- Research article
- Open Access
Detection and mapping of homologous and homoeologous segments in homoeologous groups of allotetraploid cotton by BAC-FISH
© Wang et al; licensee BioMed Central Ltd. 2007
- Received: 19 December 2006
- Accepted: 19 June 2007
- Published: 19 June 2007
Cotton, as an allopolyploid species, contains homoeologous A and D subgenomes. The study of the homoeologous (duplicated) segments or chromosomes can facilitate insight into the evolutionary process of polyploidy and the development of genomic resources. Fluorescence in situ hybridization (FISH) using bacterial artificial chromosome (BAC) clones as probes has commonly been used to provide a reliable cytological technique for chromosome identification. In polyploids, it also presents a useful approach for identification and localization of duplicated segments. Here, two types of BACs that contained the duplicated segments were isolated and analyzed in tetraploid cotton by FISH.
Homologous and homoeologous BACs were isolated by way of SSR marker-based selection and then used to develop BAC-FISH probes. Duplicated segments in homoeologous chromosomes were detected by FISH. The FISH and related linkage map results followed known reinforced the relationships of homoeologous chromosomes in allotetraploid cotton, and presented a useful approach for isolation of homoeologous loci or segments and for mapping of monomorphic loci. It is very important to find that the large duplicated segments (homologous BACs) do exist between homoeologous chromosomes, so the shot-gun approach for genome sequencing was unavailable for tetraploid cotton. However, without doubt, it will contain more information and promote the research for duplicated segments as well as the genome evolution in cotton.
These findings and the analysis method by BAC-FISH demonstrated the powerful nature and wide use for the genome and genome evolutionary researches in cotton and other polyploidy species.
- Bacterial Artificial Chromosome
- Bacterial Artificial Chromosome Clone
- Bacterial Artificial Chromosome Library
- Homoeologous Chromosome
- Duplicate Segment
Polyploidy is an evolutionary process whereby two or more genomes are brought together in the same nucleus, usually by hybridization followed by chromosome doubling . Accordingly, most of these genomes contain duplicated chromosomes or chromosomal segments that reflect ancient or recent rounds of polyploidy. Therefore, investigations of the levels of diversity and patterns of duplicated genes and segments in polyploid plants can provide insights into the process of polyploidization and subsequent processes. When genes are duplicated as a consequence of polyploidization, they may continue to evolve at the same rate as they did in their diploid ancestors, or they may be subject to pressures that lead to differential rates of sequence evolution . Ultimately, these duplicated sequences and their functions are maintained intact or undergo long-term evolutionary change via sequence elimination [3, 4], sequence rearrangement , gene silencing , or acquisition of new function . Most of the evolution process of large segments or genome is accompanied with the duplicated genes evolution or operates organizational level of duplicated genes. The process of polyploidy evolution leading to stabilization and species formation have been studied and confirmed by modern molecular genetic techniques [3, 8–11]. A new phenomenon occurring after polyploidy was found by analysing the evolution of dispersed repeats, and the results showed that there has been substantial colonization of the D genome by A genome repetitive elements [10, 12]. The various aspects of genome evolution involving duplicated sequences in polyploids have been reviewed elsewhere .
Cotton (Gossypium) is particularly useful for studies of polyploidy . A simple method for isolating homoeologous loci from allopolyploids has been developed in cotton . In addition, investigations of duplicated genes have revealed their evolutionary rate , patterns and levels of nucleotide diversity [16–18], and functional silencing  exerted polyploidy. Nevertheless, the relatively few examples studied to date provide little understanding of genomic evolution after duplication. In fact, reports on evolutionary rate have been contradictory [15, 17], probably due to limited sampling.
In this study, different types of duplicated segments-containing bacterial artificial chromosomes (BACs) were isolated. Fluorescence in situ hybridization (FISH), performed with a complete set of chromosome-specific BAC clones developed in tetraploid cotton , discriminated the duplicated segments locating on homoeologous chromosomes. The results present new evidence for tetraploid cotton homoeologous chromosomes relationship as well as a new approach to the isolation of homoeologous loci or segments. And these also provided a new chance for the research of duplicated sequences in cotton.
Homoeologous BACs derived from homoeologous chromosomes
Distribution analysis of homoeologous SSR markers
Genetic mapping of polymorphic loci
Monomorphic loci BACs
BAC end primers
Physical location of monomorphic loci BACs
Linkage mapping of BACs
Polymorphic primers and corresponding BAC end sequences used in this study
GenBank accession number of end sequences
Homologous BACs derived from homoeologous chromosomes
BAC-FISH of another BAC clone, 68O15, further supported these findings. This BAC was isolated using NAU2195-200, which mapped on chromosome D12. Like BAC 09D09, it also generated two pairs of bright signals on two pairs of different chromosomes. Repeated-FISH with A12 and D12 chromosome-specific BACs, 43C02 and 10G17, found that these two pairs of signals originated from the corresponding homoeologous chromosomes A12 and D12 (figures not shown).
Identification and analysis of homoeologous chromosome BACs
Tetraploid cotton (n = 2x = 26, AD) was derived from two diploids with A and D genomes that diverged from a common ancestor . Therefore, in theory, there should be 13 pairs of homoeologous chromosomes in tetraploid cotton. Recently, by distribution analysis of duplicated marker loci among chromosomes, all 13 pairs of homoeologous chromosomes have been discriminated [24, 25]. Here, these findings of duplicated segments derived from BACs provide new evidence for homoeologous chromosome discrimination. As shown in Figure 1A and 1B, two BACs derived from the monomorphic and polymorphic loci of one marker were identified on the A6/D6 homoeologous chromosomes which had been identified based on the similar phenotypes of monosomic plants  and duplicated molecular loci. For each BAC, the same primer origin indicated the existence of homologous sequences, and the distribution of duplicated segments confirmed the homoeologous relationships. So it was considered as well homoeologous segments (BACs) as new evidences for homoeologous chromosome detection.
This finding could be applied to the high-density physical map construction of the cotton genome. As demonstrated above, the two bands (two loci) produced by marker NAU837 which likely derived from the homology between different homoeologous chromosomes in allotetraploid cotton. Therefore, one of this kind of markers could be used to screen two groups of BACs that are located on one pair of homoeologous chromosomes. In fact, it effectively increased by one fold the number of BACs for physical map construction. It is particularly useful since this kind of BAC can not be isolated using the molecular loci on a genetic map due to the monomorphism between mapping parents. Therefore, these BACs are needed for the continued construction of the physical map and for further physical map based-genome sequencing. It has been used to help isolate cotton BACs for contigs construction in our lab. Furthermore, the genetic location of BAC-ends sequences presents a test for the location of monomorphic locus BACs. On the other hand, it also demonstrated an alternative mapping and researching approach for this kind of monomorphic locus which usually occurred in genetic marker and could not mapped by the means of genetic mapping. It makes the further researches of this kind of duplicated loci possible, and the collinearity analysis of duplicated loci produced by some EST-derived SSR also has been carried out in our lab to discover the evolution of duplicated genes in tetraploid cotton.
Cotton is a naturally occurring polyploid with eight diploid genomes distributed over four continents . And it has become a useful model system for the study of the genome and molecular evolution of allopolyploids, especially with the modern molecular evolution researches on duplicated loci [15, 16, 27–29]. Here, our finding provided a novel approach for duplicated loci isolation that avoids the likelihood of errors from PCR amplification . More importantly, due to the normal phenomenon of markers like NAU837 in tetraploid cotton, the large scale isolation of duplicated loci is much simpler. The isolation and testing of a sufficient number of duplicated loci will unravel some previous contradictories [15, 17, 18] and provide the basis for phylogenetic analysis of lineages. And the finding of duplicated segments in BACs would also provide new challenges in the study of cotton genome evolution by analyzing its distribution, maintenance mechanisms during long periods of diploidization, and diversity following polyploidization.
Homologous BAC identification and analysis
Another case showed as BAC 09D09 is similar to the duplicated molecular loci derived from one molecular marker, and duplicated segments (loci) also discriminated the homoeologous relationship in tetraploid cotton. However, unlike the duplicated molecular loci, most of which are no more than 1 kb, BAC-FISH reveals a much larger segment (~100 kb) of homeology. Therefore, they are more reasonable for finding homoeologous relationships . Several cases were found in which two pairs of signals were detected, but usually one pair of signals was brighter and the other was too weak to be located by multi-FISH. However, one pair of chromosomes was always clearly larger than the other, indicating that they should belong to different subgenomes. Furthermore, the several kb of DNA sequence in FISH detectable signals suggests that the homologous segments likely originate from the homoeologous chromosomes. Therefore, the possibility is raised that in any particular case, the FISH signal derived from a BAC in a different subgenome may reinforce the homoeologous relationships in tetraploid cotton rather than random repetitive-sequence or reciprocal or non-reciprocal translocations.
In comparison with the homeology between the two BACs derived from marker NAU837, which may be just several hundreds of base pairs due to SSR amplification, the duplicated segments originating from BAC 09D09 may be more than 100 kb. Furthermore, the equal strength of the two pairs of FISH signals is indicative of a high level of homology between the segments in the A and D subgenomes. If this degree of homology is the norm, shotgun sequencing is not feasible for tetraploid cotton because the assemblage of such large scale homology would be impossible. With the rapid development of the cotton genetic map and BAC library construction, genome sequencing based on the physical map may prove to be preferred choice for cotton.
Nevertheless, the case of BAC 09D09 is an excellent example of homoeologous analysis because the length of the duplicated segment must be as long as several scores of kilobase pairs due to the bright FISH signals detected. It will, no doubt, provide more information for evolutionary analysis of duplications at the chromosomal or genomic levels. The distribution of BAC 09D09 in the A-genome and not the D-genome of diploid cotton indicated that it originated in the A-genome ancestor and spread to the D-subgenome after polyploidy. This phenomenon was previously described by Hanson et al. (1998)  and Zhao et al. (1998)  from the analysis of distributions of dispersed repetitive DNA in cotton. And since polyploidization there has been substantial colonization of the D genome by A genome repetitive elements . Our results reinforce the model that A-genomic DNA "infected" the D-subgenome, with homoeology occurring subsequent to polyploidization. However, these findings also point out that large DNA segments do spread to new subgenomes and are maintained during long period of evolution in allotetraploid cotton. It is a very different pattern in DNA content and component from the repetitive DNA theory, so the most probable mechanism may be inter-genomic recombination and exchange between subgenomes in the nonfunctional segments. Because the segments are so large, it is impossible for them to behave as a functional gene or transposon to "jump" between different subgenomes. The concept that pairing and recombination between homoeologous chromosomes does not appear to have substantially affected the organization of the modern cotton genome, as determined by analysis of molecular markers repetitive DNA [12, 31], would be novel and contrary to current belief. This novel concept might also provide new opportunities to understand the tetraploid cotton genome and its evolutionary process.
Finally, BAC-FISH mapping presents a powerful tool for evolution research in tetraploid cotton. Previous studies have shown that a correlation exists between chromosomal position and levels of genetic diversity at a locus . However, researchers can not analyze the diversity level of genes or sequences by combining the genetic and physical data because it is not possible to physically map short duplicated genes or sequences, and only the genetic position allows them to reference and speculate in their analysis . We are currently able to correlate these genetic mapping data with physical chromosomal locations constructed by BAC-FISH. Therefore, analysis of the relationship between genetic diversity and chromosomal position will become available for tetraploid cotton.
In conclusion, BAC-FISH as a new approach for the duplicated segments analysis and physical mapping of monomorphic locus in cotton were presented. Two types of BACs containing duplicated segments were found and physically mapped. The results showed that they were derived from the homoeologous chromosomes. According to the degree of duplicated segment containing in BACs, they were named homologous and homoeologous BACs. Analysis of these BACs presents us some new approach for the researches of cotton genome and genome evolution. Especially, the large duplicated segments (homologous BACs) were identified by FISH, and it demonstrated the powerful potential for the research of cotton genome evolution. In a word, these findings are just an entrance and a challenge for the cotton genome researches.
The BACs used in this study were found in two genomic BAC libraries derived from the tetraploid cotton strain TM-1 and 0-613-2R, a restorer line of cytoplasmic male sterility . The TM-1 library was kindly supplied by Dr. John Yu, USDA-ARS, Crops Germplasm Research Unit, College Station, Texas. TM-1 is a highly inbred line of G. hirsutum L. (2n = 52). The A and D subgenome chromosomes were renamed A1 through A13 and D1 though D13, respectively, based on new tetraploid cotton nomenclature for homoeologous chromosomes .
Only TM-1 mitotic metaphase chromosomes were used for FISH. Root tip, slide preparation, and single-color FISH have been described previously . Dual-color FISH was performed as in Ji et al. (1997)  with some modifications. Purified BAC DNA was labelled with biotin-16-dUTP or digoxigenin-11-dUTP by the BioNick Labelling System (Roche Diagnostics, Mannheim, Germany). Following post-hybridization washes, signals from the digoxigenin-labelled probes were detected by anti-digoxigenin-rhodamine, and directly from biotin-labelled probes by avidin-fluorescein. DAPI (4', 6-diamidino-2-phenylindole) (Sigma, St. Louis, MO) in an antifade solution, Vectashield (Vector, Burlingame, CA) was used to counterstain the chromosomes. Slides were examined under an Olympus BX51 fluorescence microscope. Chromosome and FISH signal images were captured using an Evolution VF CCD camera (Media Cybernetics, Silver Spring, MD) and merged using Image-Pro Express software.
BAC-end sequencing was performed by National Center for Gene Research, CAS, China. And the online software Primer3 was used to design the BAC-specific primers. Loci were genetic mapped on the genetic map by combination the recombination data among the BC1 population with the new map data .
This work was financially supported in part by grants from the program of Changjiang Scholars and Innovative Research Team in University of MOE, China. We would like to thank Dr. J Yu, USDA-ARS, Crops Germplasm Research Unit, Texas, USA for supplying us with BAC clones from the TM-1 library.
- Levy AA, Feldman M: The impact of polyploidy on grass genome evolution. Plant Physiol. 2002, 130: 1587-1593. 10.1104/pp.015727.PubMed CentralPubMedView ArticleGoogle Scholar
- Wendel JF: Genome evolution in polyploids. Plant Mol Biol. 2000, 42: 225-249. 10.1023/A:1006392424384.PubMedView ArticleGoogle Scholar
- Feldman M, Liu B, Segal G, Abbo S, Levy AA: Rapid elimination of low-copy DNA sequences in polyploid wheat: A possible mechanism for differentiation of homoeologous chromosomes. Genetics. 1997, 147: 1381-1387.PubMed CentralPubMedGoogle Scholar
- Liu B, Vega JM, Segal G, Abbo S, Rodova M, Feldman M: Rapid genomic changes in newly synthesized amphiploids of Triticum and Aegilops. I. Changes in low-copy noncoding DNA sequences. Genome. 1998, 41: 272-277. 10.1139/gen-41-2-272.View ArticleGoogle Scholar
- Kenton A, Parokonny AS, Gleba YY, Bennett MD: Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics. Mol Gen Genet. 1993, 240: 159-169. 10.1007/BF00277053.PubMedView ArticleGoogle Scholar
- Zhu T, Schupp JM, Oliphant A, Keim P: Hypomethylated sequences: characterization of the duplicate soybean genome. Mol Gen Genet. 1994, 244: 638-645. 10.1007/BF00282754.PubMedView ArticleGoogle Scholar
- Ohta T: Multigene families and the evolution of complexity. J Mol Evol. 1991, 33: 34-41.7. 10.1007/BF02100193.PubMedView ArticleGoogle Scholar
- Brubaker CL, Paterson AH, Wendel JF: Comparative genetic mapping of allotetraploid cotton and its diploid progenitors. Genome. 1999, 42: 184-203. 10.1139/gen-42-2-184.View ArticleGoogle Scholar
- Cheun WY, Champagne G, Hubert N, Landry BS: Comparison of the genetic maps of Brassica napus and Brassica oleracea. Theor Appl Genet. 1997, 94: 569-582. 10.1007/s001220050453.View ArticleGoogle Scholar
- Zhao XP, Si Y, Hanson RE, Crane CF, Price HJ: Dispersed repetitive DNA has spread to new genomes since polyploid formation in cotton. Genome Res. 1998, 8: 479-492.PubMedGoogle Scholar
- Song K, Lu P, Tang K, Osborn TC: Rapid genome change in synthetic polyploids of Brassica and its implications for polyploid evolution. Proc Natl Acad Sci USA. 1995, 92: 7719-7723. 10.1073/pnas.92.17.7719.PubMed CentralPubMedView ArticleGoogle Scholar
- Hanson RE, Zhao XP, Islam-Faridi MN, Paterson AH, Zwick MS, Crane CF, McKnight TD, Stelly DM, Price HJ: Evolution of interspersed repetitive elements in Gossypium (Malvaceae). Am J Bot. 1998, 85: 1364-1368. 10.2307/2446394.PubMedView ArticleGoogle Scholar
- Wendel JF, Cronn RC: polyploidy and the evolutionary history of cotton. Adv Agron. 2003, 78: 139-186.View ArticleGoogle Scholar
- Cronn RC, Wendel JF: Simple methods for isolating homoeologous loci from allopolyploid genomes. Genome. 1998, 41: 756-762. 10.1139/gen-41-6-756.View ArticleGoogle Scholar
- Cronn RC, Small RL, Wendel JF: Duplicated genes evolve independently after polyploid formation in cotton. Proc Natl Acad Sci USA. 1999, 96 (25): 14406-14411. 10.1073/pnas.96.25.14406.PubMed CentralPubMedView ArticleGoogle Scholar
- Small RL, Wendel JF: Copy number lability and evolutionary dynamics of the Adh gene family in diploid and tetraploid cotton (Gossypium). Genetics. 2000, 155: 1913-1926.PubMed CentralPubMedGoogle Scholar
- Small RL, Wendel JF: Differential evolutionary dynamics of duplicated paralogous Adh loci in allotetraploid cotton (Gossypium). Mol Biol Evol. 2002, 19: 597-607.PubMedView ArticleGoogle Scholar
- Small RL, Ryburn JA, Wendel JF: Low levels of nucleotide diversity at homoeologous Adh loci in allotetraploid cotton (Gossypium L.). Mol Biol Evol. 1999, 16: 491-501.PubMedView ArticleGoogle Scholar
- Adams KL, Cronn R, Percifield R, Wendel JF: Genes duplicated by polyploidy show unequal contributions to the transcriptome and organ-specific reciprocal silencing. Proc Natl Acad Sci USA. 2003, 100: 4649-4654. 10.1073/pnas.0630618100.PubMed CentralPubMedView ArticleGoogle Scholar
- Wang K, Song XL, Han ZG, Guo WZ, Yu JZ, Sun J, Pan JJ, Kohel RJ, Zhang TZ: Complete assignment of the chromosomes of Gossypium hirsutum L. by translocation and fluorescence in situ hybridization mapping. Theor Appl Genet. 2006, 113: 73-80. 10.1007/s00122-006-0273-7.PubMedView ArticleGoogle Scholar
- Wang K, Guo WZ, Zhang TZ: Development of one set of chromosome-specific microsatellite-containing BACs and their physical mapping in Gossypium hirsutum L. Theor Appl Genet. 2007,Google Scholar
- Han ZG, Wang CB, Song XL, Guo WZ, Guo JY, Li CH, Chen XY, Zhang TZ: Characteristics, development and mapping of G. hirsutum derived-EST-SSRs in allotetraploid cotton. Theor Appl Genet. 2006, 112: 430-439. 10.1007/s00122-005-0142-9.PubMedView ArticleGoogle Scholar
- Wendel JF: New World tetraploid cottons contain Old World cytoplasm. Proc Natl Acad Sci USA. 1989, 86: 4132-4136. 10.1073/pnas.86.11.4132.PubMed CentralPubMedView ArticleGoogle Scholar
- Nguyen TB, Giband M, Brottier P, Risterucci AM, Lacape JM: Wide coverage of the tetraploid cotton genome using newly developed microsatellite markers. Theor Appl Genet. 2004, 109: 167-175. 10.1007/s00122-004-1612-1.PubMedView ArticleGoogle Scholar
- Rong JK, Abbey C, Bowers JE, Brubaker CL, Chang C, Chee PW, Delmonte TA, Ding X, Garza JJ, Marler BS, Park C, Pierce GJ, Rainey KM, Rastogi VK, Schulze SR, Trolinder NL, Wendel JF, Wilkins TA, Williams-Coplin TD, Wing RA, Wright RJ, Zhao X, Zhu L, Paterson AH: A 3347-locus genetic recombination map of sequence-tagged sites reveals features of genome organization, transmission and evolution of cotton (Gossypium). Genetics. 2004, 166: 389-417. 10.1534/genetics.166.1.389.PubMed CentralPubMedView ArticleGoogle Scholar
- Endrizzi JE, Ramsay G: Monosomes and telosomes for 18 of 26 chromosomes of Gossypium hirsutum. Can J Genet Cytol. 1979, 21: 531-536.View ArticleGoogle Scholar
- Wendel JF, Schnabel A, Seelanan T: Bidirectional interlocus concerted evolution following allopolyploid speciation in cotton (Gossypium). Proc Natl Acad Sci USA. 1995, 92: 280-284. 10.1073/pnas.92.1.280.PubMed CentralPubMedView ArticleGoogle Scholar
- Cronn RC, Zhao X, Paterson AH, Wendel JF: Polymorphism and concerted evolution in a tandemly repeated gene family: 5S ribosomal DNA in diploid and allopolyploid cottons. J Mol Evol. 1996, 42: 685-705. 10.1007/BF02338802.PubMedView ArticleGoogle Scholar
- Liu B, Brubaker CL, Mergeai G, Cronn RC, Wendel JF: Polyploid formation in cotton is not accompanied by rapid genomic changes. Genome. 2001, 44: 321-330. 10.1139/gen-44-3-321.PubMedView ArticleGoogle Scholar
- Blake TK, Kadyrzhanova D, Shepherd KW, Islam AKMR, Langridge PL, Mcdonald CL, Erpelding J, Larson S, Blake NK, Talbert LE: STS-PCR markers appropriate for wheat-barley introgression. Theor Appl Genet. 1996, 93: 826-832. 10.1007/BF00224082.PubMedView ArticleGoogle Scholar
- Reinisch AJ, Dong JM, Brubaker CL, Stelly DM, Wendel JF, Paterson AH: A detailed RFLP map of cotton (Gossypium hirsutum × Gossypium barbadense): chromosome organization and evolution in a disomic polyploid genome. Genetics. 1994, 138: 829-847.PubMed CentralPubMedGoogle Scholar
- Begun DJ, Aquadro CF: Levels of naturally occurring DNA polymorphism correlate with recombination rates in D. melanogaster . Nature. 1992, 356: 519-520. 10.1038/356519a0.PubMedView ArticleGoogle Scholar
- Yin JM, Guo WZ, Zhang TZ: Construction of BAC library for 0-613-2R in Upland cotton. J Integr Plant Biol. 2006, 48: 219-222.View ArticleGoogle Scholar
- Ji Y, Raska DA, McKnight TD, Islam-Faridi NM, Crane CF, Zwick MS, Hanson RE, Price HJ, Stelly DM: Use of meiotic fluorescence in situ hybridization for identification of a new monosome in Gossypium hirsutum L. Genome. 1997, 40: 34-40.PubMedView ArticleGoogle Scholar
- Guo WZ, Cai CP, Wang CB, Han ZG, Song XL, Wang K, Niu XW, Wang C, Lu KY, Shi B, Zhang TZ: A microsatellite-based, gene-rich linkage map reveals genome structure, function, and evolution in Gossypium. Genetics. 2007, 176 (1): 527-541. 10.1534/genetics.107.070375.PubMed CentralPubMedView ArticleGoogle Scholar
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