Table 7 Primer sequences used for PCRs
From: Gene profile analysis of osteoblast genes differentially regulated by histone deacetylase inhibitors
| Gene | Strand | Primer Sequences | Annealing Temp (°C) | Product Length (bp) |
|---|---|---|---|---|
| Akap12 | F | 5' GCCAGTGAAGAACATGAGCAG | 56 | 160 |
| R | 5' CTATGCAATCTGCTTTGTCTTGG | |||
| Ap4s1 | F | 5' GTACTTCAGCCGAGTGAGTG | 56 | 202 |
| R | 5' CTCTTCCTTGGCCTTCACAG | |||
| Amphiregulin | F | 5' GACTCACAGCGAGGATGACAAG | 56 | 249 |
| R | 5' GCTTGGCAATGATTCAAC | |||
| Fibroblast growth factor 7 | F | 5' CAGTTTGGAAAGAGCGACGAC | 56 | 170 |
| R | 5' GGCAGGATCCGTGTCAGTATC | |||
| Frizzled-1 | F | 5' CAAGGTTTACGGGCTCATGTAC | 56 | 180 |
| R | 5' GTAACAGCCGGACAGGAAAATG | |||
| Frizzled-4 | F | 5' CTGCAGCATGCCTAATGAGAG | 56 | 185 |
| R | 5' CGTCTGCCTAGATGCAATCA | |||
| Interleukin 1 receptor-like 1 | F | 5' CCAGCCAGAGTGGAAGACTC | 56.9 | 189 |
| R | 5' CAGGTCAATTGTTGGACACG | |||
| Psmb10 | F | 5' CTTTACTGCCCTTGGCTCTG | 56.9 | 168 |
| R | 5' GTGATCACACAGGCATCCAC | |||
| Qpct | F | 5' GAGGAGGCACTGAAGGAGTG | 53 | 162 |
| R | 5' GAAGATCGGACTGGATGCTC | |||
| Sdh1 | F | 5' CTGCCGATTCTACAAGCACA | 56.9 | 183 |
| R | 5' AGCAAAGTGACCATCCCAAC | |||
| Slc9a3r1 | F | 5' CAAAGTGTCCCCTCACCAGT | 56.9 | 209 |
| R | 5' AATGAACCCAAGATGCCAAG |
