Informatic analysis of UniformMu FSTs validated by PCR. (A) Distribution of Mu insertion sites relative to transcribed maize sequences. (B) Schematics of the locus-specific primer design as constrained by available sequences. Left and right specific primers were designed when genomic sequence was available on either side of the insertion site. Right specific primers were designed when the insertion site defined novel maize genomic sequence. A presumptive right primer was designed for FSTs without a MuTIR sequence.