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Table 2 Annotations of insertion sites that were not confirmed with PCR assays

From: Sequence-indexed mutations in maize using the UniformMu transposon-tagging population

Blast Cluster/Read Name MAGI4.0 hit Insertion site ZMGI hit Insertion type Annotation/Expression Pattern Likely cause for failure to amplify
03S-0467-13A2-G01 repeat hit only   DR906457 exon egg cell expressed primer design error
bc0041 MAGI4_104 252 no hit   No EST support for gene primer design error
bc0093 MAGI4_65387 128 DN229926 exon leaf & SAM expressed unknown
bc0182 MAGI4_121677 no MuTIR no hit   No EST support for gene primer design error
bc1042 MAGI4_128194 1502 no hit   No EST support for gene primer design error
bc1073 MAGI4_94877 3164 TC303220 exon TM20/Dek34 gene low quality sequence
bc1382 repeat hit only no MuTIR no hit   Novel maize genomic sequence low quality sequence
bc1435 MAGI4_132661 no MuTIR DT943333 unknown thioesterase superfamily low quality sequence
bc1509 repeat hit only   no hit   Novel maize genomic sequence primer design error
bc1526 MAGI4_13062 no MuTIR TC292893 unknown 26S protease regulatory subunit 7 unknown
bc1537 MAGI4_5849 481 TC291433 intron cold stressed seedling expression low quality sequence
bc1571 MAGI4_154704 734 TC282381 -40bp short-chain dehydrogenase/reductase unknown