Table 1 Screening of the BAC library with 24 microsatellites located on each of the 24 linkage groups of L. calcarifer
Linkage group | Locus | GenBank accession no | Forward primer (5'-3') | Reverse primer (5'-3') | PCR Ta (°C) | Positive clone number |
|---|---|---|---|---|---|---|
LG1 | Lca318 | DQ290175 | TCCCACCCCAGTCCAGAAA | TACCAGAGCCTGAAACACAGTAGG | 55 | 6 |
LG2 | Lca064 | AY998856 | AGGCATATGCACCTCACAAGAGTG | CCCACGGTTTATTTATCTGTCATTATC | 55 | 15 |
LG3 | Lca137 | DQ290039 | CGCCTTAAATCTCTACGCTCTGG | TCGCATGCTGTAATTAAGGTGGTA | 55 | 5 |
LG4 | Lca171 | DQ290065 | ATTGCGTTACCAAGAGGTGAA | TGTCTTTGAAGGCTGAAAACTG | 55 | 8 |
LG5 | Lca098 | AY998880 | CAAAGGGGCCACTGCACATAAT | CTCCAGCTCACCCAGGTTCACT | 55 | 5 |
LG6 | Lca062 | AY998854 | AGGATGGCACGCTGAAACTATCG | ATAAGCTTGTACAGGGGCTGAGTGC | 55 | 3 |
LG7 | Lca130 | DQ290035 | GAGGCTCCCAATCCCAACAA | GGAGGCAGACGAGGAAGGAA | 55 | 13 |
LG8 | Lca086 | AY998873 | AAATGGCCTTCCTGTCCCTTCAG | GTGTTCCCTTGTTCTGCCACAGTG | 55 | 4 |
LG9 | Lca301 | DQ290166 | GCCAGTGTGAGGGACAGAGA | GGGCCTTGTTTTGCTTTTG | 55 | 9 |
LG10 | Lca002 | AF007943 | GCCGCTTGTTTACCAGTAAA | TCCATTTGAGGATTAACAGC | 55 | 5 |
LG11 | Lca058 | AY998850 | AAACAGGCAGCCAGATAGACAGAG | AAGAGGTGGTGGGACTAATTTGAGA | 55 | 13 |
LG12 | Lca074 | AY998863 | CATCATTTACACTCTGTTTGCCTCAT | GACAGACAGGTGTTTTAGCCTATTTG | 55 | 6 |
LG13 | Lca253 | DQ290129 | TGGGGACTTGACTTCCTTTTATG | TACCGAGGTTGGATGGTTTTCT | 55 | 3 |
LG14 | Lca147 | DQ290047 | TGCCCCTAATGTATTCTTTCCACT | GCTCCCACCTCTCATTCATTATTC | 55 | 5 |
LG15 | Lca069 | AY998859 | GCCTTTCTGTTTTCTGATTTATCTTCAT | AACACCCCGAAATACTGCTACTACAG | 55 | 4 |
LG16 | Lca367 | DQ290206 | TGTATTACAATGCCCGTGGTCA | TTAAGCCTTTGGTGTCTCAGTGTG | 55 | 10 |
LG17 | Lca021 | AF404083 | GTGCCACCTGCCTGACC | GCCATGACTGATTGCTGAGA | 55 | 4 |
LG18 | Lca193 | DQ290082 | CCTCTGCCTTTTCATCTATATTGC | CACATCGCACAAATGGACTGA | 55 | 9 |
LG19 | Lca220 | DQ290104 | ATGGCTGTGAAAAGACTGGTATCT | CGCCCCTCACTCAACAGAG | 55 | 5 |
LG20 | Lca181 | DQ290073 | CACTGGGTGGCGTTTGTATTAGC | CAAGAATTGGGATTTTGCTGTGC | 55 | 8 |
LG21 | Lca255 | DQ290131 | AGAGACACTTTATACGGGGACATC | GTAAACTGAAGCAAGCCAAACCT | 55 | 7 |
LG22 | Lca040 | AF404099 | TGAGGAAGCATCAGCTGTAATCA | CAGGACGCAAACACTGAAAT | 55 | 3 |
LG23 | Lca411 | DQ290221 | GTGGTGCAGCGGTTGCTCTC | CCGACTCATGCTGCTTTTCGTAAT | 55 | 5 |
LG24 | Lca231 | DQ290112 | GGCCAGGTTAATCAAGAC | ACTAGACTGCAATCAAACACA | 55 | 3 |