Table 4 Primers used for construction of DIG-labelled DNA probes
From: Living with an imperfect cell wall: compensation of femAB inactivation in Staphylococcus aureus
Primer | Sequence (5'-3') |
|---|---|
dltA- F | TCAGGCGGTACATTAAATCTTGT |
dltA- R | TATGTGTTGTAAATCGTCGCACT |
dnaK-F | CGATGAGCCAAAAGTAATTC |
dnaK-R | TACTTCGAATACACCGTCAC |
fmtC-F | CCGTATGTCCTTAGTGTTAC |
fmtC-R | GCAGTACAATCCTACAAAAC |
glnA-F | AGATGGAACACCATTTGAAG |
glnA-R | AAACGTTAAAGTGCATACCG |
glpF-F | TAGACGGAAGTTTTGATTGG |
glpF-R | GGCAATTGGTCCTAAGATAG |
opuCC-F | TTGTCGTGTTTGTCTTATCG |
opuCC-R | ACGTATTCGCAAAACCATAC |
pyrP-F | TTATCACGGGATTAAGTACG |
pyrP-R | ACAATCGGAATCATTACAAG |
pyrR-F | AACGTACAGTGACGAGAATC |
pyrR-R | TAACTGCATTTCTTTGATCC |
RNAII-F | CGAAGACGATCCAAAAC |
RNAII-R | TTATCTAAATGGGCAATGAGT |
RNAIII-F | GTGATGGAAAATAGTTGATGAG |
RNAIII-R | GTGAATTTGTTCACTGTGTCG |
sa1007-F | TAATGAATCCTGTCGCTAAT |
sa1007-R | TTCAGTGTATGACCAATCGAA |
saeR-F | GACCCACTTACTGATCGTG |
saeR-R | CCTAATCCCCATACAGTTGTG |
sarA-F | AGGGAGGTTTTAAACATGGC |
sarA-R | CTCGACTCAATAATGATTCG |
splA-F | GAATTACCTGGTTGTGCATACG |
splA-R | GAAGACCTTGCGATAGTTCATG |