- Methodology article
- Open Access
A combination of LongSAGE with Solexa sequencing is well suited to explore the depth and the complexity of transcriptome
- Lucie Hanriot†1,
- Céline Keime†2,
- Nadine Gay1,
- Claudine Faure3,
- Carole Dossat4, 5, 6,
- Patrick Wincker4, 5, 6,
- Céline Scoté-Blachon1,
- Christelle Peyron1 and
- Olivier Gandrillon3Email author
© Hanriot et al; licensee BioMed Central Ltd. 2008
- Received: 21 April 2008
- Accepted: 16 September 2008
- Published: 16 September 2008
"Open" transcriptome analysis methods allow to study gene expression without a priori knowledge of the transcript sequences. As of now, SAGE (Serial Analysis of Gene Expression), LongSAGE and MPSS (Massively Parallel Signature Sequencing) are the mostly used methods for "open" transcriptome analysis. Both LongSAGE and MPSS rely on the isolation of 21 pb tag sequences from each transcript. In contrast to LongSAGE, the high throughput sequencing method used in MPSS enables the rapid sequencing of very large libraries containing several millions of tags, allowing deep transcriptome analysis. However, a bias in the complexity of the transcriptome representation obtained by MPSS was recently uncovered.
In order to make a deep analysis of mouse hypothalamus transcriptome avoiding the limitation introduced by MPSS, we combined LongSAGE with the Solexa sequencing technology and obtained a library of more than 11 millions of tags. We then compared it to a LongSAGE library of mouse hypothalamus sequenced with the Sanger method.
We found that Solexa sequencing technology combined with LongSAGE is perfectly suited for deep transcriptome analysis. In contrast to MPSS, it gives a complex representation of transcriptome as reliable as a LongSAGE library sequenced by the Sanger method.
- Massively Parallel Signature Sequencing
- Solexa Sequencing
- Sanger Method
- Allen Brain Atlas
- Massively Parallel Signature Sequencing Data
Methods for transcriptome analysis are today diversified and can be divided in two families of technologies: "closed" and "open" techniques . In closed technologies such as microarrays, the space of inquiry is finite since the analysis of the expression level is limited to previously characterized transcript sequences for which a corresponding probe was spotted on the microarray. In contrary, open technologies analyze the transcriptome without any a priori knowledge on the transcript sequences. These methods thus allow the discovery of new transcribed sequences [2–5]. This is particularly interesting as all transcribed sequences have not been discovered yet, even in well studied species like mouse  and human .
The most widely used methods for open transcriptome analysis are based on the sequencing of either cDNAs (known as Expressed Sequence Tags or ESTs) or of short tag sequences. This later strategy has been developed in Serial Analysis of Gene Expression (SAGE) , LongSAGE  and Massively Parallel Signature Sequencing (MPSS) . They are by construction much more efficient in sampling the depth of the transcriptome than the EST sequencing techniques. In contrast to the 14 bp SAGE tags generated by SAGE, the 21 bp tags obtained either by LongSAGE or MPSS can directly be mapped to the genome sequence, which is particularly interesting for the identification of new transcribed sequences .
Today, the new challenge of gene expression analysis is the deep analysis of transcriptomes in order to investigate the role of weakly expressed genes that can nevertheless play an important role in different biological processes. A recent study revealed that millions of transcript tags have to be sequenced in order to fully characterize a human transcriptome ([8–10]). In this respect, the MPSS technique is particularly interesting as its bead-based sequencing technology allows to sequence simultaneously more than one million of tags in a library. This is therefore far more efficient and faster than the sequencing of a LongSAGE library by the Sanger method. However, recent publication shows that MPSS libraries are significantly less complex than much smaller LongSAGE libraries, revealing a serious bias in the generation of MPSS data ([11, 12]).
We therefore built up a library of male adult mice hypothalamus, a brain region involved in behavioral and autonomic coordination, using LongSAGE and Solexa sequencing technologies. In order to assess if we can properly explore the transcriptome complexity with this method, we compared this library with a LongSAGE library of mouse hypothalamus sequenced with the Sanger method. We found that, for the same number of tags, a comparable and even slightly higher level of complexity of transcriptome is uncovered with LongSAGE combined with the Solexa technology than with the Sanger method. Therefore, the combination of LongSAGE and Solexa sequencing seems to be perfectly suited for deep transcriptome analysis.
Major characteristics of the two libraries
Major characteristics of the Sanger_Hypo and Solexa_Hypo LongSAGE libraries
Total number of tags
Number of unique tags
Number of tags with only one occurrence
% of tags matching to the genome*
The Solexa_Hypo library is 162 fold deeper (11,017,712 tags) than the Sanger_Hypo library (68,023 tags). Far more unique tags were therefore obtained in the Solexa_Hypo library (609,407) than in the Sanger_Hypo one (23,007) (Table 1). With both the Sanger and the Solexa techniques, a very high percent of the sequenced tags matched to the mouse genome (90.28 and 92.25% of the Sanger_Hypo and Solexa_Hypo tags respectively, Table 1). This proportion is higher than a previous estimation (82.7%) computed by estimating the proportion of erroneous tags in LongSAGE libraries . This could be due to our consideration of the base-call quality during the extraction of tags from concatemer sequences (see Method section). Nevertheless, it is to keep in mind that the proportion of tags that do not match to the genome is an over-estimation of the proportion of the erroneous tags. Indeed, several tags without errors do not match to the genome because they overlap two exons, extend into the polyA tail or contain polymorphic positions . Furthermore, the probability that an erroneous tag match to the genome has been estimated to be very low . Consequently, the tag sequences obtained in both Solexa_Hypo and Sanger_Hypo libraries seem to be of excellent quality, and the overall quality of the Solexa_Hypo tags seems to be slightly better than the Sanger_Hypo ones.
One could argue that the better score obtained with Solexa_Hypo is due to the fact that this library is created from C57BL/6 mice, the same mice strain that has been used to sequence the mouse genome while the Sanger_Hypo library come from fvb mice. It is probably partly valid. However, Sandberg et al  have estimated at 1% the number of tags that are different between 2 mouse strains. Furthermore, we estimated the percent of tags that match with one mismatch on the C57BL6/J genome to be of 4.05% and 2.29% for the Sanger_Hypo and the Solexa_Hypo libraries respectively. This is a very low percentage, thereby confirming the very low probability that an erroneous tag matches to the genome. This percentage is only slightly higher for Sanger_Hypo indicating that the better score obtained with Solexa_Hypo is not only due to strain differences.
When we consider all unique tags from the two libraries combined, 2.8% of these tags are found in both libraries, 96.3% are found only in the Solexa_Hypo library, and 0.9% are found only in the Sanger_Hypo library. As one would expect, the mean occurrence number of the 2.8% common tags is higher (51.4 tpm in Sanger_Hypo and 34.2 tpm in Solexa_Hypo library) than the mean occurrence number of all tags (43.5 tpm in Sanger_Hypo and 1.6 tpm in Solexa_Hypo) in both libraries. Furthermore, if we select the 100 most abundant unique tags from both libraries (See Additional file 1), the number of unique tags found in both libraries is greatly increased (46%). These data confirm that the depth of sequencing of the Sanger_Hypo library is not sufficient to sample the tags present in the initial hypothalamic sample. The 0.9% of tags found uniquely in the Sanger_Hypo library might be mostly due to mouse strain differences (this is concordant with the estimation of 1% of transcriptomic differences between mouse strains by Sandberg et al. ).
Depth of sampling
Theoretically, this rate should equal zero if all unique tags of the initial hypothalamus sample had been sampled. However, it could be slightly greater because the addition of new tags could be due to the accumulation of different sequencing errors as the size of the library increases. We thus calculated the number of unique tags that matches to the genome in each of the random sampled libraries (dotted lines in Figure 5). As the probability that an erroneous tag matches to the genome is very low , this set of matching tags should contains almost only reliable tags. There is a smaller increase of the number of unique tags identified as the size of the library increases when we only consider matching tags than when we consider all tags (compare lines and dotted lines in the Figure 5). Moreover, the dotted line in the Figure 3A confirms that the size of the Sanger_Hypo library does not sample all unique tags from the initial hypothalamic sample. In contrary, it seems that we have almost sampled all different tags in the Solexa_Hypo library since the rate of discovery of new unique tags is severely dropping as the size increases: with a library size of one million, we were able to identify only one third of the unique tags (about 1.4 × 105 unique tags detected out of the total of 3.9 × 105 observed for the full library) whereas 80% of these tags were identified with a library of 5 millions of tags, and the vast majority (98%) in a 10 millions tags library (Figure 5B)."
Complexity of the transcriptome
Increasing sequencing depth is only valuable if it gives access to a better image of the transcriptome complexity (i.e. the unique tags in the analyzed sample). We therefore compared the transcriptome complexity for the same random library sizes in the Sanger_Hypo and in the Solexa_Hypo SAGE libraries (Figure 5C) and found that LongSAGE combined either with Sanger or with Solexa sequencing gives access to similar transcriptome complexity. The number of unique tags identified in the Solexa_Hypo library is even slightly larger than the one in the Sanger_Hypo library (see the dotted lines on Figure 5C).
Hene et al.  showed that at a same sampling depth, a LongSAGE library of human T cells sequenced with the Sanger method contains much more unique tags than a MPSS library of T-cells (71,838 and 9,723 unique tags matching to the human genome for a sample size of 500,000 tags respectively). When we consider the same library size as Hene and colleagues, we sampled 118,075 unique tags matching to the mouse genome from the Solexa_Hypo library. Although a significant part of this difference may be accounted to the higher complexity of the hypothalamic sample used for the Sanger_Hypo and Solexa_Hypo libraries compared to the T cell sample used by Hene et al. , it does not explain it all. It rather advocates that LongSAGE – Solexa does not have the limitation of MPSS in its ability to explore the transcriptome complexity. Indeed, at a same sampling depth of 60,000 tags, considering only tags that match the genome, we found 21,133 unique tags in the Sanger_Hypo library and 25,604 unique tags in the Solexa_Hypo library (20% more), suggesting an ability of LongSAGE – Solexa to uncover more transcriptome complexity than LongSAGE – Sanger method.
The present study shows that the Solexa sequencing technology is well adapted to the sequencing of LongSAGE tags, allowing to rapidly obtain a very deep LongSAGE library without the complexity limitation observed in MPSS libraries. The combination of LongSAGE and Solexa sequencing technology is therefore perfectly suited for deep transcriptome analysis.
Adult male mice (12–14 weeks of age), kept on a 12:12 light-dark cycle (light on at 7 am), were sacrificed by decapitation following the ethical committee's instructions (BH-2006-06) between 10 and 11 am. Brains were rapidly removed and frozen on dry ice.
Twenty-two fvb mice were used to create the I-Long SAGE hypothalamic library (Sanger_Hypo). Six C57BL/6 were used to make the LongSAGE-Solexa hypothalamic library (Solexa_Hypo). Six other C57BL/6 mice were used to perform qPCR controls.
Collection of hypothalamic tissue
Brains were sliced in 400 μm-thick frontal sections at -12°C using a MICROM HM550 cryostat. The hypothalamus was collected bilaterally as 1 mm diameter punches centered on the perifornical nucleus from the caudal part of the paraventricular nucleus to the mammillary bodies (2 sections/animal) (Figure 2). Tissue samples were kept at -80°C until use.
Total RNA extraction
Total RNA was extracted from a pool of hypothalamic tissue using the RNeasy mini kit (QIAGEN) following manufacturer's protocol. An average of 3.4 mg of hypothalamic tissue per mice was collected giving approximately 1 μg of total RNA per mg of tissue.
The quality and quantity of total RNA were assessed with the bioanalyzer 2100 (Agilent) and with optical density (Biophotometer, Eppendorf). The ribosomic RNA 28 s/18 s ratio was 1.76 and 1.98 for the Sanger_Hypo and Solexa_Hypo libraries respectively and RIN were over 8, indicating a suitable quality for the extracted RNA. The ratio of 260/280 on the biophotometer was over 1.8.
Since we are ultimately interested in using the longSAGE-solexa technology from small samples in upcoming studies, we added an amplification step to the protocol. Briefly, 100 ng of total RNA from hypothalamic samples of C57BL/6 were amplified using the SMART™ mRNA amplification kit (Clontech) to construct the Solexa_Hypo library following manufacturer's instructions.
The efficacy of the first and second strand cDNA synthesis was evaluated and quantified by looking whether the 5'end of a long sized gene is amplified with the same amount as the 3' end using the 1.2 kb GAPDH gene (3' end primers: 5'-AAGGTCATCCCAGAGCTGAA and 5'-TGTGAGGGAGATGCTCAGTG; 5' end primers: 5'-CGTCCCGTAGACAAAATGGT and 3'-GTGGTTCACACCCATCACAA). PCR amplifications were made using platinium® Taq polymerase (Invitrogen) (PCR buffer Minus Mg 1×, dNTP 0,2 mM each, MgCl2 1,5 mM, primer 0,2 μM each, Taq 1 U, water qsp 50 μl; Primary denaturation at 94°C for 2 min, then 30 cycles of denaturation at 94°C for 30 sec, annealing at 60°C for 30 sec, extension at 72°C for 45 sec; expected sizes were 443 and 420 bp for 3' and 5' primers respectively). No difference in bands intensity was seen.
Amplification linearity was also evaluated using semi-quantitative PCR (Light Cycler®, Roche) by comparing the abundance of some hypothalamic genes before and after amplification calculated as the ratio between the level of expression of genes of interest normalized with GAPDH or cyclophillin before and after amplification. This ratio was comprised between 0.8 and 1.2 indicating that the amplification between genes was quite linear. The genes tested were prodynorphin F:TAGCTGAAGGAGAGACTGTC, R:CTGGGTTACTTGAATCCAGC; preprohypocretin F:CTAGAGCCACATCCCTGCTC, R:GGGAAGTTTGGATCAGGACA; NARP F:GCCTTTGTTGGAGAGCTCAG, R:AGAGGGCAGCTACAAGTCCA); Cyclophilin (F: CTGCACTGCCAAGACTGAATG and R: TTGCCATTCCTGGACCCAAA) and GAPDH (F: TCGTGGATCTGACGTGCCGCCTG, R: CACCACCCTGTTGCTGTAGCCGTAT).
To evaluate the level of expression for 3 well-known genes expressed in the hypothalamus and compare it to the occurence of the corresponding tags of these genes in each library, we conducted a quantitative RT-PCR using SyBr green labeling (Light Cycler®, Roche). Reverse transcription was processed from 1 μg of total RNA extracted from C57BL/6 mouse hypothalami (n = 6) using the Superscript II reverse transcriptase (Invitrogen) following manufacturer's instructions. A PCR reaction was done using specific primers for pro-Melanin Concentrating Hormone (F:GTATGCTGGGAAGAGTCTAC, R:ACGTCAAGCATATCGCTTAC), preprohypocretin (see upper) and prodynorphin (see upper). Products of PCR were gel purified and the concentration in copy number was calculated for each gene. These samples were used to built-up a standard curve. Q-PCR was then done on the hypothalamic samples and on standards. By comparing values from hypothalamic samples to the standard curves, the level of expression of these three genes was evaluated in number of copies.
LongSAGE library construction was performed from 10 μg of total RNA using the I-LongSAGE™ kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol and as described in  (Figure 1). All control steps suggested in the kit were done using GAPDH PCR primers (F:5'-TTAGCACCCCTGGCCAAGG-3'; R:5'-CTTACTCCTTGGAGGCCATG-3') and platinium® Taq polymerase (Invitrogen) (annealing of 55°C; 540 bp amplification product). The average insert size of concatemers was of 1119 bp, resulting in a mean number of 11.6 tags per concatemer. Sequencing was performed on 8448 clones by the Centre National de Séquençage (Genoscope d' Evry, France) by the SANGER method with an A3730 sequencing system. Among them, 6947 clones gave a proper sequencing results with 39324 ditags extracted. When eliminating repeated ditags, 36157 ditags were kept for subsequent analysis identifying a total of 68023 tags.
Five hundred nanograms of amplified RNA were processed to obtain the Solexa_Hypo library. Briefly, polyadenylated amplified RNA were fixed to oligo(dT) magnetic beads, first strand and second strand of cDNA were synthesized according to the first steps of the I-Long SAGE procedure used above (Invitrogen). The efficacy of the first and second strand cDNA synthesis was evaluated and quantified by PCR using GAPDH primers and platinium® Taq polymerase (Invitrogen) as described above. The next steps were performed by GATC Inc. using their own adapters to be compatible with the Solexa high throughput sequencing technology. Similarly, their method is based on NlaIII and MmeI enzymatic digestions to isolate tags (Figure 1). Sequencing was performed using Solexa Illumina sequencing technology (GATC Inc).
For the Sanger_Hypo library, we used R and the Sagenhaft library  to extract ditags and tags from Phred concatemer sequence files. For the Solexa_Hypo library, we implemented a Perl script for tag extraction from Solexa sequencing files. In both libraries, we only considered tags with all bases having a probability of erroneous call (pe) of less than 1%. This correspond to a minimal phred score Sphred = -10 log10(pe) = 20 and to a minimal Solexa sequencing score SSolexa = -10 log10(pe/(1-pe)) = 19.96. The percentage of tags with at least one base having a probability of erroneous call of less than 1% (ie Solexa score < 19.96) is of 69.59% (i.e. 11017712 tags out of 15831570 sequenced tags were of acceptable quality).
We matched tags on the mouse genome (Ensembl release 47, based on NCBI m37 assembly) by using the Megablast algorithm . Only matches with 100% identity over the whole length of the tags (21 bp) were conserved, except for estimating the "one base mismatch" rate.
We used R to obtain random samples of tags from each library and to analyze corresponding results. All the graphics were done with R.
We thank IN2P3 for their computing resources, and especially Pascal Calvat. We thank Francesca Damiola for technical advices during the construction of the Sanger_Hypo library.
Sequencing at Genoscope was supported by CNRG.
This work was supported by the Fédération de la Recherche sur le Cerveau (FRC), the EDF Foundation and the European Sleep Research Societies (ESRS-Sanofi-Synthelabo grant) to CP. This work was also partly supported by grants from the Ligue contre le cancer, and the Association pour la recherche contre le cancer (ARC) to OG. It was also supported by CNRS, UCBL. LH was supported by the French Ministry of Research and Education.
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