Skip to main content

Table 2 Expression changes accompanying environmental challenge in T. brucei.

From: The trypanosome transcriptome is remodelled during differentiation but displays limited responsiveness within life stages

    Fold up (down) vs control
Experiment Gene Accession array qRT-PCR
SMB grown with 0%FBS 5 mg/ml BSA vs with 10% FBS Gp63 (TbMSP-C) Tb10.70.5290 2.9 2.2
  procyclin Tb10.6k15.0030 2.4 2.4
  procyclin Tb927.6.450 2.0 2.3
  cysteine peptidase Tb927.6.960 1.9 3.6
  ESAG6 H25N7.15 1.8 3.9
  ESAG7 N19B2.155 1.6 4.7
  14-3-3-like Tb11.02.4700 (1.9)  
SMB grown with 0%FBS, 5 mg/ml BSA, 0.3 mg/mg Tfn vs with 10% FBS Gp63 (TbMSP-C) Tb10.70.5290 3.1 1.5
  procyclin Tb10.6k15.0030 2.2 1.9
  cysteine peptidase Tb927.6.960 2.0 4.6
  ESAG6 H25N7.15 2.0 4.1
  ESAG7 N19B2.155 1.4 3.5
SMB grown with 30%FBS vs with 10% FBS ISG65-like Tb927.5.410 (2.9) (4.1)
  CAMK4 kinase Tb927.4.3770 (2.2)  
  procyclin Tb10.6k15.0030 (1.9) (3.6)
SMB grown for 1 hr with 1 mM DTT vs without GTP1/OBG/Hsr1 Tb927.2.5060 2.4  
  putative ABC transporter Tb10.329.0040 2.2  
  ISG65-like Tb927.5.410 (2.9) (1.9)
  ESAG7 N19B2.155 (2.2) (2.0)
  MAP kinase Tb10.70.2070 (2.1)  
  Rab3GAP Tb10.6k15.1390 (2.1)  
  dual-specificity protein phosphatase Tb927.5.3620 (1.9)  
SMB grown for 4 hr with 1 mM DTT vs without TbGRP Tb10.6k15.1520 3.6  
  hypothetical ER chaperone Tb11.01.8120 3.0 3.3
  Ubiquitin conjugating Tb927.7.6960 2.8  
  Vta1-like Tb927.7.7140 2.7  
  syntaxin Tb10.61.1980 2.7  
  Hsp60 chaperonin Tb10.70.0280 (3.0)  
  GMP-PDE delta Tb927.2.4580 (2.8)  
  Rab11a Tb927.8.4330 (2.8) (1.8)
  Ser peptidase (Bem46-like) Tb09.211.2310 (2.4)  
  Sec24 Tb927.3.5420 (2.4)  
SMB grown for 4 hr with 5 ug/ml Tunicamycin vs without procyclin Tb10.6k15.0030 2.4 2.1
  ESAG6 H25N7.15 1.9 2.3
  procyclin Tb927.6.450 1.7 2.3
  AP2-kinase1 Tb09.160.4770 (2.0)  
SMB grown for 24 hr with 5 ug/ml Tunicamycin vs without procyclin Tb10.6k15.0030 4.2 7.8
  procyclin Tb927.6.450 2.7 8.8
  ESAG7 N19B2.155 2.6 4.3
  ESAG6 H25N7.15 2.2 2.8
  RabGAP Tb10.6k15.1790 2.1  
  GOLD domain Tb927.7.3600 2.1  
  PDI/ERp72 Tb10.6k15.2290 (2.3)  
VSG RNAi induced vs uninduced Day1 RabGEF/Vps9 Tb927.3.2430 2.1  
VSG RNAi induced vs uninduced Day3 nothing    
CLH RNAi induced vs uninduced 24 hr Gp63 (TbMSP-C) Tb10.70.5290 2.7 3.6
  ISG65-like Tb927.5.410 2.8 3.8
  CLH Tb10.70.0830 (2.5)  
  GMP-PDE delta Tb927.2.4580 (2.3)  
  CBL-interacting protein kinase 9 Tb927.8.870 (2.0)  
Rab5AWT overexpressing PCF line vs PCF WT (both with 20% FBS) alpha tubulin Tb927.1.2400 2.9 1.7
  histone H3 Tb927.1.2430 2.7 1.5
  Rab5a Tb10.389.0550 2.6 9.5
  RabX3 Tb11.01.6670 2.5 3.5
  40S ribosomal protein S25 Tb927.3.1370 2.2 1.2
  Amphiphysin Tb11.01.6740 2.2  
  Hsp70 Tb927.6.3740 2.0  
  cysteine peptidase Tb927.6.960 2.0  
  COPI epsilon Tb11.01.6530 2.0 2.6
  adenylate kinase 3 Tb10.70.5150 2.0 3.1
  CBL-interacting protein kinase 9 Tb927.8.870 (2.3) 1.6
  Ubiquitin conjugating Tb11.02.0815 (2.1) 1.6
  ARPC5 Tb10.406.0320 (2.0)  
Rab5AQL overexpressing PCF line vs PCF WT histone H3 Tb927.1.2430 2.3 1.6
  40S ribosomal protein S25 Tb927.3.1370 2.1 1.1
  Rab5a Tb10.389.0550 2.0 9.8
  adenylate kinase 3 Tb10.70.5150 1.6 3.8
  alpha tubulin Tb927.1.2400 1.6 2.1
  COPI epsilon Tb11.01.6530 1.4 3.0
  RabX3 Tb11.01.6670 1.3 3.4
  mitochondrial intermediate peptidase Tb10.6k15.0490 (2.4)  
  mitochondrial zinc metallopeptidase Tb927.4.3300 (2.0)  
Rab5BQL overexpressing PCF line vs PCF WT Rab5b Tb11.02.2160 2.5 16.7
  adenylate kinase 3 Tb10.70.5150 1.6 3.5
  COPI epsilon Tb11.01.6530 1.5 3.4
  RabX3 Tb11.01.6670 1.5 2.8
  1. The most significantly altered mRNAs detected by microarray for differing serum conditions, dithiothreitol and tunicamycin treatment, RNAi of VSG or CLH, and Rab5 overexpressing PCF lines are shown. The ratios obtained by qRT-PCR for selected transcripts are also shown. Ratios are given on a linear (not log) scale, and those indicating a reduction compared to the control state are in parentheses. Only ORFs that display at least a two-fold change, by either qRT-PCR or array, are included; except the 4 hour DTT treatment, where only the top ten hits are shown. Ratios which correspond to a value >1 for the B-statistic in limma analysis are shown in bold.
\