- Research article
- Open Access
Sequence-indexed mutations in maize using the UniformMu transposon-tagging population
- A Mark Settles1Email author,
- David R Holding2,
- Bao Cai Tan1,
- Susan P Latshaw1,
- Juan Liu3,
- Masaharu Suzuki1,
- Li Li1,
- Brent A O'Brien1,
- Diego S Fajardo1,
- Ewa Wroclawska1,
- Chi-Wah Tseung1,
- Jinsheng Lai4,
- Charles T HunterIII1,
- Wayne T Avigne1,
- John Baier1,
- Joachim Messing4,
- L Curtis Hannah1,
- Karen E Koch1,
- Philip W Becraft3,
- Brian A Larkins2 and
- Donald R McCarty1
© Settles et al; licensee BioMed Central Ltd. 2007
Received: 29 August 2006
Accepted: 09 May 2007
Published: 09 May 2007
Gene knockouts are a critical resource for functional genomics. In Arabidopsis, comprehensive knockout collections were generated by amplifying and sequencing genomic DNA flanking insertion mutants. These Flanking Sequence Tags (FSTs) map each mutant to a specific locus within the genome. In maize, FSTs have been generated using DNA transposons. Transposable elements can generate unstable insertions that are difficult to analyze for simple knockout phenotypes. Transposons can also generate somatic insertions that fail to segregate in subsequent generations.
Transposon insertion sites from 106 UniformMu FSTs were tested for inheritance by locus-specific PCR. We confirmed 89% of the FSTs to be germinal transposon insertions. We found no evidence for somatic insertions within the 11% of insertion sites that were not confirmed. Instead, this subset of insertion sites had errors in locus-specific primer design due to incomplete or low-quality genomic sequences. The locus-specific PCR assays identified a knockout of a 6-phosphogluconate dehydrogenase gene that co-segregates with a seed mutant phenotype. The mutant phenotype linked to this knockout generates novel hypotheses about the role for the plastid-localized oxidative pentose phosphate pathway during grain-fill.
We show that FSTs from the UniformMu population identify stable, germinal insertion sites in maize. Moreover, we show that these sequence-indexed mutations can be readily used for reverse genetic analysis. We conclude from these data that the current collection of 1,882 non-redundant insertion sites from UniformMu provide a genome-wide resource for reverse genetics.
An important aspect of functional genomics is understanding the phenotypic consequence of mutations in all genes in a genome. A comprehensive collection of gene knockouts allows a defined set of mutations to be systematically studied for more efficient association of genes to functions (reviewed in ). Multiple approaches have been used to develop comprehensive knockout resources. Biological differences between organisms make specific technologies such as homologous recombination, RNA interference, or insertional mutagenesis more useful in generating a resource for an individual species. In plants, a comprehensive knockout collection was generated for Arabidopsis thaliana via mutagenesis with insertion tags [2–5]. Genomic DNA flanking each tag was systematically amplified and sequenced from each mutant. These Flanking Sequence Tags (FSTs) index each mutant to the genome and are accessible through the SIGnAL T-DNA Express database, which links the mutant stocks to genome annotations . A similar FST approach was used to develop a rice functional genomics resource based on insertional mutagenesis populations [7–10]. The current rice collections have more than 140,000 insertion lines with associated FSTs that are integrated at the OryGenesDB database .
Maize is comparable to rice as a model grass species for genome studies. Similar to rice, the maize genome is now being sequenced with a minimal tiling path strategy . There are also extensive gene-enriched sequences that are estimated to include partial sequence from 95% of maize genes with smaller introns [12, 13]. In contrast to rice, maize is a monoecious plant, and maize has a shorter life cycle. These biological characteristics facilitate genetic analysis. Also, maize inbreds are highly polymorphic. Robust PCR markers, genetic maps, and recombinant inbred lines have been developed that aid quantitative trait studies and positional cloning (reviewed in ). A comprehensive knockout collection of maize mutants would complement the existing genome resources to make functional genomics studies in maize simple and rapid.
There are multiple insertion-tagged maize populations that were generated with either Activator (Ac) or Mutator (Mu) transposons (reviewed in ). There are >150,000 mutagenized lines among the combined Mu populations [16–20]. These Mu lines are expected to have more than 1.5 million independent insertions, because Mu transposons accumulate to high copy numbers within individual plants (reviewed in ). Moreover, Mu elements show a bias for inserting into or near transcribed regions of the genome and are associated with a high rate of forward mutagenesis [17, 19, 22, 23]. This high mutation frequency makes Mu mutagenesis attractive for generating knockout resources, but the high-copy nature of Mu elements presents a challenge in isolating individual insertion sites for sequencing. Single plants have multiple germinal insertions that represent both progenitor mutations and mutations unique to the individual. In addition, active transposon systems can generate somatic insertions that fail to segregate in subsequent generations. Due to these challenges, most Mu populations have been developed to conduct reverse genetics screens for only one gene at a time [16, 18, 20].
FSTs have been generated from two Mu populations. Fernandes et al.  identified 14,887 non-redundant FSTs using a transgenic Mu1 element that was engineered for plasmid rescue of genomic flanking sequences. The plasmids were isolated from pools of actively transposing plants and many of the FSTs are from somatic insertions. A key challenge to sequencing FSTs from pools of actively transposing plants is identifying germinal insertion sites and associating the germinal insertions to individual seed stocks. Fernandes et al.  sequenced from two dimensional grids. Recovery of the same FST from both row and column pools of plants was used to associate 528 of the plasmid-rescue insertion sites to individual plants.
In contrast, FSTs from the UniformMu population were generated with both a strategy to associate each FST to individual lines and to select for germinal insertions [19, 23]. UniformMu is a Mu population that is introgressed into the color-converted W22 inbred. Previously, we showed that UniformMu is a robust forward genetics resource for identifying visible mutant phenotypes . UniformMu FSTs were isolated by amplifying native transposon insertion sites with a modified TAIL-PCR protocol, MuTAIL . MuTAIL products were cloned from individual lines into small libraries. Sequencing randomly selected clones, similar to an EST strategy, recovers 67%–75% of the insertion sites from each line. With this sequencing strategy, each FST corresponds to an individual plant or maize ear.
The UniformMu FSTs also derive from plants in which the Mu transposons were stabilized by selecting against somatic transposition using the bronze1-mum9 (bz1-mum9) mutation [24, 25]. The selection against somatic activity predicts that all of the FSTs should be from germinal insertion sites. Database searches with 1,737 non-redundant UniformMu FSTs indicated a bias for insertions in or near transcribed regions of the genome. Moreover, in silico mapping of the FSTs indicated that UniformMu transposon insertions are distributed randomly throughout the maize chromosomes suggesting the transposon mutagenesis gave rise to mutations on a genome-wide basis. These properties predict that UniformMu FSTs will be a resource of sequence-indexed mutations that is simple to use for reverse genetics applications.
Here we test the utility of UniformMu FSTs for reverse genetics. We assayed a sample of FSTs for germinal segregation. We focused on FSTs that were predominantly recovered from individual UniformMu lines. FSTs found only in one plant have the highest possibility of representing somatic insertions and provide the strongest test for whether somatic insertions exist within the UniformMu FSTs. Our data indicate that 89% of the UniformMu FSTs can be confirmed as germinal insertions by designing only one or two locus-specific primers for PCR validation. The most common problems for the 11% of insertions that were not validated as germinal insertions were design errors in the locus-specific primers due to low quality sequence data or human error. One of the FSTs co-segregates with a seed mutant that suggests an important role for chloroplast-localized oxidative pentose phosphate pathway enzymes in grain-fill, embryogenesis, and plant development. These results demonstrate that the strategy for sequencing UniformMu FSTs creates effective reverse genetics resources for characterizing gene knockouts in maize.
A sample of 106 sequence-indexed insertion sites from the UniformMu population were tested for germinal inheritance. The UniformMu FSTs represent novel insertion sites from the individual lines as well as progenitor sequences that are found throughout the population. The FSTs initially were clustered by both blastcluster analysis  and CAP3 assembly to identify non-redundant sequences. A total of 1,434 FSTs unique to individual UniformMu seed mutants and 283 FSTs found in two to three mutants were identified with these clustering methods. We selected 79 FSTs found in individual mutants, 19 FSTs found in two mutants, and 8 FSTs found in multiple UniformMu lines for PCR validation. The 98 low redundancy insertions found in 1–2 mutants were recovered from 50 of the lines analyzed by MuTAIL sequencing. Thus, the 106 insertions sites tested were a significant sample of both UniformMu lines and MuTAIL FSTs.
Annotations of the insertion sites confirmed in Figures 2 and 3
No EST support for gene
plastid 6-phosphogluconate dehydrogenase
Probable prefoldin subunit 2
No EST support for gene
Putative ATP dependent copper transporter
Putative calmodulin-binding protein
Putative cytosolic chaperonin delta-subunit
No EST support for gene
SAM & endosperm expression
The FSTs were also analyzed for the presence of the conserved Mu Terminal Inverted Repeat (MuTIR). MuTAIL-PCR products are amplified with 29 bp of MuTIR sequence downstream of the transposon specific primer . Presence of the MuTIR sequence identifies the precise insertion site for a FST (see Table 1 and Additional file 1). MuTIR sequences were present in 91 FSTs and absent in the remaining 15 insertions sites selected for validation. MuTIR sequences are expected to be absent from a proportion of FSTs, because the MuTAIL products were cloned in random orientations and are frequently larger than single-pass sequence reads. Locus-specific PCR primers were designed based on the location of the MuTIR and MAGI sequence matches. We used three strategies to design the primers (Fig 1B). First, left and/or right primers were designed when the precise insertion site was placed within a MAGI sequence. The left primer was based on the MAGI sequence, and the right primer was designed using an alignment of the MAGI and FST. Second, a single right primer was designed when the MAGI showed partial overlap with the FST. These primers were designed using either an alignment between the FST and MAGI or were designed with the FST alone. Third, a presumptive right primer was designed for the 15 FSTs that did not have MuTIR sequences based on the hypothesis that these MuTAIL products were not completely sequenced.
Annotations of insertion sites that were not confirmed with PCR assays
Blast Cluster/Read Name
Likely cause for failure to amplify
repeat hit only
egg cell expressed
primer design error
No EST support for gene
primer design error
leaf & SAM expressed
No EST support for gene
primer design error
No EST support for gene
primer design error
low quality sequence
repeat hit only
Novel maize genomic sequence
low quality sequence
low quality sequence
repeat hit only
Novel maize genomic sequence
primer design error
26S protease regulatory subunit 7
cold stressed seedling expression
low quality sequence
6PGDH enzyme activity was measured from 25 days after pollination (DAP) endosperm extracts of W22, rgh*-00S-005-14 homozygous mutants as well as single and double pgd1; pgd2 mutants (Figure 4F). The W22 sample showed two major isozyme activities. The faster migrating activity corresponds to three bands: PGD1 homodimers, PGD1/PGD2 heterodimers, and PGD2 homodimers . A subset of these bands are absent in the pgd1 and pgd2 single mutants, and all three bands are lost in pgd1; pgd2 double mutant samples. The rgh mutant sample lacks the slower migrating activity. Serial dilutions of the W22 extract suggest that the rgh mutant endosperm has <5% normal activity for this 6PGDH isozyme (data not shown). We conclude the slower migrating 6PGDH isozyme is likely to be PGD3 as no reduction of this isozyme activity was observed in the other pgd mutants. These data suggest that the FST for pgd3-umu1 identified an enzymatic knockout.
The current maize knockout resources have enough insertions to disrupt every gene within the genome. FSTs will make these maize mutants far more available to the research community, and sequencing MuTAIL products is a high throughput approach to generate maize FSTs. However, FSTs alone do not make a reverse genetics resource easy to use. The tags need to have low entry barriers for tracking an insertion site of interest as well as for obtaining the line or strain that corresponds to the insertion site. FSTs from the UniformMu population are predicted to have low entry barriers due to the genetic markers included in the population and the high-throughput sequencing strategy used to generate the FSTs .
The bz1-mum9 mutant was used to select against somatic activity, and here we have shown that the vast majority of the resulting FSTs segregate as germinal insertions. We did not observe any evidence of somatic insertions. Somatic insertions would be expected to amplify from the DNA template used to generate the FSTs, but not to amplify from any other related individuals. In the cases where we could not confirm an insertion, the insertion site did not amplify from the template DNA used to generate the FSTs.
A secondary analysis of the specific primers indicated that three factors led to a small fraction of insertion sites that are more difficult to track. First, differences between available maize genomic sequences and the FSTs caused the precise insertion site to be mis-identified for 6.7% of the FSTs. These mis-identifications led to errors in locus-specific primer design. The sequence differences are likely to result from sequence errors, incomplete maize genomic sequence, and sequence divergence between the W22 inbred background used to develop UniformMu and the B73 inbred chosen for genomic sequencing. Comparative genomic sequence studies of maize inbreds suggests that there are significant differences in gene content and repetitive elements between inbreds [38–40]. A second factor that contributed to insertion sites that were not confirmed as germinal was human error in identifying the TIR-genomic DNA junction. These errors led to primer design errors for 1.9% of the FSTs. Finally, PCR or sample tracking errors occur at a low frequency in any high-throughput sequencing project. We speculate that a small amount of cross-contamination between sequencing libraries can explain the remaining 2.8% of insertion sites that were not confirmed. Importantly, 89% of the FSTs were readily confirmed as germinal insertions by locus-specific PCR. Furthermore, the MuTAIL FSTs were generated from individual plants or lines that were successfully bulked, and each sequence read name corresponds directly to an individual. These characteristics make it simple to identify the seed stocks for each FST. Combined, the characteristics of the MuTAIL FSTs and the UniformMu population make a robust resource for reverse genetics.
The pgd3-umu1 insertion site that co-segregates with a seed mutant phenotype illustrates the utility of the UniformMu FST resource for generating hypotheses about gene function. The pgd3-umu1 insertion is in a predicted chloroplast-localized 6PGDH enzyme in the oxidative section of the pentose phosphate pathway (OPPP). The linked rgh*-00S-005-14 seed mutant lacks a 6PGDH isozyme activity suggesting pgd3-umu1 causes an enzymatic knockout. These linkage and biochemical data suggest the hypothesis that Pgd3 has an essential role in seed development. Consistent with this hypothesis, the OPPP has been shown to be involved in hexose cycling and starch synthesis in developing seeds . Also, previous studies with maize pgd1 and pgd2 mutants argued that the plastidic enzymes for the oxidative section of the OPPP can compensate for loss of 6PGDH in the cytosol [29, 31]. Moreover, a study in maize root tips found that the oxidative section of the OPPP to be most active in plastids . Combined these data are consistent with plastidic OPPP enzymes being required for seed development. However, linkage data alone cannot prove that the rgh phenotype is a result of the pgd3-umu1 mutation. Additional experiments are necessary to test this hypothesis such as identifying multiple mutant alleles of Pgd3. Alternatively, a Pgd3 transgene could be developed to test for complementation of the 6PGDH enzyme defect and the rgh*-00S-005-14 phenotype. The maize genetics community has multiple resources to assist in identifying alleles or in generating transgenics for individual genes of interest (reviewed in ).
The pgd3-umu1 example also illustrates the complementary nature of having reverse genetics resources in multiple plant species. The Arabidopsis genome contains two copies of genes predicted to encode plastid-localized 6PGDH enzymes and one gene predicted to encode a cytosolic 6PGDH (Figure 5; ). Each of these genes have multiple sequence-indexed mutations that disrupt exon sequences and are likely to cause null alleles . Gene redundancy in Arabidopsis may be one reason why mutant phenotypes associated with plastid-localized 6PGDH have not been reported previously. If plastidic 6PGDH enzymes are essential in plants, a double mutant for knockouts of Arabidopsis orthologs, At1g64190 and At5g41670, would be expected to show a visible phenotype.
The rice genome has a single locus that is predicted to encode plastid-localized 6PGDH, Os11g29400, similar to maize. However, the current rice knockout resources do not have FSTs that disrupt this locus . The rice ortholog for cytosolic 6PGDH, Os06g02144, has three insertions, but these insertions map to promoter and 3' UTR regions and may not be null alleles. Thus, the maize mutants in 6PGDH enzymes suggest an agricultural relevance for the plastid-localized OPPP that would not be as simple to identify using the current Arabidopsis or rice resources.
The pgd3-umu1 allele is just one example of the current collection of 1,882 non-redundant insertion sites sequenced in UniformMu. We conclude from our analysis that the vast majority of the UniformMu insertion sites are germinal. Importantly, multiple studies have shown that Mu insertions are heavily biased for transcribed regions of the genome [17, 19, 22, 23]. In addition, the UniformMu population is in an inbred background that simplifies phenotypic analysis . Combined, these features provide a robust, genome-wide resource for reverse genetics in maize. Further sequencing of UniformMu FSTs would enhance this resource by expanding it towards a comprehensive knockout collection.
The UniformMu FSTs were clustered onto maize sequences using the methods described in McCarty et al. . In addition, the FSTs were assembled using CAP3 , and the FST contigs corresponding to the blastclusters chosen for PCR analysis were retrieved from the assembly for more detailed annotation. The FST contigs were queried against the MAGI4.0 release of the maize genome survey sequence assembly as well as release 16.0 of the Zea mays gene index (ZMGI) using BLASTN searches [27, 28, 45]. Top matches were visually inspected to determine if the sequence alignment was consistent with a precise match to the maize genome or tentative consensus EST contig. An insertion site was considered precisely identified when the top match had >95% identity between the FST and the MAGI or ZMGI. For matches to MAGI sequences, alignments were expected to extend through the full length of the MAGI or FST sequence with no insertion or deletion polymorphisms >20 bp. When a FST showed significant identity with more than one MAGI, the MAGI sequence that defined the precise insertion site was reported. The MAGI sequence matching each FST was also queried against the ZMGI to identify MAGIs with evidence for a transcribed region.
Each FST was scored for the presence of the MuTIR by visual inspection of the terminal end sequence of the FST contigs. Insertion site positions were classified as follows. Promoter insertions had TIR-genomic DNA junctions that were <850 bp 5' of the ZMGI tentative consensus EST contig. Exon insertions had a TIR junction within a ZMGI sequence. Intron insertions had a TIR junction in genomic DNA that could be positively identified as intron sequence based on an alignment of the FST, MAGI, and ZMGI sequences. Unknown insertions did not have a ZMGI match with either the FST or corresponding MAGI sequence. FSTs without a clear TIR sequence were also classified as unknown.
The Pgd3 locus was assembled by aligning the bc199 FST contig with MAGI4_45584, MAGI4_45583, and the ZMGI TC300585 contig. The largest open reading frame (ORF) was selected and used to generate the protein similarity tree with ClustalW and TreeDraw, which were run on the SDSC Biology Workbench [34, 46, 47].
The PCR assays were completed with multiple methods, and no significant difference was observed between approaches. Detailed methods for specific insertion sites can be obtained from the primary contacts given in Additional file 2 for each insertion site and primer. In general, DNA was extracted as described in Settles, et al. . The locus-specific primers were designed with Primer3 for 20–25 base oligomers with a predicted Tm between 60–65°C . The specific primers were paired with a MuTIR specific primer. MuTIR primers used in this study included TIR4, TIR6, and TIR8 from Settles, et al.  as well as the primers 5'-CGCCTCYATTTCGTCGAATCC-3' and 5'-GCCTCCATTTCGTCGAATC-3'. PCR was typically completed with Taq DNA polymerase and buffers from either Invitrogen or Promega with either 5% DMSO or 1 M Betaine added to the final reaction. Thermocycling conditions were generally 94°C for 1 minute, 60°C for 1 minute, 72°C for 1 minute for 40 cycles. Annealing temperatures were adjusted when the predicted Tm of a specific primer was lower than 60°C. Most specific primers gave products in the first set of PCR conditions tested, and a careful study of optimal general methods for amplifying specific insertions sites was not completed.
rgh*-00S-005-14 Mutant Phenotypic Analysis
The longitudinal hand sections of mutant and normal rgh kernels were completed with mature kernels from a self-pollinated heterozygous individual. The kernels were imbibed in water overnight and sectioned with a fresh razor blade and unstained sections were imaged. To recover homozygous mutant plants, rgh mutant and normal seed were removed from mature, segregating self-pollinations (30–35 DAP) prior to drying the ears. The seed were surface-sterilized in 70% ethanol for 2 min, transferred to 20% bleach, 0.1% Tween 20 for 15 min, and washed in sterile water four times. The pericarp was removed from the kernels under sterile conditions and transferred to Murashige and Skoog salt and vitamin mixture at pH 5.7 with 0.4% Phytagel and 1% sucrose. The seeds were cultured at 32/25°C (day/night) with a 16-h photoperiod. Germinated seedlings were transferred to soil after 2–4 weeks of culture, and grown in greenhouse conditions with a similar temperature and light regime.
6PGDH enzyme activity was measured by native-PAGE assays essentially as described by Bailey-Serres et al . Briefly, 0.1 g of endosperm tissue was ground in extraction buffer (20 mM Tris, pH 8.0, 4 mM DTT, 5 mM MgCl2, 15% (v/v) glycerol, 0.001% Bromophenol blue) at a ratio of 0.2 mL extraction buffer to 0.1 g tissue fresh weight. The protein extracts were cleared prior to gel loading by centrifugation at 10,000× g for 10 min at 4°C. The extracts were loaded based on equal fresh weight of starting samples and the proteins were separated at 4°C with native-PAGE (7% acrylamide, 0.375 M Tris, pH 8.8, 12% glycerol(v/v)). After electrophoresis, the gel was stained for 6PGDH (0.1 M Tris-HCl, pH 7.5, 0.1 mg/mL NADP, 0.1 mg/mL nitro blue tetrazolium, 0.01 mg/mL phenazine methosulfate, 0.5 mg/mL 6-phosphogluconate) at room temperature for 30 min. The gel was de-stained with water prior to drying and digital imaging.
The authors thank Julia Bailey-Serres for providing homozygous mutant seeds for the pgd1 and pgd2 single mutants as well as the pgd1; pgd2 double mutant. The authors also thank the anonymous reviewers for critical reading of the manuscript. This research was supported with funding from the NSF Plant Genome Research Program (grant #0076700), the Vasil-Monsanto Endowment, and the University of Florida Genetics Institute Seed Grant Program.
- Carpenter AE, Sabatini DM: Systematic genome-wide screens of gene function. Nature Reviews Genetics. 2004, 5 (1): 11-22. 10.1038/nrg1248.PubMedView ArticleGoogle Scholar
- Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen HM, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR: Genome-wide Insertional mutagenesis of Arabidopsis thaliana. Science. 2003, 301 (5633): 653-657. 10.1126/science.1086391.PubMedView ArticleGoogle Scholar
- Rosso MG, Li Y, Strizhov N, Reiss B, Dekker K, Weisshaar B: An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics. Plant Molecular Biology. 2003, 53 (1): 247-259. 10.1023/B:PLAN.0000009297.37235.4a.PubMedView ArticleGoogle Scholar
- Samson F, Brunaud V, Duchene S, De Oliveira Y, Caboche M, Lecharny A, Aubourg S: FLAGdb(++): a database for the functional analysis of the Arabidopsis genome. Nucleic Acids Res. 2004, 32: D347-D350. 10.1093/nar/gkh134.PubMed CentralPubMedView ArticleGoogle Scholar
- Sessions A, Burke E, Presting G, Aux G, McElver J, Patton D, Dietrich B, Ho P, Bacwaden J, Ko C, Clarke JD, Cotton D, Bullis D, Snell J, Miguel T, Hutchison D, Kimmerly B, Mitzel T, Katagiri F, Glazebrook J, Law M, Goff SA: A high-throughput Arabidopsis reverse genetics system. Plant Cell. 2002, 14 (12): 2985-2994. 10.1105/tpc.004630.PubMed CentralPubMedView ArticleGoogle Scholar
- SIGnAL T-DNA Express. [http://signal.salk.edu/cgi-bin/tdnaexpress]
- An GH, Lee S, Kim SH, Kim SR: Molecular genetics using T-DNA in rice. Plant and Cell Physiology. 2005, 46 (1): 14-22. 10.1093/pcp/pci502.PubMedView ArticleGoogle Scholar
- Kolesnik T, Szeverenyi I, Bachmann D, Kumar CS, Jiang S, Ramamoorthy R, Cai M, Ma ZG, Sundaresan V, Ramachandran S: Establishing an efficient Ac/Ds tagging system in rice: large-scale analysis of Ds flanking sequences. Plant Journal. 2004, 37 (2): 301-314.PubMedView ArticleGoogle Scholar
- Kuromori T, Hirayama T, Kiyosue Y, Takabe H, Mizukado S, Sakurai T, Akiyama K, Kamiya A, Ito T, Shinozaki K: A collection of 11,800 single-copy Ds transposon insertion lines in Arabidopsis. Plant Journal. 2004, 37 (6): 897-905. 10.1111/j.1365.313X.2004.02009.x.PubMedView ArticleGoogle Scholar
- Yazaki J, Kojima K, Suzuki K, Kishimoto N, Kikuchi S: The Rice PIPELINE: a unification tool for plant functional genomics. Nucleic Acids Res. 2004, 32: D383-D387. 10.1093/nar/gkh001.PubMed CentralPubMedView ArticleGoogle Scholar
- Droc G, Ruiz M, Larmande P, Pereira A, Piffanelli P, Morel JB, Dievart A, Courtois B, Guiderdoni E, Perin C: OryGenesDB: a database for rice reverse genetics. Nucleic Acids Res. 2006, 34: D736-D740. 10.1093/nar/gkj012.PubMed CentralPubMedView ArticleGoogle Scholar
- Bruggmann R, Bharti AK, Gundlach H, Lai JS, Young S, Pontaroli AC, Wei FS, Haberer G, Fuks G, Du CG, Raymond C, Estep MC, Liu RY, Bennetzen JL, Chan AP, Rabinowicz PD, Quackenbush J, Barbazuk WB, Wing RA, Birren B, Nusbaum C, Rounsley S, Mayer KFX, Messing J: Uneven chromosome contraction and expansion in the maize genome. Genome Research. 2006, 16 (10): 1241-1251. 10.1101/gr.5338906.PubMed CentralPubMedView ArticleGoogle Scholar
- Springer NM, Xu XQ, Barbazuk WB: Utility of different gene enrichment approaches toward identifying and sequencing the maize gene space. Plant Physiology. 2004, 136 (2): 3023-3033. 10.1104/pp.104.043323.PubMed CentralPubMedView ArticleGoogle Scholar
- Bortiri E, Jackson D, Hake S: Advances in maize genomics: the emergence of positional cloning. Current Opinion in Plant Biology. 2006, 9 (2): 164-171. 10.1016/j.pbi.2006.01.006.PubMedView ArticleGoogle Scholar
- Settles AM: Maize community resources for forward and reverse genetics. Maydica. 2005, 50: 405-414.Google Scholar
- Bensen RJ, Johal GS, Crane VC, Tossberg JT, Schnable PS, Meeley RB, Briggs SP: Cloning and Characterization of the Maize An1 Gene. Plant Cell. 1995, 7 (1): 75-84. 10.1105/tpc.7.1.75.PubMed CentralPubMedView ArticleGoogle Scholar
- Fernandes J, Dong QF, Schneider B, Morrow DJ, Nan GL, Brendel V, Walbot V: Genome-wide mutagenesis of Zea mays L. using RescueMu transposons. Genome Biology. 2004, 5 (10): R82-10.1186/gb-2004-5-10-r82.PubMed CentralPubMedView ArticleGoogle Scholar
- May BP, Liu H, Vollbrecht E, Senior L, Rabinowicz PD, Roh D, Pan XK, Stein L, Freeling M, Alexander D, Martienssen R: Maize-targeted mutagenesis: A knockout resource for maize. Proceedings of the National Academy of Sciences of the United States of America. 2003, 100 (20): 11541-11546. 10.1073/pnas.1831119100.PubMed CentralPubMedView ArticleGoogle Scholar
- McCarty DR, Settles AM, Suzuki M, Tan BC, Latshaw S, Porch T, Robin K, Baier J, Avigne W, Lai JS, Messing J, Koch KE, Hannah LC: Steady-state transposon mutagenesis in inbred maize. Plant Journal. 2005, 44 (1): 52-61.PubMedView ArticleGoogle Scholar
- Stern DB, Hanson MR, Barkan A: Genetics and genomics of chloroplast biogenesis: maize as a model system. Trends in Plant Science. 2004, 9 (6): 293-301. 10.1016/j.tplants.2004.04.001.PubMedView ArticleGoogle Scholar
- Walbot V, Rudenko GN: MuDR/Mu transposable elements in maize. Mobile DNA II. Edited by: Craig NL, Craigie M, Gellert AM, Lambowitz AM. 2002, Washington, D.C. , ASM Press, 533-564.View ArticleGoogle Scholar
- Cresse AD, Hulbert SH, Brown WE, Lucas JR, Bennetzen JL: Mu1-Related Transposable Elements of Maize Preferentially Insert into Low Copy Number DNA. Genetics. 1995, 140 (1): 315-324.PubMed CentralPubMedGoogle Scholar
- Settles AM, Latshaw S, McCarty DR: Molecular analysis of high-copy insertion sites in maize. Nucleic Acids Res. 2004, 32 (6): e54-10.1093/nar/gnh052.PubMed CentralPubMedView ArticleGoogle Scholar
- Brown J, Sundaresan V: Genetic-Study of the Loss and Restoration of Mutator Transposon Activity in Maize - Evidence against Dominant-Negative Regulator Associated with Loss of Activity. Genetics. 1992, 130 (4): 889-898.PubMed CentralPubMedGoogle Scholar
- Brown WE, Robertson DS, Bennetzen JL: Molecular Analysis of Multiple Mutator-Derived Alleles of the Bronze Locus of Maize. Genetics. 1989, 122 (2): 439-445.PubMed CentralPubMedGoogle Scholar
- Huang XQ, Madan A: CAP3: A DNA sequence assembly program. Genome Research. 1999, 9 (9): 868-877. 10.1101/gr.9.9.868.PubMed CentralPubMedView ArticleGoogle Scholar
- Fu Y, Emrich SJ, Guo L, Wen TJ, Ashlock DA, Aluru S, Schnable PS: Quality assessment of maize assembled genomic islands (MAGIs) and large-scale experimental verification of predicted genes. Proceedings of the National Academy of Sciences of the United States of America. 2005, 102 (34): 12282-12287. 10.1073/pnas.0503394102.PubMed CentralPubMedView ArticleGoogle Scholar
- Quackenbush J, Cho J, Lee D, Liang F, Holt I, Karamycheva S, Parvizi B, Pertea G, Sultana R, White J: The TIGR Gene Indices: analysis of gene transcript sequences in highly sampled eukaryotic species. Nucleic Acids Res. 2001, 29 (1): 159-164. 10.1093/nar/29.1.159.PubMed CentralPubMedView ArticleGoogle Scholar
- Averill RH, Bailey-Serres J, Kruger NJ: Co-operation between cytosolic and plastidic oxidative pentose phosphate pathways revealed by 6-phosphogluconate dehydrogenase-deficient genotypes of maize. Plant Journal. 1998, 14 (4): 449-457. 10.1046/j.1365-313X.1998.00143.x.View ArticleGoogle Scholar
- MaizeGDB. [http://www.maizegdb.org/]
- Bailey-Serres J, Tom J, Freeling M: Expression and Distribution of Cytosolic 6-Phosphogluconate Dehydrogenase Isozymes in Maize. Biochemical Genetics. 1992, 30 (5-6): 233-246.PubMedView ArticleGoogle Scholar
- Kruger NJ, von Schaewen A: The oxidative pentose phosphate pathway: structure and organisation. Current Opinion in Plant Biology. 2003, 6 (3): 236-246. 10.1016/S1369-5266(03)00039-6.PubMedView ArticleGoogle Scholar
- Krepinsky K, Plaumann M, Martin W, Schnarrenberger C: Purification and cloning of chloroplast 6-phosphogluconate dehydrogenase from spinach - Cyanobacterial genes for chloroplast and cytosolic isoenzymes encoded in eukaryotic chromosomes. European Journal of Biochemistry. 2001, 268 (9): 2678-2686. 10.1046/j.1432-1327.2001.02154.x.PubMedView ArticleGoogle Scholar
- Thompson JD, Higgins DG, Gibson TJ: Clustal-W - Improving the Sensitivity of Progressive Multiple Sequence Alignment through Sequence Weighting, Position-Specific Gap Penalties and Weight Matrix Choice. Nucleic Acids Res. 1994, 22 (22): 4673-4680. 10.1093/nar/22.22.4673.PubMed CentralPubMedView ArticleGoogle Scholar
- Emanuelsson O, Nielsen H, Brunak S, von Heijne G: Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. Journal of Molecular Biology. 2000, 300 (4): 1005-1016. 10.1006/jmbi.2000.3903.PubMedView ArticleGoogle Scholar
- Nakai K, Horton P: PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization. Trends in Biochemical Sciences. 1999, 24 (1): 34-35. 10.1016/S0968-0004(98)01336-X.PubMedView ArticleGoogle Scholar
- Small I, Peeters N, Legeai F, Lurin C: Predotar: A tool for rapidly screening proteomes for N-terminal targeting sequences. Proteomics. 2004, 4 (6): 1581-1590. 10.1002/pmic.200300776.PubMedView ArticleGoogle Scholar
- Brunner S, Fengler K, Morgante M, Tingey S, Rafalski A: Evolution of DNA sequence nonhomologies among maize inbreds. Plant Cell. 2005, 17 (2): 343-360. 10.1105/tpc.104.025627.PubMed CentralPubMedView ArticleGoogle Scholar
- Song RT, Messing J: Gene expression of a gene family in maize based on noncollinear haplotypes. Proceedings of the National Academy of Sciences of the United States of America. 2003, 100 (15): 9055-9060. 10.1073/pnas.1032999100.PubMed CentralPubMedView ArticleGoogle Scholar
- Fu HH, Dooner HK: Intraspecific violation of genetic colinearity and its implications in maize. Proceedings of the National Academy of Sciences of the United States of America. 2002, 99 (14): 9573-9578.PubMed CentralPubMedView ArticleGoogle Scholar
- Ettenhuber C, Spielbauer G, Margl L, Hannah LC, Gierl A, Bacher A, Genschel U, Eisenreich W: Changes in flux pattern of the central carbohydrate metabolism during kernel development in maize. Phytochemistry. 2005, 66 (22): 2632-2642. 10.1016/j.phytochem.2005.09.017.PubMedView ArticleGoogle Scholar
- Dieuaide-Noubhani M, Raffard G, Canioni P, Pradet A, Raymond P: Quantification of Compartmented Metabolic Fluxes in Maize Root-Tips Using Isotope Distribution from C-13-Labeled or C-14-Labeled Glucose. Journal of Biological Chemistry. 1995, 270 (22): 13147-13159. 10.1074/jbc.270.22.13147.PubMedView ArticleGoogle Scholar
- The Arabidopsis Information Resource. [http://www.arabidopsis.org/]
- OryGenesDB. [http://orygenesdb.cirad.fr]
- Altschul SF, Madden TL, Schaffer AA, Zhang JH, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25 (17): 3389-3402. 10.1093/nar/25.17.3389.PubMed CentralPubMedView ArticleGoogle Scholar
- Felsenstein J: Phylogeny Inference Package (Version 3.2). Cladistics. 1989, 5: 164-166.Google Scholar
- SDSC Biology WorkBench. [http://workbench.sdsc.edu/]
- Rozen S, Skaletsky HJ: Primer3 on the WWW for general users and for biologist programmers. Bioinformatics Methods and Protocols: Methods in Molecular Biology. Edited by: Krawetz S, Misener S. 2000, Totowa, New Jersey , Humana Press, 365-386.Google Scholar
- UniformMu Maize Population. [http://www.uniformmu.org]
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.