Alignment and phylogenetic tree of Tpl1 tetraspanins like. A. Alignment of Tpl1 tetraspanins. Sequences were aligned using ClustalX 1.8. Conserved amino acids are indicated in black (>80%), dark gray (>60%) and light gray (>40%). The transmembrane domains (TM) are circled in black, the small extracellular loop (ECL1), the small intracellular loop (ICL), the large extracellular loop (ECL2) are shown in gray, purple and orange lines, respectively. ECL2 contains two conserved cysteine residues (red). Mg: Magnaporthe grisea, Cg: Chaetomium globosum, Nc: Neurospora crassa, Pa: Podospora anserina, Sn:Stagonospora nodorum, An: Aspergillus nidulans, Ac: Aspergillus clavatus. (For a structural model, see Figure 1). B. Phylogenetic tree of fungal Tpl1 proteins. Aligned sequences were analyzed using maximum likelihood from PHYML and XP_501745 from Yarrowia lipolytica and Nce2 (YPR149W) from Saccharomyces cerevisiae as outgroups. Bootstraps values are expressed as percentage of 100 replicates.