Table 2 Screening of the BAC library with 15 genes/ESTs of L. calcarifer
Locus | GenBank accession no | Primer (5'-3', forward) | Primer (5'-3', reverse) | PCR Ta (°C) | PCR product length (bp) | Positive clone number |
|---|---|---|---|---|---|---|
PVALB-1 | AY688372 | ATCGTCCGTCCGTTTCCCATAAAA | TGACCTTTCACCTCCCTCCAGACC | 55 | 261 | 5 |
5-HT | EU136181 | CTGCTCGGCGCGCTCAT | TCCATCCTGCACCTGTGCG | 60 | 200 | 8 |
PROL-A | EU136180 | GTGCAGAGCCGTCSCCATCA | TTCAGGAAGCTGTCRATCTTGTG | 55 | 500 | 5 |
14KDA-AP | EU136179 | CCGGGGACAGACAACTCGCTTTCAGAGA | ACAGGTTGGTGAGCTCCAGTTGGTGTTC | 55 | 500 | 4 |
AMY-A | AY007592 | GGTCGCTTTCCGTAATGTGGTCAA | ACCGGGCATGCCAGTGTTCA | 55 | 250 | 9 |
MX | Ay821518 | TCATTGATAAAGTGACAGCATTCA | CCAATATCCTTGAGTTTCTTGACA | 55 | 400 | 7 |
AP | AJ888375 | GACGCCCTCCTCTCCTCTCA | TTTCGACAGCCATCTCTGAACATA | 55 | 700 | 4 |
LECT2 | EU136177 | TTTTTGATCTGAAGATGAGACGTGTCATC | GATCAGATCCCGAGCAGGTCAATC | 55 | 1000 | 3 |
LYSO-G | EU136178 | AGAGTCCAGGGCTGGAAAT | CCCTCAGAAACTTTAGTTGTGAAC | 55 | 600 | 9 |
IGF-1 | EU136176 | CAGTGGCATTTATGTGATGTC | CCTCGACTTGAGTTTTTCTG | 55 | 503 | 3 |
TUB1A | EU136175 | GGCACTACACAATCGGCAAAGAGA | TCAGCAGGGAGGTAAAGCCAGAGC | 55 | 144 | 11 |
TUB2B | EU136174 | GTACAGACGGGGGAAGGGGACCAT | TTCCGCACCCTCAAACTCACCACA | 55 | 160 | 13 |
GT7 | EU136172 | CAGGGTGATCACGCAGTGC | GGCAATCCGACAGCCAGAG | 55 | 156 | 6 |
CYP19A2 | AY684259 | GCTCACCGCCTATAGCCAAAGAA | GGCCGAGTCCTGCCAAGAAA | 55 | 505 | 8 |
AFPII | EU136173 | TCCCTCCTGTGAAATTGGTTGG | AGGGACGCTGGCACAGACTG | 57 | 1500 | 14 |