From: A DArT platform for quantitative bulked segregant analysis
 |  |  |  |  |  | Linkage-detection threshold (p < 0.05)5 | |
---|---|---|---|---|---|---|---|
Bulk size | Number of replicate arrays | Marker-selection p threshold1 | Minimum hybridization difference between parents2 | Number of polymorphic markers identified3 | Markers previously mapped in Steptoe/Morex population4 | Based on 'platform noise'6 | Based on 'pooling noise'7 |
20 | 4 | 0.001 | 0.88 | 515 | 257 | 26% | 50% |
20 | 4 | 0.0001 | 1.04 | 433 | 231 | 24% | 50% |
20 | 4 | 0.00001 | 1.18 | 384 | 211 | 23% | 50% |
20 | 2 | 0.0001 | 1.06 | 356 | 187 | 24% | 50% |
20 | 8 | 0.0001 | 0.66 | 669 | 294 | 24% | 50% |
40 | 4 | 0.0001 | 1.04 | 418 | 221 | 23% | 37.5% |