Figure 2

PCR amplification of 41 selected NumtS. PCR amplification of (a) 27 selected NumtS in 4 healthy subjects from different ethnic groups (haplogroups H2b (Europe), L2a1c1 (North Africa), I3a (Latin America) and G1a1a (Japan)); (b) 14 in the H2b sample only. Primers were designed with PRIMER3 software, testing the amplification of the full NumtS (external-external primers) or part of it (external-internal primers or internal-internal primers). In NumtS 41–54, samples H2b and L2a1c1 have shorter amplicons, due to a lower number of repetitions. Triple band in NUMTS 12 was due to aspecific amplification, subsequently reduced by increasing stringency of primers annealing. Abbreviations: ext for external, int for internal; the number below each band refers to the NumtS code assigned within the RHNumtS compilation.