Alignment of Tsp3 tetraspanins. Sequences were aligned using ClustalX 1.8. Conserved amino acids are indicated in black (>80%), dark gray (>60%) and light gray (>40%). The transmembrane domains (TM) are circled in black, the small extracellular loop (ECL1), the small intracellular loop (ICL), the large extracellular loop (ECL2) are shown in gray, purple and orange lines, respectively. ECL2 contains a CCG motif and further two conserved cysteine residues (red). These cysteine residues allow formation of two disulphide bridges crucial for the folding of ECL2. Mg: Magnaporthe grisea, Pa: Podospora anserina, Nc: Neurospora crassa, Bc: Botrytis cinerea, Ss: Sclerotinia sclerotiorum, Tr: Trichoderma reesei, Gz: Gibberella zea, An: Aspergillus niger, Ur:Uncinocarpus reesii. For a tetraspanin structural model, see Figure 1.