/100 MY) [7–9] is similar to or greater than the estimated nucleotide substitution rate at silent sites (0.1–0.5/site/100 MY) . Duplicate genes arise by several different mechanisms: unequal crossing over and retroposition, which give rise to small duplications, and chromosomal duplication and whole genome polyploidization, which involve much larger amounts of DNA . Considering that the last whole genome polyploidization event in the land vertebrate lineage is estimated to have occurred more than 600 MY ago , duplications of this nature are not expected to play a role in primate speciation. Instead, smaller duplications, such as those creating LCRs, which maintain gene structure and organization, are predicted to have significantly affected recent primate evolution. This theory is supported by the finding that segmental duplications make up a significant proportion of primate genomes, with estimates of around 5% in the human genome [1, 2, 4]. Although the LCR composition of mammalian genomes has been recently examined, an in-depth investigation of the gene content in LCRs and their role in gene evolution has not been undertaken.
Studies have shown an unexpectedly large complement of functional gene duplicates in most genomes [13, 14], which can be accounted for by two of the fates for duplicated genes. One of these fates, subfunctionalization, occurs when both copies of a gene degenerate in a complementary fashion, such that both copies are required to perform the ancestral functions [15, 16]. The other fate is neofunctionalization, in which positive selection for beneficial mutations occurs, allowing one of the gene copies to diverge rapidly and adopt a new function . Subfunctionalization is predicted to develop more frequently than neofunctionalization because it exploits common degenerative mutations rather than relying on rare beneficial mutations.
The third and most common fate for copies of a gene following duplication and fixation is for one of the copies to become nonfunctional through the accumulation of deleterious mutations. These mutations are tolerated when duplicate genes are functionally redundant, relaxing the selection on at least one of the two copies. In humans, it is estimated that duplicate genes have an average half life of 7.5 MY . After a copy is silenced, a nonfunctional remnant will exist until it is deleted or mutated beyond recognition. These remnants are known as pseudogenes. Pseudogenes can also be created by partial duplications that exclude key sequences or regulatory regions of genes. Several recent studies have evaluated the pseudogene content in the human genome [18, 19] and have found that their number may equal or even surpass the number of functional genes.
Although pseudogenization restores the proteome to its pre-duplication state, it may passively affect adaptive evolution. Since selection is often relaxed on both copies of the gene after duplication, either the ancestral or the progeny copy can be inactivated. Pseudogenization of the ancestral copy constitutes gene relocation. Much like larger rearrangements, relocation of unique genes to different chromosomes can contribute to reproductive isolation and speciation .
Physical organization of the low copy repeats
To define the duplication boundaries and determine their structures, two programs, Blast 2 sequences  and mVISTA [26–28] were utilized. For these analyses, the sequences of the LCRs on Chromosome 7 were obtained from the Chromosome 7 annotation project sequence (CRA_TCAGchr7.v2) while the sequence on Chromosome 12 was acquired from NCBI's public Build 35 (see Additional file 1). Using the two programs we were able to determine the size and sequence content of the LCRs (see Additional file 2). We were also able to resolve the complex modular structure of the duplications, tracing much of the duplicated DNA back to LCR7A by a best match analysis (Fig. 2B). Overall, the eight LCRs comprise more than 1 Mb of duplicated DNA with sequence identities ranging from 94–98% (see Additional file 2).
Identification of transcripts in low copy repeats
The first transcript from LCR7A [Genbank:AB020684] has 22 exons and a complete open reading frame (ORF) coding for 675 amino acids. We defined an ORF as complete when it codes for >100 amino acids and is uninterrupted between a start codon in the first exon and an inframe stop codon in the last exon. This is a conservative definition of a complete ORF and may exclude transcripts with functions that do not require the complete CDS, such as those undergoing neo- or subfunctionalization. Transcripts with complete ORFs were classified as genes. Although the function of AB020684 is unknown, in silico analysis indicated that it encodes a protein with homology to the C. elegans protein DPY-19 (see Additional files 3 and 4). Therefore, we called this gene DPY19L1 (Fig. 3A). Ten exons from this gene (exons 4 to 13) appear to be duplicated in a known transcript within LCR7B [Genbank:AK026768]. Along with these ten exons, AK026768 has an additional two upstream and five downstream exons that fall outside the duplicated sequence, giving it a total of 17 exons. However, unlike DPY19L1, this transcript does not appear to encode a complete ORF because it has STOP codons in exons 2 and 11. This transcript, which we named DPY19L1P1 (Fig. 3A), was classified as a pseudogene. We defined a pseudogene as a duplicated transcript that has lost its original function due to incomplete duplication or at least one interruption of the ORF.
The second transcript from LCR7A [Genbank:BC066987] also has 22 exons. This transcript does not have a complete ORF because it has STOP codons in exons 6 and 7. Duplications of all 22 exons of this transcript were seen in two other regions: LCR7C and LCR7D. The transcript in LCR7C [Genbank:AL833344] is a gene because it has a complete ORF coding for 758 amino acids. In silico analysis of this ORF again showed homology to the C. elegan s gene DPY-19, although with a lower percent identity (38% amino acid identity, compared with 43% identity for DPY19L1) (see Additional files 3 and 4). Therefore, we named this gene DPY19L2 (Fig. 3B). Like the second transcript on LCR7A, the transcript on LCR7D [Genbank:AL834175] has inframe STOP codons, caused by insertions of LTR repeats in exon 3. These inactivated transcripts on LCR7A and LCR7D are classified as pseudogenes, and we named them DPY19L2P1 and DPY19L2P2 respectively (Fig. 3B).
The two genes and three pseudogenes make up a set of duplicated known transcripts within the eight LCRs. To identify novel transcripts that belong within this set, we used the two coding genes as queries for UCSC BLAT searches  against the human genome Build 35 sequence. Using sequence identity to uncharacterized mRNA and EST transcripts, we were able to identify three additional pseudogenes that arose from partial duplications. One transcript in LCR7B, DPY19L1P2, shows high identity with DPY19L1, while two other transcripts, DPY19L2P3 in LCR7E and DPY19L2P4 in LCR7F, share high identity with DPY19L2 (Fig. 3A, 3B). The relationships between these transcripts are illustrated by an analysis of their phylogeny (see Additional file 5).
Genomic relocation of DPY19L2
Although both DPY19L1 and DPY19L2 share homology to the C. elegan s gene DPY-19 and are within a set of related LCRs, these two genes are not recent duplicates of each other. We reached this conclusion because the region of LCR7A that contains DPY19L1 does not show high sequence identity with the region of LCR7C that contains DPY19L2 (Fig. 2B). Instead, we attribute their similarity to an ancient duplication that occurred prior to mammalian divergence. This is supported by two observations: the percent nucleotide identity between the two transcripts is ~76% (less than the 90% cutoff typically used for recent duplications [1, 2]; see Additional file 3) and both genes are also present in mouse. The two mouse genes, Dpy19l1 and Dpy19l2, share ~72% nucleotide sequence identity with each other and are ~54.3 kb apart on mouse Chromosome 9 in a region syntenic to LCR7A on human Chromosome 7p14.3 (Fig. 1). Importantly, Blastn analysis indicates that these two genes, and their genomic regions, are unique in the mouse genome and do not appear to have been recently duplicated within LCRs. Notably, the functional Dpy19l2 is syntenic to the human pseudogene DPY19L2P1, not to the functional DPY19L2 on Chromosome 12. This indicates that the ancestral copy of DPY19L2 was present on Chromosome 7 (Fig. 1). This is confirmed by our analysis of the genes in dog and rat. Segmental duplication of this region onto Chromosome 12q14.2 established two copies of the functional gene. Initial functional redundancy relaxed the selection on one or both of the copies of this gene, allowing the fixation of an inactivating pseudogenization event. In this case, the ancestral copy on 7p14.3 was pseudogenized while the copy on 12q14.2 remained functional. Thus, DPY19L2 has undergone gene relocation within a recent LCR (Fig. 1).
Neutral evolution of recently duplicated transcripts
We analyzed the three known pseudogenes within the LCRs, each of which contains a substantial duplication of the original ORF. We hypothesized that if these transcripts are no longer functional, their sequences would not be under selectional constraint. Hence, they would be evolving neutrally. We looked for evidence of selection by two methods, codon position bias of nucleotide changes and Ka/Ks; each assumes nucleotide changes accumulate evenly in the three codon positions during neutral evolution. As it is possible that truncation following duplication can result in a new gene, with purifying selection 5' and neutral evolution 3' of the new STOP codon, we selected the largest ORF in each of the pseudogenes for this analysis. In each case, the largest ORF observed corresponded to a truncation of the original ORF, rather than a frameshift.
Nucleotide change analysis showing neutral evolution of pseudogenes
Nucleotide p-distance at Codon Position
Pseudogene or Mouse gene
Distributions of nucleotide changes affect the synonymous (Ks) and non-synonymous (Ka) substitution rates. In purifying selection, we expect the synonymous rate to be much higher than the non-synonymous rates (Ka/Ks<1), whereas in neutral selection the rates are expected to be very similar. Much like the first analysis, we see that the substitution rate between the functional human and mouse genes suggests purifying selection, while the Ka/Ks values within the inactive genes' ORFs are much higher (Table 1). Most likely, this increase in Ka/Ks indicates relaxed or neutral selection of the pseudogenes.
DPY19-like gene family
Along with DPY19L1 and DPY19L2, homology searches identified two other human genes that have amino acid identity to the C. elegans gene DPY-19 (see Additional files 3 and 4). These two genes, DPY19L3 and DPY19L4, are located on Chromosomes 19q13.11 and 8q22.1, respectively, and do not reside in any segmental duplications. Both of these transcripts are known genes spanning 18 exons and are covered by full-length mRNA transcripts ([Genbank:AK126757], encoding 716 amino acids, and [Genbank:AK123682], encoding 723 amino acids, respectively). Similar to DPY19L1 and DPY19L2, the functions of these genes are uncharacterized.
We used NCBI's BLAST  and UCSC's BLAT  tools to identify proteins homologous to DPY-19 in other animals (see Methods). These protein sequences were then aligned using ClustalW . With the multiple alignment file, a neighbour-joining phylogenetic tree was constructed using MEGA3  (Fig. 4B). As a test of inferred phylogeny, bootstrap values were calculated using 250 replications. From this analysis we can see that invertebrate animals such as nematodes (C. elegans) and deuterostomes (C. intestinalis) have a single DPY-19 gene, while all vertebrates examined have at least three homologous copies. This suggests that multiple duplications have occurred during vertebrate evolution. The first duplications appear to have occurred prior to the divergence of the fish lineage, giving rise to two additional copies, DPY19L3 and DPY19L4, which are present in all vertebrates. An additional duplication, generating DPY19L2, appears to have arisen prior to mammalian divergence. This gene appears in all mammals examined, including the metatherian opossum (M. domestica). However, it is unclear whether this duplication occurred before or after the divergence of chicken. Although chicken is lacking DPY19L2, one of its proteins is placed in the DPY19L1 clade (with a relatively high bootstrap value of 77%) rather than grouping with the DPY-19 proteins. This could indicate that the duplication leading to DPY19L2 has occurred before chicken divergence with subsequent gene loss in chicken (or the second copy may not be represented in the genome sequences available for the chicken due to the incomplete nature of its genome assembly). Alternatively, the duplication could have occurred after chicken divergence, with the chicken protein maintaining more similarity to DPY19L1 due to an accelerated evolution of DPY19L2 relative to DPY19L1 (longer branch lengths of DPY19L2 proteins are seen in Fig. 4B). Through these observations, we estimate that the duplication that generated DPY19L2 arose between 173 (Marsupilia) and 360 (Lissamphibia) MYA (based on divergence times in ). Additional trees were built using different phylogenetic methods (including minimum evolution and maximum parsimony) to confirm the observed phylogeny. Overall, these trees had a highly similar topology to the tree presented in Fig. 4B (data not shown).
Structure and conservation of DPY-19 homologs
DPY-19 is a novel transmembrane protein that is required for the proper polarization and migration of neuroblasts in C. elegans. It has been shown to express weakly in the QL and QR neuroblasts, their neighbouring epidermal cells, and in dorsal and ventral body muscle cells . To ensure that the four human homologues have similar structures to this gene, we compared their domain architectures using SMART  and Pfam . DPY-19 does not have any predicted SMART or Pfam domains, but does have 10 predicted transmembrane domains, consistent with its characterization as a transmembrane protein. Similarly, the four human homologues have 9 to 11 predicted transmembrane domains, which suggest that they share a similar protein structure with DPY-19.
Using the multiple species amino acid alignment, we were able to identify putative motifs with high conservation using the program MEME . Three putative motifs, each at least 50 amino acids in width and present in at least 40 of the 42 proteins, were identified by this program. All three putative domains are significantly more conserved at the amino acid level than the rest of the proteins (P-value < 0.0001; See Methods). However, two of these motifs contained one or more transmembrane domains in a subset of the proteins. Therefore, the conservation in these two regions is most likely due to the presence of a transmembrane domain. The third motif, which was the largest and most significant motif identified by MEME, does not match any known domains. This motif is present near the C-termini of the proteins and may represent a novel functionally relevant sequence (see Additional file 6).
Low copy repeats in extant genomes represent the most recent duplications of large stretches of DNA. In humans, sequence and cytogenetic data indicate that the commonly used thresholds are detecting LCRs that have arisen within the past 35 MY of primate evolution [1, 2, 37]. These active, and not uncommon, restructurings within genomes have important implications in molecular evolution. Genes duplicated within LCRs can diverge through substitutions or exon shuffling [38, 39], allowing, in rare circumstances, novel functions to develop. Also, because of their high nucleotide sequence identity, copies of LCRs can act as substrates for large rearrangements, such as deletions, inversions and duplications (reviewed in ). Such large rearrangements are often found to cause genomic disorders, while less severe changes are theorized to be significant factors in genome evolution and speciation.
In this paper, we demonstrate that LCRs can also have a more subtle effect on genome evolution. We first characterize a set of LCRs on human Chromosomes 7 and 12. These LCRs have a complex modular structure with sequence identities ranging from 94–98% (Figure 2B; see Additional file 2). The exact order of the LCR expansion is difficult to ascertain due to this complexity, as well as the similarity in sequence identity between the regions. Even the branching order of the transcripts contained within the LCRs is not easily resolved due to their high sequence similarity (see Additional file 5). After the characterization of these regions and their transcripts, we then describe a novel example of functional gene relocation through duplication and subsequent pseudogenization within a LCR (Fig. 1). This is a type of small chromosomal rearrangement that has been implicated in evolution, and has been suggested to be involved in speciation events through population isolation followed by divergent resolution of duplicate genes [20, 41]. Gene silencing has also been shown to relocate functional genes following whole genome duplication  or subtelomeric duplication  events. The example we report is the first characterization of functional gene relocation within a euchromatic LCR. We propose that, like rearrangements of DNA segments and gene structures, rearrangements in gene order during LCR creation and expansion are a noteworthy mechanism for genome evolution and divergence.
Unlike whole genome duplication, gene relocation following duplication within a LCR can modify a gene's genomic context. Specifically, cis-acting elements neighbouring the ancestral locus may be different from the cis-acting elements neighbouring the descendent locus, causing the positional effects on the gene to be altered. Thus, changes in expression and function are considered to be another consequence of gene relocation. Interestingly, the slight variation in DPY19L2 expression (Fig. 4A) could indicate a change in regulation. One interpretation of this finding is that the change in expression is caused by a difference in cis-acting elements between the functional gene's ancestral and descendant locations.
Our data is consistent with the concept that there are two major fates of gene duplication: retention of both copies or pseudogenization of one of the transcripts. Within the set of LCRs, we were able to identify two homologous functional genes, apparently created by a duplication event of a common ancestor homologous to the C. elegans DPY-19 gene. These genes illustrate how both copies of a gene duplicate can be retained. Preservation of both copies may be due to some selective advantage, either through increased dosage, neofunctionalization, or complementary degeneration leading to subfunctionalization.
These functional genes have been duplicated within LCRs, creating new transcripts. However, for both genes only a single transcript has remained active. The others, although transcribed, are all pseudogenes due to loss of coding potential through partial duplication or accumulation of deleterious mutations (Fig. 3A, 3B). This supports the notion that pseudogenization is the most common fate of gene duplication . In addition, we tested for selection acting on the three duplicated pseudogenes containing a substantial ORF. In two of the three transcripts, selection was relaxed such that they appeared to be undergoing neutral selection. In the third transcript, the selection was relaxed relative to the functional gene, but some purifying selection, indicated by Ka/Ks <1, was still apparent (Table 1). It is important to note that an increased ratio may also represent a mixture of positive and negative selection. However, in combination these analyses show that the inactive transcripts appear to be undergoing neutral evolution, indicating that they have indeed lost their coding potential and are not being selectively maintained. This fits with the perception that most pseudogenes, like non-coding regions, are free of selection [42, 43]. Evidence of selection in one transcript could indicate that it has some actively maintained function. However, the significantly relaxed selection in this case may suggest a recent inactivation that has yet to accumulate enough mutations for neutral selection to be distinguished. In any case, we expect the non-functional remnants of gene duplication to continue to diverge from the original copy, along with the other non-coding regions of the LCR, until eventually all evidence of duplication is erased.
The two functional genes, along with two additional homologues outside of the LCRs, constitute a novel gene family that we designate as the DPY19L family. Although the function of this family is unknown, each member has a large complement of predicted transmembrane domains, indicating that they are functional within one of the cellular membranes. Along with these domains, this family contains a novel putative motif of high conservation (see Additional file 6). The high sequence conservation in this putative motif suggests that its sequence may represent a novel functionally important domain that has been maintained throughout the evolution of this gene family. Interestingly, DPY19L family members have undergone multiple duplications throughout their evolution. Ancient duplications before the divergence of fish led to the creation of two paralogues (DPY19L3 and DPY19L4), while an additional duplication after the divergence of amphibia, yet before the divergence of mammals, created another gene (DPY19L2) (Fig. 4B). Analyses of the human genome sequence indicate that the duplication of these genes is an ongoing process. In fact, all four human paralogues show evidence for relatively recent gene duplication. DPY19L1 and DPY19L2 are both contained in recent low copy repeats, while DPY19L3 and DPY19L4 appear to have processed pseudogenes with duplications of at least 3 exons. Duplications of genes are known to play important roles in gene evolution. The recent duplications of all four human genes suggest that the DPY19L gene family is still evolving and may be an important family in the divergence of species.
The catalogue of LCRs provides an excellent resource for the study of genomic evolution. By characterizing the relationship between sets of LCRs and the genes they contain, we can attempt to understand the mechanisms behind these rearrangements and how they affect evolution. LCRs appear to influence genome composition by affecting genomic sequence structure, gene content and gene order. The last of these influences has not been vigorously investigated. Although this is one of the first observations showing a change in gene location with a set of LCRs, we expect that many other examples are present within LCR-rich genomes, and their identification could lead to a better understanding of genome divergence and speciation.
In silico identification of related LCRs
We queried masked genomic sequence against NCBI's nonredundant (nr) and high-throughput genomic sequence (htgs) databases using Blastn  to identify related copies of the LCRs. Eight regions >5 kb with ≥90% sequence identity were detected. Complete sequences for each of the LCRs was then obtained; for LCRs on Chromosome 7 we used sequence from the Chromosome 7 annotation project (CRA_TCAGchr7.v2), while the LCR sequence on Chromosome 12 was acquired from NCBI's public Build 35. Duplication boundaries were then determined by comparing the regions using Blast 2 sequence  and mVISTA [26–28]. Using the Blast 2 sequence results, we used a C++ program (CircleGraph, H. Wagenaar, unpubl.) to visually display the relationships between each of the LCRs (Fig. 2b). For this analysis, modules, or discrete regions of high identity, were concatenated when they were <5 kb apart or separated by repetitive elements, as long as the modules maintained their orientations between copies. Also, modules <5 kb were eliminated from the analysis, causing some boundaries (LCR7F in Fig. 2B) to appear uncovered by duplications.
Transcripts within the LCRs were identified using full-length mRNAs and spliced ESTs annotated by UCSC's Genome Browser . RT-PCR was used to confirm the expression of transcripts annotated as 'Known Genes'. For novel transcripts, uncharacterized mRNA and ESTs, along with exons predicted by homology analyses, were amplified by RT-PCR and sequenced to determine the complete sequence. Rapid amplification of cDNA ends (RACE) was employed in cases where the 5' and/or 3' gene sequence was uncharacterized. RACE experiments were conducted using marathon-ready cDNA (Clontech) according to manufacturer's instructions. Primer sequences used for these experiments are available upon request.
Genomic organization of each transcript was determined by probing the sequence against the human genome using UCSC's BLAT tool (Fig. 3). Open reading frames (ORF) and protein sequences were determined through conceptual 6-frame translation using NCBI ORF finder . Transcripts having an ORF coding for >100 amino acids, and spanning the entire transcript, from the first to last exon, were annotated as genes. Transcripts not meeting these criteria were classified as pseudogenes.
DNA sequences of the pseudogenes were manually aligned against the ORFs of the two functional genes. These transcripts were truncated such that exons that showed no homology to the functional genes were removed from the alignment. DPY19L3 was also aligned with these sequences and was used as an outgroup in the phylogenetic analysis. This alignment was converted to nexus format and analyzed using PAUP software . A maximum likelihood tree was constructed using the general time reversible plus gamma (GTR+G) model of evolution and bootstrapped with 100 replicates (parameters for nucleotide composition, substitution rates and gamma shape were estimated by PAUP) (see Additional file 5).
Expression analyses of the four human DPY19L genes were performed using RT-PCR in 14 tissues. Primer sequences and conditions are available upon request. For DPY19L2, each of the products was purified using microCLEAN (Microzone) and sequenced to determine contamination of the DPY19L2 expression with pseudogene amplification. Pseudogene amplification was qualified by assessing the presence or absence of transcript specific paralagous sequence variants.
Homology and evolutionary analyses
Using the BLAST  and BLAT  tools, we were able to identify human paralogues of the two protein coding genes detected in our set of LCRs. In addition, we used these same programs to search for orthologues of each of the human genes in a wide range of organisms. Orthologues were identified using BLAT against genome assemblies on the UCSC Genome Bioinformatics webpage . Where no known orthologue is annotated, we assembled transcripts using mRNAs, ESTs and gene predictions. When no transcript information was present, genomic sequences were used to identify conserved exons, taking into account intron/exon boundaries. The trace archive database (NCBI) was searched when gaps in genome assemblies were encountered. ClustalW  was used to align the assembled protein homologues and MEGA3  was used to construct a neighbour-joining phylogenetic tree. The neighbour-joining tree was created using the Jones-Taylor-Thornton matrix  for amino acid evolution and bootstrap values were calculated with 250 replications. Additional trees were built, using alternate phylogenetic methods (neighbour-joining trees under various models of evolution, minimum evolution trees and maximum parsimony trees) to verify the observed phylogeny.
We also used MEGA3 to determine the evolution of the recently duplicated transcripts. We first aligned the coding nucleotides of a gene against the longest homologous ORF in its pseudogene(s) using ClustalW. For this analysis we only used 'Known' transcripts. In the novel transcripts, the ORFs were too short to show significant results. To determine the codon position bias we used MEGA3 to calculate the nucleotide p-distance between the transcripts at each of the coding positions. Next, we used the Nei-Gojobori  p-distance method to calculate the frequency of synonymous substitutions (Ks), the frequency of non-synonymous substitutions (Ka) and the ratio between them (Ka/Ks).
Protein structure analyses
Protein domain architectures, including transmembrane domains, were determined using SMART  and Pfam . Putative functionally relevant motifs were identified using the program MEME . Statistical analysis was performed on these sequences to determine if the predicted motifs were more highly conserved between species. We used MEGA3 to calculate amino acid and nucleotide p-distances inside and outside these motifs, while testing for significant differences with a Wilcoxon signed rank test.
We thank Gianluca Occhi for technical assistance, Dr. Lars Feuk for helpful discussions, Hendrik Wagenaar for the Circlegraph C++ program, and The Centre for Applied Genomics for support. This work was supported in parts by Genome Canada/Ontario Genomics Institute (grant to S.W.S.) and by a Natural Science and Engineering Research Council scholarship (to A.R.C.). S.W.S. is an Investigator of the Canadian Institute of Health Research and International Scholar of the Howard Hughes Medical Institute.
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