MiDAS exon skipping detection using RMA or PLIER summarization. ROC curves were used to identify the effect of data summarization on the detection of ASEs. ASEs were detected using MiDAS on the full core Exon 1.0 ST data set (continuous lines) using RMA (red line) or PLIER (black line). The same analysis was also applied to a subset of the core Exon 1.0 ST data set by encompassing only those gene/exon-level probe sets passing the multiple RNAs filter (dashed lines), i.e. those exons of genes associated to more than one mRNA isoform in ENSEMBL database.