Figure 1
Distribution analysis of homoeologous segments in tetraploid cotton by FISH. (A) Figure showed that the identification of the BAC clone 75F07 (lane 5) containing the polymorphic locus of NAU837-205, and 68D15 (lane 6) containing the monomorphic locus NAU837-195 between TM-1 and Hai7124 by SSR marker NAU837. Lanes 1–4 were Hai7124, F1(TM-1 × Hai7124), TM-1 and restorer line 0-613-2R, respectively. (B) FISH image showed that the signals of the polymorphic allele BAC 75F07 (green signals, arrows) and chromosome A6-specific BAC 47N15 (red signals, arrowheads) were located on the same chromosome. It indicated that the polymorphic allele BAC 75F07 derived from chromosome A6. (C) FISH image showed that the BAC clone 68D15 (red signals, arrowheads) located on the same chromosome with chromosome-specific BAC clone 24K19 (green signals, arrows) of chromosome D6.