Figure 1
From: Assessing the feasibility of GS FLX Pyrosequencing for sequencing the Atlantic salmon genome
Nine BACs within the minimum tiling path (MTP) of Atlantic salmon contig 483. Using the BAC-end sequences, primers were developed to amplify sequence tag sites (STSs – vertical lines), which were used to design and verify a minimum tiling path across the contig. BAC S0022P24 (green line) was sequenced using traditional Sanger sequencing of a shotgun library and the remaining eight BACs (black lines) were sequenced using the GS FLX platform.