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Figure 5 | BMC Genomics

Figure 5

From: A combination of LongSAGE with Solexa sequencing is well suited to explore the depth and the complexity of transcriptome

Figure 5

Effect of the library size on the number of unique tags identified. The three figures represent the number of unique tags identified as a function of the total number of tags in random libraries. These libraries were obtained by random sampling of X tags in the library considered (Sanger_Hypo or Solexa_Hypo), where X vary from 1 to the total number of tags in this library. In each of the obtained samples, we also calculated the number of unique tags that matches to the mouse genome (dotted lines). We considered that a tag matches to the genome when it has 100% identity over its whole length (21 bp). A: Figure for the Sanger_Hypo library. B: Figure for the Solexa_Hypo library. C: Figure comparing the number of unique tags identified as a function of the total number of tags between the Sanger_Hypo and the Solexa_Hypo library. The size of the random samples varies consequently from 1 to the size of the Sanger_Hypo library (the smallest of the two libraries).

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