Skip to main content

Chloroplast DNA sequence of the green alga Oedogonium cardiacum (Chlorophyceae): Unique genome architecture, derived characters shared with the Chaetophorales and novel genes acquired through horizontal transfer



To gain insight into the branching order of the five main lineages currently recognized in the green algal class Chlorophyceae and to expand our understanding of chloroplast genome evolution, we have undertaken the sequencing of chloroplast DNA (cpDNA) from representative taxa. The complete cpDNA sequences previously reported for Chlamydomonas (Chlamydomonadales), Scenedesmus (Sphaeropleales), and Stigeoclonium (Chaetophorales) revealed tremendous variability in their architecture, the retention of only few ancestral gene clusters, and derived clusters shared by Chlamydomonas and Scenedesmus. Unexpectedly, our recent phylogenies inferred from these cpDNAs and the partial sequences of three other chlorophycean cpDNAs disclosed two major clades, one uniting the Chlamydomonadales and Sphaeropleales (CS clade) and the other uniting the Oedogoniales, Chaetophorales and Chaetopeltidales (OCC clade). Although molecular signatures provided strong support for this dichotomy and for the branching of the Oedogoniales as the earliest-diverging lineage of the OCC clade, more data are required to validate these phylogenies. We describe here the complete cpDNA sequence of Oedogonium cardiacum (Oedogoniales).


Like its three chlorophycean homologues, the 196,547-bp Oedogonium chloroplast genome displays a distinctive architecture. This genome is one of the most compact among photosynthetic chlorophytes. It has an atypical quadripartite structure, is intron-rich (17 group I and 4 group II introns), and displays 99 different conserved genes and four long open reading frames (ORFs), three of which are clustered in the spacious inverted repeat of 35,493 bp. Intriguingly, two of these ORFs (int and dpoB) revealed high similarities to genes not usually found in cpDNA. At the gene content and gene order levels, the Oedogonium genome most closely resembles its Stigeoclonium counterpart. Characters shared by these chlorophyceans but missing in members of the CS clade include the retention of psaM, rpl32 and trnL(caa), the loss of petA, the disruption of three ancestral clusters and the presence of five derived gene clusters.


The Oedogonium chloroplast genome disclosed additional characters that bolster the evidence for a close alliance between the Oedogoniales and Chaetophorales. Our unprecedented finding of int and dpoB in this cpDNA provides a clear example that novel genes were acquired by the chloroplast genome through horizontal transfers, possibly from a mitochondrial genome donor.


The Chlorophyceae (sensu Mattox and Stewart) is a morphologically diverse class of green algae, which together with three other green algal classes (Prasinophyceae, Trebouxiophyceae and Ulvophyceae) form the Chlorophyta [1]. The Chlorophyceae and Ulvophyceae are presumed to share a sister-relationship, with the Trebouxiophyceae being sister to the Chlorophyceae + Ulvophyceae clade and the Prasinophyceae representing the earliest offshoot of the Chlorophyta [2, 3]. Members of the Chlorophyceae display the most variability in terms of the arrangement of the flagellar apparatus. The flagellar basal bodies of motile cells (vegetative cells, zoospores or gametes) are generally directly opposed (DO) or displaced in a clockwise (CW) direction [4]; however, those of the chlorophyceans assigned to the order Oedogoniales display a unique arrangement characterized by an anterior ring of flagella [5]. Phylogenetic analyses of 18S rDNA data and combined 18S and 26S rDNA data from a broad range of chlorophyceans uncovered five major monophyletic groups in the Chlorophyceae: the Chlamydomonadales, Sphaeropleales, Oedogoniales, Chaetophorales, and Chaetopeltidales. The interrelationships of these chlorophycean lineages, however, could not be unraveled. Although most internal nodes in the trees inferred from the 18S and 26S rDNA data received poor support, the five lineages were most often recovered as a grade, with the sister-relationship observed for the Chlamydomonadales and Sphaeropleales being the best supported [69].

Comparative analyses of chloroplast genomes have proven very useful to clarify the evolutionary relationships among the main groups of green algae and land plants [1018]. We adopted this strategy to gain insight into the branching order of chlorophycean lineages and also to better understand the forces accounting for the very fluid structure of the chloroplast genome in the Chlorophyceae. We recently described the complete chloroplast genomes of Scenedesmus obliquus (Sphaeropleales) [19] and Stigeoclonium helveticum (Chaetophorales) [20] and compared them to their homologue in Chlamydomonas reinhardtii (Chlamydomonadales) [21]. All three genomes have retained only a few of the ancestral characters observed in their counterparts from other classes. Their reduced gene repertoires, which comprise 94 to 97 genes, lack six of the protein-coding genes (accD, chlI, minD, psaI, rpl19, and ycf20) identified in Pseudendoclonium akinetum and Oltmannsiellopsis viridis, the two members of the Ulvophyceae whose chloroplast genomes have been examined so far [12, 22]. Some genes show unique alterations at the structural level. In all three chlorophyceans, the rpoB gene is split into two distinct open reading frames (ORFs) and the clpP and rps3 genes have unusually large coding regions. Moreover, the coding region of rps4 in Stigeoclonium displays a prominent insertion that is apparently devoid of any intron or intein. Both the Chlamydomonas and Scenedesmus genomes possess a large inverted repeat (IR) encoding the rRNA operons; however, the gene contents of their single copy (SC) regions are entirely different and do not conform to the ancestral quadripartite structure observed in the prasinophycean Nephroselmis olivacea [23] and in most streptophyte green algae [11, 16, 24, 25] and land plants [26, 27]. Despite these differences, they share 11 gene clusters that have not been previously observed in other green algae. The IR-lacking chloroplast genome of Stigeoclonium is extremely rearranged relative to its chlorophycean counterparts and exhibits a number of distinctive traits, including four putatively trans-spliced group II introns inserted in petD, psaC and rbcL as well as a remarkably strong bias in gene coding regions and base composition of the two DNA strands. Both the strand biases in coding regions and G + C composition were found to be typical of those observed in prokaryotic genomes that replicate bidirectionally from a single origin.

More recently, we used the sequence data of the three abovementioned chlorophycean chloroplast genomes together with those of the partly sequenced chloroplast DNAs (cpDNAs) of Chlamydomonas moewusii (Chlamydomonadales), Oedogonium cardiacum (Oedogoniales) and Floydiella terrestris (Chaetopeltidales) to reconstruct trees from nucleotide and amino acid data sets derived from more than 40 protein-coding genes [15]. All best trees identified two robustly supported lineages within the Chlorophyceae: a clade uniting the Chlamydomonadales and Sphaeropleales (CS clade) and a clade uniting the Oedogoniales, Chaetophorales, and Chaetopeltidales (OCC clade). This dichotomy was independently supported by molecular signatures in chloroplast genes such as insertions/deletions and the distribution of trans-spliced group II introns [15]. Within the OCC clade, the sister relationship observed for the Chaetophorales and Chaetopeltidales was strengthened by the presence of trans-spliced group II introns at two identical positions in the Stigeoclonium and Floydiella rbcL genes. Nevertheless, the possibility that the Chaetophorales are sister to the Oedogoniales + Chaetopeltidales clade could not be rejected by statistical tests. Character state reconstruction of basal body orientation using the best tree as phylogenetic framework predicted that the last common ancestor of all chlorophycean green algae featured quadriflagellate motile cells with the DO + DO orientation and that changes to the CW condition occurred convergently in the CS and OCC clades [15].

We present here the complete chloroplast genome sequence of Oedogonium (Oedogoniales) and report additional genomic characters bolstering the evidence for a close alliance between the Oedogoniales and Chaetophorales. Like its Stigeoclonium, Chlamydomonas and Scenedesmus homologues, the Oedogonium genome displays a distinctive architecture. Although this IR-containing chloroplast genome is highly shuffled in gene order relative to the other completely sequenced chlorophycean genomes, it shares unique gene clusters with the Stigeoclonium genome as well as the retention and loss of specific genes. To our surprise, we identified three genes that were acquired by horizontal gene transfer in a 10-kb region of the exceptionally large IR.


Overall structure and gene arrangement

The Oedogonium chloroplast genome assembles as a circle of 196,547 bp with an A+T content of 70.5% (Fig. 1). As shown in Table 1, these values fall within the range of sizes and base compositions observed for the three previously sequenced chlorophycean cpDNAs (Table 1). The Oedogonium genome displays two identical copies of a 35,492-bp IR that are separated from one another by SC regions of 80,363 and 45,200 bp, designated here SC1 and SC2, respectively. Note that the latter regions are not designated as large and small SC (LSC and SSC) regions as is the case for Nephroselmis and streptophyte cpDNAs because their gene contents deviate considerably from the ancestral partitioning pattern featured by these genomes [23, 28]. In addition, the quadripartite structure of the Oedogonium genome differs greatly from those of its Chlamydomonas and Scenedesmus homologues with respect to the gene contents of the IR and the SC regions and also the relative sizes of these three main genomic regions (Table 1). In Oedogonium, the IR is significantly larger than most of its green algal and land plants counterparts and the vastly unequal sizes of the SC regions contrast with the similar sizes of the same regions in Chlamydomonas and Scenedesmus cpDNAs.

Figure 1

Gene map of Oedogonium cpDNA. Thick lines on the inner circle represent the two copies of the IR (IRA and IRB), with the arrows indicating the transcription direction of the IR-encoded rRNA operon. Genes (filled boxes) on the outside of the outer circle are transcribed in a clockwise direction. Introns are represented by open boxes and intron ORFs are denoted by narrow, filled boxes. The rpoB gene consists of two separate ORFs (rpoBa and rpoBb) that are not associated with sequences typical of group I or group II introns. tRNA genes are indicated by the one-letter amino acid code followed by the anticodon in parentheses (Me, elongator methionine; Mf, initiator methionine). Only one of the two isomeric forms of the genome is shown here; these isomers differ with respect to the relative orientation of the SC regions.

Table 1 General features of Oedogonium and other chlorophycean cpDNAs

The 99 conserved genes and four free-standing ORFs of more than 100 codons found in Oedogonium cpDNA are densely packed (Fig. 1). The intergenic spacers account for 22.6% of the total genome sequence and vary from 22 to 1721 bp, for an average size of 370 bp. This is the lowest proportion and smallest average size of intergenic spacers observed thus far for the chloroplast genome of a photosynthetic chlorophyte belonging to the Ulvophyceae, Trebouxiophyceae or Chlorophyceae (UTC) (Table 1 and [29]).

The Oedogonium IR contains six conserved genes and three ORFs in addition to the rRNA-encoding operon, whereas the SC1 and SC2 regions encode 54 and 34 genes, respectively. The SC1 and SC2 boundaries of the IR lie in the 3'ends of ccsA and rpoA, respectively. Interestingly, the 3'end of trnR(ucu) encompasses the SC1 boundary of the IR; the last two bp of this gene coding region maps to the IR and are thus shared with ccsA. These genes were considered as part of the IR in the breakdown of genes provided above. The rRNA operon lies at the proximity of the SC2 region and is transcribed toward to the largest SC region (SC1). In contrast, the rRNA operon is always transcribed toward the smallest SC region in cpDNAs that have retained the ancestral quadripartite structure. In this context, it is worth mentioning that the chloroplast genome of the ulvophycean Pseudendoclonium, which carries an atypical quadripartite structure, resembles its Oedogonium homologue in exhibiting an IR whose rRNA operon is transcribed toward the largest SC region [12]. Considering that the latter region in Pseudendoclonium is clearly equivalent to the LSC region found in genomes having an ancestral quadripartite structure, it was speculated that a change in the transcription direction of the rRNA operon might have occurred in conjunction with the exchange of a few genes between the two SC regions. Subsequent analysis of an ulvophycean belonging to a separate early-branching lineage (Oltmannsiellopis) suggested that these genomic events might have occurred before or soon after the emergence of the Ulvophyceae [22]. Unlike their ulvophycean homologues, the Oedogonium SC regions display no remnant of an ancestral gene partitioning pattern, nor do they bear any obvious similarities in gene content to the SC regions in either Chlamydomonas or Scenedesmus cpDNA.

A strong bias in the distribution of genes between the two DNA strands has been noted in the Stigeoclonium chloroplast genome [20] and, to a lesser extent, in its Scenedesmus counterpart [19]. The propensity of contiguous genes to be encoded on the same strand can be estimated using the sidedness index [30]. Even though several gene clusters are found on the same strand in Oedogonium cpDNA (Fig. 1), the calculated value of 0.74 for the sidedness index indicates that this is the chlorophycean genome where this tendency is the least pronounced (Table 1).

Conserved genes and ORFs

The 99 conserved genes of Oedogonium represent the largest gene repertoire among the four chlorophycean cpDNAs completely sequenced to date (Table 1). A common set of 93 genes, including a fragmented rpoB, are shared by these genomes (Table 2). The collection of conserved genes displayed by Oedogonium closely resembles that of Stigeoclonium, the only differences being the presence of three extra genes [infA, trnR(ccu) and trnR(ucg)] and the absence of trnS(gga) in the former alga (Table 2). Compared to the representatives of the CS clade, both Oedogonium and Stigeoclonium feature three additional genes [psaM, rpl32 and trnL(caa)] and lack petA. These members of the OCC clade also differ from those of the CS clade in exhibiting insertions of more 2,500 codons (2,562 codons in Oedogonium and 2,682 codons in Stigeoclonium) immediately before the 3'conserved region of rps4. The sequences of these insertions are extremely divergent, thereby precluding us from decrypting their nature. Intriguingly, the corresponding 3' regions of the Chlamydomonas and Scenedesmus rps4 genes are missing not only a large insertion but also the highly conserved sequence corresponding to the last 40 codons [15].

Table 2 Differences between the repertoires of conserved genes in Oedogonium and other chlorophycean cpDNAs

As mentioned above, four free-standing ORFs in Oedogonium cpDNA showed no sequence similarity with any genes usually present in chloroplast genomes. Three of these ORFs (orf485, orf512 and orf538) are clustered in the central region of the IR. The contiguous orf485 and orf538 (dpoB in Fig. 1) reside on the same strand as orf512 (int in Fig. 1) but are separated from this ORF by trnR(ccu), a gene located on the opposite strand. The fourth ORF (orf1252) is located within the SC1 region between ftsH and psbK. For all four ORFs, except orf485, our Blast searches identified similarities with genes encoding proteins acting on DNA or RNA.

We found that the orf512 encodes a protein belonging to the family of tyrosine recombinases. The functions of these site-specific recombinases include the integrative and excisive recombination of viral and plasmid DNA into and out of the host genome, conjugative transposition, resolution of catenated DNA circles, regulation of plasmid copy number, and DNA inversions controlling the expression of cell surface proteins or DNA replication [31]. The C-terminal region of the orf512 product displays the essential tyrosine and the five other active site residues that are diagnostic of this protein family [31, 32]. BlastP searches identified the catalytic domain found in the family of phage integrases (pfamH00589) as being the most highly conserved in sequence with the C-terminal region of the orf512-encoded protein. The members of the tyrosine recombinase family that proved the most similar to the Oedogonium orf512 product are the proteins of unknown function encoded by the mitochondrial genomes of two green algae, the charophycean Chaetosphaeridium globosum [25] (E-value threshold of 7 × 10-8) and the trebouxiophycean Prototheca wickerhamii [33] (E-value threshold of 3 × 10-6).

The orf538 encodes a member of the B family of DNA-directed DNA polymerases. These polymerases were named for their homology with the product of the polB gene encoding Escherichia coli polymerase II. Members of this family are extensive in number and variety, occurring in prokaryotes, eukaryotes and viruses [34]. [35]. The DNA polymerase encoded by a linear mitochondrial plasmid in the fungus Neurospora intermedia (the kalilo element; [36]) revealed the highest level of sequence similarity (E-value threshold of 7 × 10-32) with the orf538 product.

BlastP searches with the orf485-encoded protein disclosed limited similarity (E-value threshold of 7 × 10-5) with the conserved domain of the septation ring formation regulator ErzA (pfam06160), a protein that modulates the frequency and position of FtsZ ring formation during the bacterial cell cycle. Because the detected similarity is marginal and restricted to a portion of the ErzA domain, the orf485 product is unlikely to function as an ErzA protein.

Finally, a short C-terminal region of the orf1252 product revealed sequence similarity with the maturase domain of reverse transcriptases encoded by bacterial group II introns and also with the maturase domain of the protein predicted from the free-standing orf932 in Scenedesmus cpDNA [19]. Although they carry the maturase domain, both the Oedogonium orf1252 and Scenedesmus orf932 products lack the reverse transcriptase domain.

Each of the four Oedogonium ORFs was compared with the set of 68 protein-coding genes to assess their similarity in codon usage (Table 3). We found that the orf512/int differs markedly in base composition at the first and third codon positions relative to conserved protein-coding genes; moreover, the codon adaptation index (CAI) [37] calculated for this ORF deviates considerably from the average value obtained for the conserved genes. The orf1252 also differs greatly from conserved protein-coding genes with respect to base composition, but its CAI is not significantly different. For the orf485 and orf538/dpoB, only moderate differences were observed for both the base composition and CAI relative to the conserved protein-coding genes.

Table 3 Compared codon usage of the free-standing ORFs and conserved protein-coding genes in Oedogonium cpDNA

Gene order

Stigeoclonium, Chlamydomonas and Scenedesmus cpDNAs were previously shown to have retained only a few ancestral gene clusters [20]. As illustrated in Fig. 2, the Oedogonium genome has retained about the same set of ancestral clusters. Besides the intact rRNA operon, it displays the psbF-psbL-psbJ cluster and six ancestral gene pairs (atpH-atpF, psbB-psbT, rpl16-rpl14, rpl23-rpl2, rpl5-rps8 and psaJ-rps12). The ancestral gene pairs rpl14-rpl5, rpl2-rps19 as well as the psbH-psbN-psbT cluster are missing from both Oedogonium and Stigeoclonium but are present in both Scenedesmus and Chlamydomonas.

Figure 2

Conservation of gene pairs in Oedogonium and other UTC algal cpDNAs. For each gene pair, adjoining termini of the genes are indicated. Filled boxes indicate the presence of gene pairs with the same relative polarities in two or more genomes. Grey or open boxes indicate the absence of gene pairs. A grey box indicates that the two genes associated with a gene pair are found in the genome but are unlinked. An open box indicates that one or both genes associated with a gene pair are absent from the genome. Two horizontal lines at the bottom of the figure denote the gene pairs that are shared specifically by Chlamydomonas and Scenedesmus (CS clade) and by Oedogonium and Stigeoclonium (OCC clade). The gene pairs shared by the prasinophycean Nephroselmis and the streptophyte Mesostigma viride were presumably present in the last common ancestor of all green algae. Chlorella vulgaris belongs to the Trebouxiophyceae, whereas Oltmannsiellopsis and Pseudendoclonium are representatives of the Ulvophyceae.

With regard to the presence of derived gene clusters, the Oedogonium chloroplast genome clearly bears more similarity with its Stigeoclonium homologue than with the Chlamydomonas and Scenedesmus cpDNAs. The pentad psbT-psbH-psaC(ex1)-psbN-psaC(ex2), the triads atpA-atpI-petG and chlN-psaM-chlB, as well as the gene pairs atpF-rpl16 and trnK(uuu)-trnI(cau) are found in Oedogonium and Stigeoclonium but are absent from the two members of the CS clade (Fig. 2). Of the 11 derived gene clusters previously reported to be shared by Chlamydomonas and Scenedesmus but not by Stigeoclonium, only one (the pair rps7-atpE) was found in Oedogonium, suggesting that it arose before the split of the CS and OCC clades. Note here that the order of the coding regions observed for the fragmented rpoB gene was not considered in Fig. 2. In this regard, it is interesting to point out that the two rpoB gene fragments (rpoBa and rpoBb) are contiguous in the Chlamydomonas and Scenedesmus genomes but separated from one another by other genes in the Oedogonium and Stigeoclonium genomes.

Regardless of their timing of emergence, a total of eight gene clusters are conserved between the Oedogonium and Stigeoclonium cpDNAs; they encode 26 of the 96 genes shared by these genomes. By comparison, 16 conserved gene clusters in the Chlamydomonas and Scenedesmus chloroplasts encode 48 of the 94 genes common to these algal genomes.

Group I Introns

As observed for Stigeoclonium, the Oedogonium chloroplast genome is rich in introns and most of these genetic elements belong to the group I family (Table 1). The 17 group I introns in the Oedogonium chloroplast are distributed among six genes (Table 4). The psbA gene contains ten introns, rrl and psbC each exhibit two introns, and atpA, rrs and trnL(uaa) each contains one intron. These introns vary from 309 bp to 1415 bp in size and, according to the classification system proposed by Michel and Westhof [38], fall within subgroups IA1, IA2, IA3, IB and IC3 (Table 4). Ten introns feature internal ORFs coding for putative homing endonucleases of the HNH, LAGLIDADG and GIY-YIG families (Table 4). All 17 introns, except the rrs intron, are located in the same positions and possess similar structures as compared to introns in other UTC algal chloroplast genomes (Fig. 3). The rrs intron maps to a variable region of the RNA secondary structure and to our knowledge, its insertion site has not been reported previously. The Pseudendoclonium chloroplast genome is the UTC algal genome the most closely related to that of Oedogonium in terms of intron content; these two genomes share a total of 11 introns, including eight in psbA.

Figure 3

Distribution of introns in Oedogonium and other UTC algal cpDNAs. Circles denote the presence of group I introns and squares denote the presence of group II introns. Divided squares represent trans-spliced group II introns. Open symbols denote the absence of intron ORFs, whereas filled symbols denote their presence. Intron insertion sites are designated as indicated in Table 4. The column at the extreme right indicates the introns of Chlamydomonas species other than C. reinhardtii that are known to have homologues in completely sequenced UTC algal genomes. References for the latter introns are as follows: psaB [70]; psbA [71]; psbC [70]; rrs [72]; and rrl [73-76]. An asterisk denotes the absence of the ORF in some Chlamydomonas species.

Table 4 Introns in Oedogonium cpDNA

Group II Introns

The four Oedogonium group II introns range from 736 bp and 2477 bp and occur in psbB, psbI, psaC and petD (Table 4). Both the putatively trans-spliced introns in psaC and petD feature a site of discontinuity within domain I and have positional homologues in Stigeoclonium cpDNA. In contrast, the presence of cis-spliced group II introns in the psbB and psbI gences is documented here for the first time (Fig. 3). The psaC, psbI and petD introns fall within subgroup IIB according to the nomenclature proposed by Michel et al. [39], whereas the psbB intron belongs to subgroup IIA. The predicted secondary structure of the Oedogonium psaC intron bears striking resemblance with that of its Stigeoclonium homologue; however, the Oedogonium petD intron shows a lower degree of similarity with the positionally homologous intron of Stigeoclonium. As shown by the consensus secondary structure of the Oedogonium and Stigeoclonium psaC introns (Fig. 4), the similarity observed for these introns extends well beyond the few residues expected to be conserved in members of the same subclass and their sites of discontinuity are found at the same location within domain I (between D(i)and D(ii)). Only the psbI intron displays an ORF of more than 100 codons; this 458-codon ORF, located within domain IV, specifies a protein carrying a reverse transcriptase domain and a maturase domain.

Figure 4

Consensus secondary structure model of the Oedogonium and Stigeoclonium psaC introns. The model is displayed according to Michel et al. [39]. Exon sequences are shown in lowercase letters. Positions exhibiting different nucleotides are denoted by dots. Positions showing deletions/additions are denoted by arrows labelled with two numbers; the left and right numbers refer to the nucleotides found in the Oedogonium and Stigeoclonium introns, respectively. Conserved base pairings are represented by dashes. Roman numerals specify the six major structural domains, whereas uppercase letters in bold denote the helices in domain I. Upper and lower numbers inside the variable loops indicate the sizes of these loops in the Oedogonium and Stigeoclonium introns, respectively. Tertiary interactions are represented by dashed lines, curved arrows, or Greek lettering. Nucleotides that potentially participate in the δ-δ' interaction are boxed. EBS and IBS are exon-binding and intron-binding sites, respectively. The putative site of lariat formation is denoted by an asterisk.

Repeated sequences

As is the case for the Scenedesmus chloroplast genome, that of Oedogonium contains fewer repeated sequences relative to its Chlamydomonas and Stigeoclonium homologues (Table 1). Repeated sequences of more than 30 bp were estimated to represent 1.28% of the Oedogonium genome. As in other chlorophycean genomes, these repeats map mainly to intergenic regions and most are dispersed repeats. By comparing the sequences of these repeats, we identified two repeat units (A and B). The consensus sequence of repeat unit A (ACACRTWCAATTGTAT) is identical to that of repeat unit B, except that the central region contains CATTG instead of CAATTG. Repeat unit A was identified at 82 genomic sites, whereas repeat unit B was located at 18 locations. Degenerated versions of repeat units A and B were also found in the Oedogonium genome. When our searches allowed a single mismatch at any position in each repeat unit, 293 and 33 copies were recovered for repeat units A and B, respectively. Although the repeats in the Oedogonium genome are generally isolated, some occur in tandem or are closely associated with less frequent repeat units. No repeats having identical sequences to repeat units A and B were detected in any other completely sequenced UTC algal cpDNA.


The distinctive architecture of Oedogonium cpDNA discloses additional characters supporting the split of the Chlorophyceae into two major lineages

The architecture of Oedogonium cpDNA further highlights the extraordinary plasticity of the chlorophycean chloroplast genome. Compared to its Stigeoclonium, Chlamydomonas and Scenedesmus homologues, the 196.6-kb Oedogonium chloroplast genome exhibits high similarity in gene content (Table 2) but differs considerably in gene organization. Of its 99 conserved genes, only two [trnR(ccu) and trnR(ucg)], have not been found in other chlorophycean genomes. Like Chlamydomonas and Scenedesmus cpDNAs, it displays an IR; however, the patterns observed for the partitioning of genes among the two SC regions are distinctly different in these three genomes (this study, [19, 21]). The Oedogonium chloroplast genome resembles its Scenedesmus homologue with reference to its tight packaging of genes and paucity of small dispersed repeats (Table 1). Among the photosynthetic UTC green algae that have been scrutinized thus far for their chloroplast genome [11, 29], this oedogonialean alga shows the highest gene density and lowest proportion of dispersed repeats. Only the 71.7-kb cpDNA of the prasinophycean Ostreococcus tauri [40] and the 37.5-kb plastid DNA of the parasitic trebouxiophycean Helicosporidium sp. [41], two genomes highly reduced in gene content, have been reported to have a more compact gene organization than the Oedogonium genome. In contrast, the Chlamydomonas and Stigeoclonium chloroplast genomes are loosely packed and rich in small dispersed repeats. Oedogonium and Stigeoclonium cpDNAs display a plethora of introns (each containing a total of 21 introns), indicating that the degree of gene packaging has no influence on the size of intron populations observed in chlorophycean genomes. Although members of the group I intron family are predominant in both genomes, introns sharing similar structures and identical insertion positions are found at only five genomic sites (Fig. 3).

Despite its unique architecture, the Oedogonium chloroplast genome revealed shared derived characters (i.e. synapomorphies) at the levels of gene content and gene organization that reinforce our recent study based on comparative analyses of chloroplast gene sequences and gene structural features in supporting the idea that the five recognized lineages of the Chlorophyceae fall within two major clades, the CS and OCC clades [15]. The newly uncovered synapomorphic characters that provide further evidence for the close alliance of the Oedogoniales with the Chaetophorales and for their belonging to a clade separate from the CS clade are as follows: 1) the loss of petA from the chloroplast genome and its putative transfer to the nucleus, 2) the loss of linkage of the two distinct ORFs making up rpoB, 3) the gains of five derived gene clusters (one pentad, two triads and two pairs), and 4) the disruptions of two ancestral gene pairs (rpl14-rpl5 and rpl2-rps19) that are not associated with the formation of the latter derived clusters (Fig. 2). Previously, we showed that the gains of trans-spliced group II introns by petD and psaC and of a large insertion by rps4 distinguish the members of the OCC clade from those belonging to the CS clade [15]. The study reported here also revealed that the disappearance of the psaM, rpl32 and trnL(caa) genes in both Chlamydomonas and Scenedesmus represent additional synapomorphies that provide support for the close alliance of the Chlamydomonadales and Sphaeropleales. Furthermore, we confirmed that the duplication of trnE(cuu) and the acquisitions of all 11 derived clusters reported by de Cambiaire et al. [19], with the exception of rps7-atpE, are synapomorphies uniting the members of the CS clade. Concerning the abovementioned chloroplast genes that underwent losses, it should be pointed out that all four are uniformly present throughout the Chlorophyta, making it extremely unlikely that independent losses (i.e. homoplasy) rather than shared losses were responsible for the distribution patterns observed in the Chlorophyceae. The petA gene was reported to be missing from only Helicosporidium, psaM from Nephroselmis, and trnL(caa) from Oltmannsiellopsis. Given the substantial number of genomic characters that are consistent with the strongly supported conclusions derived from phylogenetic analysis of chloroplast sequence data, we conclude that there is now ample and unambiguous evidence for the split of the Chlorophyceae into two separate clades.

Nevertheless, the precise phylogenetic position of the Oedogoniales within the OCC clade remains uncertain. Phylogenomic analyses of chloroplast sequence data favoured the hypothesis that the Oedogoniales represent the first branch of the OCC clade [15]. Although this topology is supported by the finding that trans-spliced group II introns are present at two identical positions in the rbcL genes of Stigeoclonium and Floydiella but absent from their Oedogonium homologues, the possibility that the Chaetophorales are sister to the Oedogoniales and Chaetopeltidales could not be excluded [15]. To unravel the branching order of the lineages in the OCC clade, we are currently completing the cpDNA sequence of a representative of the Chaetopeltidales (Floydiella) and have undertaken the analysis of additional chlorophyceans to increase taxon sampling in phylogenomic analyses.

The nature of the chloroplast genome in the last common ancestor of all chlorophyceans remains elusive

As observed for the IR-lacking cpDNA of Stigeoclonium [20], the Oedogonium genome proved to be too different from its Chlamydomonas and Scenedesmus counterparts and from the other previously investigated chlorophyte genomes to reconstruct with confidence the landscape of the ancestral chlorophycean genome with regard to the partitioning of genes among the SC regions, the gene order in each of these regions, and the contents in introns and small dispersed repeats. Therefore, it remains unknown whether the ancestral ulvophycean and chlorophycean genomes were similar with respect to their quadripartite structure and whether the transcription direction of the rRNA operon changed before the emergence of both the Chlorophyceae and Ulvophyceae.

The fact that the IR-containing chloroplast genomes from the representatives of the Chlamydomonadales, Sphaeropleales and Oedogoniales feature distinct gene partitioning patterns raises questions as to the factors accounting for this organizational diversity. Currently, there exists no satisfactory general model for chloroplast genome rearrangements. Given that a number of highly rearranged IR-containing cpDNAs display an increased abundance of small dispersed repeats and that the presence of small dispersed repeats and/or tRNA genes has been observed at or near the endpoints of rearranged blocks of genes, it has been proposed that these elements mediate inversions by inter- or intramolecular recombination [12, 25, 26, 4247]. The marked variations in sequence and abundance of small dispersed repeats in the CS and OCC lineages indicate that these repeats evolve in a very dynamic manner; however, whether they played a role in mediating the observed changes in quadripartite structure remains unknown. It is tempting to suggest that dispersed repeats were scarce in the common ancestor of all chlorophyceans and that they arose and proliferated independently in different lineages. Chloroplast genomes of closely and distantly related taxa from separate lineages will need to be investigated to understand the tempo and mode of cpDNA evolution in the Chlorophyceae. At present, only for the Chlamydomonadales do we have chloroplast genome data from more than one representative taxon [48, 49]. These data, which come from physical mapping studies of a large fraction of the genes encoded by the chloroplast genome, suggest that the most phylogenetically divergent chlamydomonadalean taxa investigated (two representatives of the paraphyletic genus Chlamydomonas: Chlamydomonas reinhardtii and Chlamydomonas moewusii) differ by minor changes with respect to their patterns of gene partitioning between the two SC regions [50].

To our surprise, we found that the distribution of group I introns in the Oedogonium genome is strikingly similar to that observed for the ulvophycean Pseudendoclonium (Fig. 3). Whereas 11 of the 17 Oedogonium group I introns, including eight in psbA, are positionally and structurally homologous to Pseudendoclonium introns, only three of the Oedogonium introns have homologues in any of the three other completely sequenced chlorophycean cpDNAs (Fig. 3). Considering that a large fraction of the 27 group I introns found in Pseudendoclonium cpDNA were reported to have no known homologues when they were discovered, that they resembled one another in both secondary structure and primary sequence and that several encoded a putative homing endonuclease, intragenomic proliferation of group I introns was proposed to have occurred within the lineage leading to Pseudendoclonium [12]. The intron mobility conferred by the presence of homing endonuclease genes most likely explains the remarkable similarity between the group I intron contents of the Oedogonium and Pseudendoclonium genomes. Owing to this mobility, we propose that chloroplast group I introns from one chlorophyte taxon were transferred horizontally on multiple occasions to the chloroplast genome of a chlorophyte belonging to a different lineage. Because independent losses of many introns in several lineages would be expected, we consider less likely the scenario postulating that all the introns common to Pseudendoclonium and Oedogonium were present in the last common ancestor of ulvophyceans and chlorophyceans. The ability of mobile group I introns to spread readily to new genomes or new sites within a given genome together with the general property of group I introns to escape from the genomic sites they occupy through reverse splicing [51] are the main explanations for the highly variable distribution patterns observed for the genetic elements belonging to this class in chlorophyte chloroplast genomes (Fig. 3). In contrast, the rare trans-spliced group II introns inserted in chlorophycean genomes are stable, allowing their distribution patterns to be phylogenetically informative [15].

The remarkably large IR of Oedogonium acquired non-standard chloroplast genes via horizontal DNA transfer

The increased size of the Oedogonium IR relative to its Chlamydomonas and Scenedesmus counterparts (Table 1) is largely accounted for by the numerous introns in psbA and by the internal region of about 10 kb that harbours coding sequences not normally found in the chloroplast, notably the int and dpoB genes. The predicted protein encoded by the Oedogonium int gene belongs to the family of tyrosine recombinases, whereas the dpoB product is a member of the B family of DNA-directed DNA polymerases.

Members of the tyrosine recombinase family are most widespread among bacteria and bacteriophages but also occur in archaea and eukaryotes [31, 32]. The integrases of bacteriophage λ and of the conjugative transposons tn916 and tn1545, the E. coli resolvase XerD, and the Cre and Flp recombinases are part of this family. Tyrosine recombinases are responsible for the integration, excision or inversion of defined DNA segments [32]. To mediate site-specific recombination, these proteins recognize an inverted pair of recombination sites (20 to 30 bp in length), break and rejoin single DNA strands in pairs to form a Holliday junction intermediate. Our analyses of the Oedogonium int and of its immediate environment in the IR did not provide enough information to allow a judgment concerning what would be the function of the protein encoded by this gene if it is expressed. Its G+C content and codon usage deviate considerably from what we observed for the conserved protein-coding genes (Table 3), suggesting that low levels of protein, if any, would result from translation of mRNA. Furthermore, no putative recombination sites or features typical of bacterial transposons and integrons could be identified in the 10-kb region housing the unusual genes in the Oedogonium IR.

Genes encoding type B DNA-directed DNA polymerases are commonly found on linear mitochondrial plasmids of yeasts, filamentous fungi and higher plants. These genetic elements encode their own DNA polymerase and RNA polymerase to ensure their autonomous replication [35]. The Oedogonium chloroplast dpoB was found to be closely related to conserved protein-coding genes in terms of G+C content and codon usage (Table 3), implying that it could be actively translated if appropriate regulatory signals allow its transcription by the chloroplast RNA polymerase. The role of such translation product, however, would appear dispensable given that a chloroplast-encoded DNA polymerase dedicated to cpDNA replication is absent from all photosynthetic eukaryotes that have been examined thus far.

Because no cpDNA-encoded homologues of int and dpoB have been reported to date, the origin of these genes in Oedogonium is intriguing. The results of our homology searches in databases point to a mitochondrial origin. The closest homologues of the Oedogonium int gene was identified in the mitochondrial genomes of the green algae Chaetosphaeridium globosum [25] and Prototheca wickerhamii [33], whereas the closest homologue of the Oedogonium dpoB was localized on the kalilo invertron, a linear mitochondrial plasmid of Neurospora intermedia that can integrate into the mitochondrial genome of this fungus [36]. These observations suggest that the Oedogonium int and dpoB genes were acquired through horizontal transfer of mobile element(s) originating from the mitochondria of an unknown donor. As these genes are clustered in the same region of the Oedogonium IR, a single event of horizontal DNA transfer might have been responsible for their insertion in the chloroplast genome.

Although prominent insertions in the IRs of the C. moewusii and Neproselmis chloroplast genomes have also been attributed to horizontal DNA transfer events [23, 52], the nature of the captured sequences remains unknown. The 21-kb insertion in the C. moewusii IR lies between the psbA and rbcL genes [53] and contains five ORFs of more than 200 codons (our unpublished results). In the Nephroselmis IR, a 20.9-kb region between rbcL and the small subunit rRNA gene (rrl) houses 20 ORFs of more than 80 codons, whereas a separate insertion of smaller size (6.9 kb) contains two ORFs [23]. Provided that the Oedogonium, C. moewusii and Nephroselmis insertions were really acquired via lateral transfer, long segments of foreign sequences would have been transferred horizontally to the chloroplast IR on at least four different occasions during the evolution of chlorophytes. As the IR often participates in intramolecular recombination events [54], such events might explain why the IR could to be a preferred target site for the insertion of foreign sequences. On the other hand, the presence of a 5.8-kb insertion carrying ORFs of unknown origin in a SC region of the C. moewusii chloroplast genome [55, 56] suggests that the IR is not the only region that acquired non-standard chloroplast genes through horizontal transfer.

Our study provides the first case of horizontal gene transfer in which coding sequences of known function, not carried out by introns, were gained by the chloroplast genome in the green algal/land plant lineage. Another rare example of horizontal gene transfer involving non-standard chloroplast genes was recently reported for the cryptophyte alga Rhodomonas salina [57]. In this instance, a gene encoding the tau/gamma subunit of DNA polymerase III (dnaX) was most likely acquired from a firmicute bacterium. Only two other convincing cases of horizontal gene transfer in the chloroplast have been documented thus far; both events took place early during the evolution of algae and involved the replacement of native chloroplast genes (the rbcL and rbcS pair and rpl36) by bacterial genes [58, 59].


Our comparative analysis of the Oedogonium chloroplast genome with its homologues in representatives of the Chlamydomonadales, Sphaeropleales and Chaetophorales highlights the extraordinary plasticity of the chlorophycean chloroplast genome and provides compelling evidence for the dichotomy of the Chlorophyceae. No significant insight was gained into the nature of the ancestral chlorophycean chloroplast genome. Unexpectedly, our finding of two non-standard chloroplast genes within the exceptionally large Oedogonium IR has revealed a clear case of horizontal transfer involving most probably the capture of a mobile element from the mitochondria of a fungal or plant species. It will be interesting to see what our ongoing sequencing of Floydiella (Chaetopeltidales) cpDNA will tell us about the branching order of the Oedogoniales, Chaetopeltidales and Chaetophorales and about the forces shaping this genome in the OCC clade.


Cloning and sequencing of Oedogonium cpDNA

Oedogonium cardiacum was obtained from the Sammlung von Algenkulturen Gôttingen (SAG 575-1b) and grown in medium C [60] under 12 h light/dark cycles. An A + T rich fraction containing the cpDNA of this green alga was recovered by centrifugation of total cellular DNA in CsCl-bisbenzimide density gradients [61]. This DNA fraction was sheared by nebulization to produce 1500–2000-bp fragments that were subsequently cloned into the pSMART-HCKan plasmid (Lucigen Corporation, Middleton, WI) [24]. After hybridization of the resulting clones with the original DNA used for cloning, DNA templates from positive clones were prepared with the QIAprep 96 Miniprep kit (Qiagen Inc., Mississauga, Canada) and sequenced as described previously [62]. Sequences were edited and assembled using SEQUENCHER 4.7 (GeneCodes, Ann Arbor, MI). Genomic regions not represented in the clones analyzed were sequenced from PCR-amplified fragments. The fully annotated chloroplast genome sequence has been deposited in [GenBank:EU677193].

Sequence analyses

Genes and ORFs were identified by Blast homology searches [63] against the non-redundant database of the National Center for Biotechnology and Information (NCBI) server. Protein-coding genes and ORFs were localized precisely using ORFFINDER at NCBI, various programs of the Wisconsin package (version 10.3) (Accelrys, San Diego, CA) and applications from the EMBOSS version 4.1.0 package [64]. Positions of genes coding for tRNAs were determined using tRNAscan-SE 1.23 [65]. Boundaries of introns were located by modelling intron secondary structures [38, 39] and by comparing the sequences of intron-containing genes with those of intronless homologues using FRAMEALIGN of the Wisconsin package. For each of the four ORFs identified and for the set of 68 conserved protein-coding genes, both the codon frequency table and the base composition at the three codon positions were calculated with CUSP in EMBOSS. Codon usage bias in conserved genes and ORFs was determined using CAI [37] in EMBOSS. The values derived from the ORFs in these analyses were compared with the corresponding, average value obtained for the conserved protein-coding genes.

Forward and palindromic repeats larger than 20 bp were identified with the Comparative Repeat Analysis program [66]. Number of copies of each repeat unit was determined with FUZZNUC in EMBOSS. Populations of repeats in different chloroplast genomes were compared using the Comparative Repeat Analysis program. Regions of the genome sequence containing non-overlapping repeated elements were mapped with RepeatMasker [67] running under the WU-BLAST 2.0 search engine [68], using the repeats ≥ 30 bp identified with REPuter [69] as input sequences.

Analyses of gene order

The sidedness index Cs or propensity of adjacent genes to occur on the same DNA strand was determined as described by Cui et al. [30] using the formula Cs = (n-nSB)/(n-1), where nSB is the number of sided blocks, i.e. the number of blocks including adjacent genes on the same strand of the genome, and n is the total number of genes. The set of genes used for this analysis did not include the orf485, orf512/int, orf538/dpoB and orf1252. Conserved gene pairs or gene clusters exhibiting identical gene polarities in selected green algal cpDNAs were identified using a custom-built program.


  1. 1.

    Bremer K, Humphries CJ, Mishler BD, Churchill SP: On cladistic relationships in green plants. Taxon. 1987, 36: 339-349.

    Article  Google Scholar 

  2. 2.

    Friedl T: The evolution of the green algae. Pl Syst Evol (Suppl). 1997, 11: 87-101.

    CAS  Article  Google Scholar 

  3. 3.

    Lewis LA, McCourt RM: Green algae and the origin of land plants. Am J Bot. 2004, 91 (10): 1535-1556.

    Article  Google Scholar 

  4. 4.

    O'Kelly CJ, Floyd GL: Flagellar apparatus absolute orientations and the phylogeny of the green algae. BioSystems. 1984, 16 (3–4): 227-251.

    Google Scholar 

  5. 5.

    Pickett-Heaps J: Green Algae: Structure, Reproduction and Evolution in Selected Genera. 1975, Sunderland, Massachusetts: Sinauer Associates, Inc

    Google Scholar 

  6. 6.

    Alberghina JS, Vigna MS, Confalonieri VA: Phylogenetic position of the Oedogoniales within the green algae (Chlorophyta) and the evolution of the absolute orientation of the flagellar apparatus. Pl Syst Evol. 2006, 261: 151-163.

    Article  Google Scholar 

  7. 7.

    Buchheim MA, Michalopulos EA, Buchheim JA: Phylogeny of the Chlorophyceae with special reference to the Sphaeropleales: A study of 18S and 26S rDNA data. J Phycol. 2001, 37 (5): 819-835.

    CAS  Article  Google Scholar 

  8. 8.

    Müller T, Rahmann S, Dandekar T, Wolf M: Accurate and robust phylogeny estimation based on profile distances: a study of the Chlorophyceae (Chlorophyta). BMC Evol Biol. 2004, 4: 20-

    PubMed Central  Article  Google Scholar 

  9. 9.

    Shoup S, Lewis LA: Polyphyletic origin of parallel basal bodies in swimming cells of chlorophycean green algae (Chlorophyta). J Phycol. 2003, 39: 789-796.

    CAS  Article  Google Scholar 

  10. 10.

    Leebens-Mack J, Raubeson LA, Cui L, Kuehl JV, Fourcade MH, Chumley TW, Boore JL, Jansen RK, Depamphilis CW: Identifying the basal angiosperm node in chloroplast genome phylogenies: sampling one's way out of the Felsenstein zone. Mol Biol Evol. 2005, 22 (10): 1948-1963.

    CAS  Article  Google Scholar 

  11. 11.

    Lemieux C, Otis C, Turmel M: A clade uniting the green algae Mesostigma viride and Chlorokybus atmophyticus represents the deepest branch of the Streptophyta in chloroplast genome-based phylogenies. BMC Biology. 2007, 5: 2-

    PubMed Central  Article  Google Scholar 

  12. 12.

    Pombert JF, Otis C, Lemieux C, Turmel M: The chloroplast genome sequence of the green alga Pseudendoclonium akinetum (Ulvophyceae) reveals unusual structural features and new insights into the branching order of chlorophyte lineages. Mol Biol Evol. 2005, 22 (9): 1903-1918.

    CAS  Article  Google Scholar 

  13. 13.

    Qiu Y-L, Li L, Wang B, Chen Z, Knoop V, Groth-Malonek M, Dombrovska O, Lee J, Kent L, Rest J, Estabrook GF, Hendry TA, Taylor DW, Testa CM, Ambros M, Crandall-Stotler B, Duff RJ, Stech M, Frey W, Quandt D, Davis CC: The deepest divergences in land plants inferred from phylogenomic evidence. Proc Natl Acad Sci USA. 2006, 103 (42): 15511-15516.

    CAS  PubMed Central  Article  Google Scholar 

  14. 14.

    Rogers MB, Gilson PR, Su V, McFadden GI, Keeling PJ: The complete chloroplast genome of the chlorarachniophyte Bigelowiella natans: evidence for independent origins of chlorarachniophyte and euglenid secondary endosymbionts. Mol Biol Evol. 2007, 24 (1): 54-62.

    CAS  Article  Google Scholar 

  15. 15.

    Turmel M, Brouard JS, Gagnon C, Otis C, Lemieux C: Deep division in the Chlorophyceae (Chlorophyta) revealed by chloroplast phylogenomic analyses. J Phycol. 2008, 44 (3): 739-750.

    CAS  Article  Google Scholar 

  16. 16.

    Turmel M, Otis C, Lemieux C: The chloroplast genome sequence of Chara vulgaris sheds new light into the closest green algal relatives of land plants. Mol Biol Evol. 2006, 23 (6): 1324-1338.

    CAS  Article  Google Scholar 

  17. 17.

    Jansen RK, Cai Z, Raubeson LA, Daniell H, dePamphilis CW, Leebens-Mack J, Muller KF, Guisinger-Bellian M, Haberle RC, Hansen AK, Chumley TW, Lee S-B, Peery R, McNeal JR, Kuehl JV, Boore JL: Analysis of 81 genes from 64 plastid genomes resolves relationships in angiosperms and identifies genome-scale evolutionary patterns. Proc Natl Acad Sci USA. 2007, 104 (49): 19369-19374.

    CAS  PubMed Central  Article  Google Scholar 

  18. 18.

    Moore MJ, Bell CD, Soltis PS, Soltis DE: Using plastid genome-scale data to resolve enigmatic relationships among basal angiosperms. Proc Natl Acad Sci USA. 2007, 104 (49): 19363-19368.

    PubMed Central  Article  Google Scholar 

  19. 19.

    de Cambiaire JC, Otis C, Lemieux C, Turmel M: The complete chloroplast genome sequence of the chlorophycean green alga Scenedesmus obliquus reveals a compact gene organization and a biased distribution of genes on the two DNA strands. BMC Evol Biol. 2006, 6: 37-

    PubMed Central  Article  Google Scholar 

  20. 20.

    Bélanger AS, Brouard JS, Charlebois P, Otis C, Lemieux C, Turmel M: Distinctive architecture of the chloroplast genome in the chlorophycean green alga Stigeoclonium helveticum. Mol Genet Genomics. 2006, 276 (5): 464-477.

    Article  Google Scholar 

  21. 21.

    Maul JE, Lilly JW, Cui L, dePamphilis CW, Miller W, Harris EH, Stern DB: The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats. Plant Cell. 2002, 14 (11): 2659-2679.

    CAS  PubMed Central  Article  Google Scholar 

  22. 22.

    Pombert JF, Lemieux C, Turmel M: The complete chloroplast DNA sequence of the green alga Oltmannsiellopsis viridis reveals a distinctive quadripartite architecture in the chloroplast genome of early diverging ulvophytes. BMC Biology. 2006, 4: 3-

    PubMed Central  Article  Google Scholar 

  23. 23.

    Turmel M, Otis C, Lemieux C: The complete chloroplast DNA sequence of the green alga Nephroselmis olivacea: insights into the architecture of ancestral chloroplast genomes. Proc Natl Acad Sci USA. 1999, 96: 10248-10253.

    CAS  PubMed Central  Article  Google Scholar 

  24. 24.

    Lemieux C, Otis C, Turmel M: Ancestral chloroplast genome in Mesostigma viride reveals an early branch of green plant evolution. Nature. 2000, 403 (6770): 649-652.

    CAS  Article  Google Scholar 

  25. 25.

    Turmel M, Otis C, Lemieux C: The chloroplast and mitochondrial genome sequences of the charophyte Chaetosphaeridium globosum: insights into the timing of the events that restructured organelle DNAs within the green algal lineage that led to land plants. Proc Natl Acad Sci USA. 2002, 99 (17): 11275-11280.

    CAS  PubMed Central  Article  Google Scholar 

  26. 26.

    Palmer JD: Plastid chromosomes: structure and evolution. The Molecular Biology of Plastids. Edited by: Bogorad L, Vasil K. 1991, San Diego: Academic Press, 5-53.

    Chapter  Google Scholar 

  27. 27.

    Raubeson LA, Jansen RK: Chloroplast genomes of plants. Diversity and Evolution of Plants: Genotypic and Phenotypic Variation in Higher Plants. Edited by: Henry RJ. 2005, Wallingford: CABI Publishing, 45-68.

    Chapter  Google Scholar 

  28. 28.

    Turmel M, Pombert JF, Charlebois P, Otis C, Lemieux C: The green algal ancestry of land plants as revealed by the chloroplast genome. Int J Plant Sci. 2007, 168 (5): 679-689.

    CAS  Article  Google Scholar 

  29. 29.

    de Cambiaire JC, Otis C, Lemieux C, Turmel M: The chloroplast genome sequence of the green alga Leptosira terrestris: multiple losses of the inverted repeat and extensive genome rearrangements within the Trebouxiophyceae. BMC Genomics. 2007, 8: 213-

    PubMed Central  Article  Google Scholar 

  30. 30.

    Cui L, Leebens-Mack J, Wang LS, Tang J, Rymarquis L, Stern DB, dePamphilis CW: Adaptive evolution of chloroplast genome structure inferred using a parametric bootstrap approach. BMC Evol Biol. 2006, 6: 13-

    PubMed Central  Article  Google Scholar 

  31. 31.

    Nunes-Duby SE, Kwon HJ, Tirumalai RS, Ellenberger T, Landy A: Similarities and differences among 105 members of the Int family of site-specific recombinases. Nucl Acids Res. 1998, 26 (2): 391-406.

    CAS  PubMed Central  Article  Google Scholar 

  32. 32.

    Grindley NDF, Whiteson KL, Rice PA: Mechanisms of site-specific recombination. Annu Rev Biochem. 2006, 75 (1): 567-605.

    CAS  Article  Google Scholar 

  33. 33.

    Wolff G, Plante I, Lang BF, Kück U, Burger G: Complete sequence of the mitochondrial DNA of the chlorophyte alga Prototheca wickerhamii. J Mol Biol. 1994, 237: 75-86.

    CAS  Article  Google Scholar 

  34. 34.

    Ito J, Braithwaite DK: Compilation and alignment of DNA polymerase sequences. Nucl Acids Res. 1991, 19 (15): 4045-4057.

    CAS  PubMed Central  Article  Google Scholar 

  35. 35.

    Kempken F, Hermanns J, Osiewacz HD: Evolution of linear plasmids. J Mol Evol. 1992, 35: 502-513.

    CAS  Article  Google Scholar 

  36. 36.

    Chan BSS, Court DA, Vierula J, Bertrand H: The kalilo linear senescence-inducing plasmid of Neurospora is an invertron and encodes DNA and RNA polymerases. Curr Genet. 1991, 20: 225-237.

    CAS  Article  Google Scholar 

  37. 37.

    Sharp PM, Li W-H: The codon adaptation index – a measure of directional synonymous codon usage bias, and its potential applications. Nucl Acids Res. 1987, 15 (3): 1281-1295.

    CAS  PubMed Central  Article  Google Scholar 

  38. 38.

    Michel F, Westhof E: Modelling of the three-dimensional architecture of group I catalytic introns based on comparative sequence analysis. J Mol Biol. 1990, 216 (3): 585-610.

    CAS  Article  Google Scholar 

  39. 39.

    Michel F, Umesono K, Ozeki H: Comparative and functional anatomy of group II catalytic introns – a review. Gene. 1989, 82 (1): 5-30.

    CAS  Article  Google Scholar 

  40. 40.

    Robbens S, Derelle E, Ferraz C, Wuyts J, Moreau H, Peer Van de Y: The complete chloroplast and mitochondrial DNA sequence of Ostreococcus tauri: organelle genomes of the smallest eukaryote are examples of compaction. Mol Biol Evol. 2007, 24 (4): 956-968.

    CAS  Article  Google Scholar 

  41. 41.

    de Koning AP, Keeling PJ: The complete plastid genome sequence of the parasitic green alga Helicosporidium sp. is highly reduced and structured. BMC Biology. 2006, 4: 12-

    PubMed Central  Article  Google Scholar 

  42. 42.

    Chumley TW, Palmer JD, Mower JP, Fourcade HM, Calie PJ, Boore JL, Jansen RK: The complete chloroplast genome sequence of Pelargonium × hortorum: organization and evolution of the largest and most highly rearranged chloroplast genome of land plants. Mol Biol Evol. 2006, 23 (11): 2175-2190.

    CAS  Article  Google Scholar 

  43. 43.

    Haberle RC, Fourcade HM, Boore JL, Jansen RK: Extensive rearrangements in the chloroplast genome of Trachelium caeruleum are associated with repeats and tRNA genes. J Mol Evol. 2008, 66 (4): 350-361.

    CAS  Article  Google Scholar 

  44. 44.

    Hiratsuka J, Shimada H, Whittier R, Hishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun C-R, Meng B-Y, Li Y-Q, Kanno A, Nishizawa Y, Hirai A, Shinozaki K, Sugiura M: The complete sequence of the rice (Oryza sativa) chloroplast genome: Intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals. Mol Gen Genet. 1989, 217: 185-194.

    CAS  Article  Google Scholar 

  45. 45.

    Howe CJ, Barker RF, Bowman CM, Dyer TA: Common features of three inversions in wheat chloroplast DNA. Curr Genet. 1988, 13 (4): 343-350.

    CAS  Article  Google Scholar 

  46. 46.

    Milligan BG, Hampton JN, Palmer JD: Dispersed repeats and structural reorganization in subclover chloroplast DNA. Mol Biol Evol. 1989, 6 (4): 355-368.

    CAS  Google Scholar 

  47. 47.

    Tsai CH, Strauss SH: Dispersed repetitive sequences in the chloroplast genome of douglas-fir. Curr Genet. 1989, 16 (3): 211-218.

    CAS  Article  Google Scholar 

  48. 48.

    Boudreau E, Turmel M: Gene rearrangements in Chlamydomonas chloroplast DNAs are accounted for by inversions and by the expansion/contraction of the inverted repeat. Plant Mol Biol. 1995, 27 (2): 351-364.

    CAS  Article  Google Scholar 

  49. 49.

    Boudreau E, Turmel M: Extensive gene rearrangements in the chloroplast DNAs of Chlamydomonas species featuring multiple dispersed repeats. Mol Biol Evol. 1996, 13 (1): 233-243.

    CAS  Article  Google Scholar 

  50. 50.

    Boudreau E, Otis C, Turmel M: Conserved gene clusters in the highly rearranged chloroplast genomes of Chlamydomonas moewusii and Chlamydomonas reinhardtii. Plant Mol Biol. 1994, 24 (4): 585-602.

    CAS  Article  Google Scholar 

  51. 51.

    Dujon B: Group I introns as mobile genetic elements: Facts and mechanistic speculations – A review. Gene. 1989, 82: 91-114.

    CAS  Article  Google Scholar 

  52. 52.

    Lemieux B, Turmel M, Lemieux C: Unidirectional gene conversions in the chloroplast of Chlamydomonas interspecific hybrids. Mol Gen Genet. 1988, 212: 48-55.

    CAS  Article  Google Scholar 

  53. 53.

    Lemieux C, Turmel M, Lee RW, Bellemare G: A 21 kilobase-pair deletion/addition difference in the inverted repeat sequence of chloroplast DNA from Chlamydomonas eugametos and C. moewusii. Plant Mol Biol. 1985, 5: 77-84.

    CAS  Article  Google Scholar 

  54. 54.

    Palmer JD: Chloroplast DNA exists in two orientations. Nature. 1983, 301: 92-93.

    CAS  Article  Google Scholar 

  55. 55.

    Bergeron E: Analyse structurale d'un ADN linéaire de six kilopaires de bases chez Chlamydomonas moewusii. 1990, Québec: Université Laval

    Google Scholar 

  56. 56.

    Turmel M, Bellemare G, Lee RW, Lemieux C: A linear DNA molecule of 5.9 kilobase-pairs is highly homologous to the chloroplast DNA in the green alga Chlamydomonas moewusii. Plant Mol Biol. 1986, 313-319. 6

  57. 57.

    Khan H, Parks N, Kozera C, Curtis BA, Parsons BJ, Bowman S, Archibald JM: Plastid genome sequence of the cryptophyte alga Rhodomonas salina CCMP1319: lateral transfer of putative DNA replication machinery and a test of chromist plastid phylogeny. Mol Biol Evol. 2007, 24 (8): 1832-1842.

    CAS  Article  Google Scholar 

  58. 58.

    Delwiche CF, Palmer JD: Rampant horizontal transfer and duplication of rubisco genes in eubacteria and plastids. Mol Biol Evol. 1996, 13 (6): 873-882.

    CAS  Article  Google Scholar 

  59. 59.

    Rice DW, Palmer JD: An exceptional horizontal gene transfer in plastids: gene replacement by a distant bacterial paralog and evidence that haptophyte and cryptophyte plastids are sisters. BMC Biol. 2006, 4: 31-

    PubMed Central  Article  Google Scholar 

  60. 60.

    Andersen RA, Berges JA, Harrison PJ, Watanabe MM: Appendix A – Recipes for freswater and seawater media. Algal Culturing Techniques. Edited by: Andersen RA. 2005, Burlington: Elsevier Academic Press, 429-538.

    Google Scholar 

  61. 61.

    Turmel M, Lemieux C, Burger G, Lang BF, Otis C, Plante I, Gray MW: The complete mitochondrial DNA sequences of Nephroselmis olivacea and Pedinomonas minor: two radically different evolutionary patterns within green algae. Plant Cell. 1999, 11 (9): 1717-1729.

    CAS  PubMed Central  Google Scholar 

  62. 62.

    Turmel M, Otis C, Lemieux C: The mitochondrial genome of Chara vulgaris: insights into the mitochondrial DNA architecture of the last common ancestor of green algae and land plants. Plant Cell. 2003, 15: 1888-1903.

    CAS  PubMed Central  Article  Google Scholar 

  63. 63.

    Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215: 403-410.

    CAS  Article  Google Scholar 

  64. 64.

    Rice P, Longden I, Bleasby A: EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 2000, 16 (6): 276-277.

    CAS  Article  Google Scholar 

  65. 65.

    Lowe TM, Eddy SR: tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 1997, 25: 955-964.

    CAS  PubMed Central  Article  Google Scholar 

  66. 66.

    Comparative Repeat Analysis. []

  67. 67.

    RepeatMasker. []

  68. 68.

    WU-BLAST. []

  69. 69.

    Kurtz S, Choudhuri JV, Ohlebusch E, Schleiermacher C, Stoye J, Giegerich R: REPuter: the manifold applications of repeat analysis on a genomic scale. Nucleic Acids Res. 2001, 29 (22): 4633-4642.

    CAS  PubMed Central  Article  Google Scholar 

  70. 70.

    Turmel M, Mercier J-P, Côté M-J: Group I introns interrupt the chloroplast psaB and psbC and the mitochondrial rrnL gene in Chlamydomonas. Nucleic Acids Res. 1993, 21: 5242-5250.

    CAS  PubMed Central  Article  Google Scholar 

  71. 71.

    Turmel M, Boulanger J, Lemieux C: Two group I introns with long internal open reading frames in the chloroplast psbA gene of Chlamydomonas moewusii. Nucleic Acids Res. 1989, 17 (10): 3875-3887.

    CAS  PubMed Central  Article  Google Scholar 

  72. 72.

    Durocher V, Gauthier A, Bellemare G, Lemieux C: An optional group I intron between the chloroplast small subunit rRNA genes of Chlamydomonas moewusii and C. eugametos. Curr Genet. 1989, 15 (4): 277-282.

    CAS  Article  Google Scholar 

  73. 73.

    Côté V, Mercier J-P, Lemieux C, Turmel M: The single group-I intron in the chloroplast rrnL gene of Chlamydomonas humicola encodes a site-specific DNA endonuclease (I-Chu I). Gene. 1993, 129 (1): 69-76.

    Article  Google Scholar 

  74. 74.

    Turmel M, Boulanger J, Schnare MN, Gray MW, Lemieux C: Six group I introns and three internal transcribed spacers in the chloroplast large subunit ribosomal RNA gene of the green alga Chlamydomonas eugametos. J Mol Biol. 1991, 218 (2): 293-311.

    CAS  Article  Google Scholar 

  75. 75.

    Turmel M, Côté V, Otis C, Mercier J-P, Gray MW, Lonergan KM, Lemieux C: Evolutionary transfer of ORF-containing group I introns between different subcellular compartments (chloroplast and mitochondrion). Mol Biol Evol. 1995, 12 (4): 533-545.

    CAS  Google Scholar 

  76. 76.

    Turmel M, Gutell RR, Mercier J-P, Otis C, Lemieux C: Analysis of the chloroplast large subunit ribosomal RNA gene from 17 Chlamydomonas taxa. Three internal transcribed spacers and 12 group I intron insertion sites. J Mol Biol. 1993, 232 (2): 446-467.

    CAS  Article  Google Scholar 

Download references


We thank Harold Anglehart for his assistance in determining the Oedogonium chloroplast genome sequence. This work was supported by the Natural Sciences and Engineering Research Council of Canada (to CL and MT).

Author information



Corresponding author

Correspondence to Monique Turmel.

Additional information

Authors' contributions

J–SB participated in the conception of this study, performed most of the sequence analyses, generated the tables and figures and drafted the manuscript. CO performed the sequencing and contributed to the assembly and annotation of the genome sequence. CL and MT conceived and supervised the study, contributed to the analysis and interpretation of the data and prepared the manuscript. All authors read and approved the final manuscript.

Authors’ original submitted files for images

Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Brouard, JS., Otis, C., Lemieux, C. et al. Chloroplast DNA sequence of the green alga Oedogonium cardiacum (Chlorophyceae): Unique genome architecture, derived characters shared with the Chaetophorales and novel genes acquired through horizontal transfer. BMC Genomics 9, 290 (2008).

Download citation


  • Inverted Repeat
  • Chloroplast Genome
  • rRNA Operon
  • Codon Adaptation Index
  • Small Single Copy